Article
Entomology
Tran Zen B. Torres, Brian C. Prince, Alexis Robison, Claudia Ruckert
Summary: Culex mosquitoes are important vectors of mosquito-borne diseases, but effective treatments and vaccines are still lacking. Investigating the antiviral genes of Culex mosquitoes and developing gene-based vector control strategies are crucial. In this study, a Culex-optimized CRISPR/Cas9 plasmid was developed and characterized, providing a valuable tool for studying gene functions in Culex mosquito cell lines.
Review
Biotechnology & Applied Microbiology
Inaiara D. Pacheco, Linda L. Walling, Peter W. Atkinson
Summary: The order Hemiptera, which includes significant agricultural pests, has been largely overlooked in genetic control approaches due to the difficulty of introducing genetic material into these insects. However, recent advances in CRISPR/Cas9 editing have shown promise in achieving gene editing in Hemipteran species. This review discusses the challenges and opportunities for extending genetic control strategies to this agriculturally important insect order.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Si-Wei Wang, Chao Gao, Yi-Min Zheng, Li Yi, Jia-Cheng Lu, Xiao-Yong Huang, Jia-Bin Cai, Peng-Fei Zhang, Yue-Hong Cui, Ai-Wu Ke
Summary: The CRISPR/Cas9 gene editing technology has made significant progress in oncological research, providing a powerful tool for exploring the mechanism of cancer, establishing tumor models, and studying drug targets.
Review
Materials Science, Multidisciplinary
Shao Wei Hu, Tao Ding, Honghai Tang, Huiping Guo, Wenguo Cui, Yilai Shu
Summary: With the advancement of genome editing techniques, gene therapy is increasingly being used for the treatment of various human diseases. Nanomaterials have shown great potential in improving the efficiency and safety of gene editing in gene therapy. This review introduces three gene editing tools, with a particular focus on the development and optimization of the CRISPR system. It also discusses the recent application of nanobiomaterials in gene therapy, along with the prospects and remaining challenges. Lastly, future directions for gene editing tools, nanobiomaterial vectors, and gene therapy are presented.
Article
Biology
Ganna Reint, Zhuokun Li, Kornel Labun, Salla Keskitalo, Inkeri Soppa, Katariina Mamia, Eero Tolo, Monika Szymanska, Leonardo A. Meza-Zepeda, Susanne Lorenz, Artur Cieslar-Pobuda, Xian Hu, Diana L. Bordin, Judith Staerk, Eivind Valen, Bernhard Schmierer, Markku Varjosalo, Jussi Taipale, Emma Haapaniemi
Summary: Precision CRISPR gene editing can be improved by fusing DNA repair proteins with Cas9, with different fusion proteins showing varying effects based on cell type and genomic site. Optimization is necessary to account for the diverse factors contributing to locus-specific genome editing outcomes.
Review
Biotechnology & Applied Microbiology
Congting Guo, Xiaoteng Ma, Fei Gao, Yuxuan Guo
Summary: Gene editing involves precise changes to specific nucleic acid sequences. The CRISPR/Cas9 system has revolutionized gene editing, making it efficient, convenient, and programmable. However, off-target effects remain a major concern, leading to unexpected alterations in the genome. Various methods have been developed to detect and mitigate these off-target effects, advancing the precision of CRISPR/Cas9 derivatives. This review summarizes these technological advancements and discusses the challenges in managing off-target effects for future gene therapy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
J. Boutin, J. Rosier, D. Cappellen, F. Prat, J. Toutain, P. Pennamen, J. Bouron, C. Rooryck, J. P. Merlio, I. Lamrissi-Garcia, G. Cullot, S. Amintas, V. Guyonnet-Duperat, C. Ged, J. M. Blouin, E. Richard, S. Dabernat, F. Moreau-Gaudry, A. Bedel
Summary: The study reveals the genotoxicity caused by CRISPR-Cas9 leading to loss of heterozygosity changes, while also highlighting the potential safety concerns of this technology for gene therapy.
NATURE COMMUNICATIONS
(2021)
Article
Plant Sciences
Guo-Qing Song, Grace Urban, John T. Ryner, Gan-Yuan Zhong
Summary: This study evaluated chimeric editing in gene editing, using a transgenic tobacco line and a CRISPR-Cas9 editing vector. It was found that chimeric editing primarily occurred during or after cell regeneration. Further transformation was conducted to enhance editing efficiency. The findings provide valuable information for understanding gene editing in plant cells using constitutively expressed CRISPR-Cas9 and gRNAs.
Review
Cell Biology
Heba M. Mansour, Aiman S. El-Khatib
Summary: This article reviews the pathogenesis of Parkinson's disease (PD) and its association with CRISPR/Cas9 gene editing technology. It introduces the kinases encoding genes related to PD and targeted control by CRISPR/Cas9 technology. It also discusses the off-target effects of the CRISPR/Cas9 system and how to address them, as well as the challenges and future directions in kinase research for PD.
AGEING RESEARCH REVIEWS
(2023)
Article
Biochemical Research Methods
Stefan J. Tekel, Nicholas Brookhouser, Kylie Standage-Beier, Xiao Wang, David A. Brafman
Summary: The introduction of transient reporters of editing enrichment (TREE) has enabled highly efficient single-base editing of human cells using a transient episomal fluorescent reporter, allowing for rapid generation of clonal editing efficiencies exceeding 80% in biallelic or multiplexed edited isogenic human pluripotent stem cell lines within approximately 3-4 weeks.
Review
Biochemistry & Molecular Biology
Chin-Kai Chuang, Wei-Ming Lin
Summary: Cas9-sgRNA is a key tool for genome editing, with its simplicity, unique RNA-guided DNA recognition activity, and independent nuclease activities. Different variants of Cas9, in combination with sgRNA, can achieve various effects on target site breakage. Additionally, engineered enzyme activities can be introduced using Cas9 derivatives, and methods for transient expression and biosafety have been developed to address off-target effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Chengpeng Wang, Yang Li, Na Wang, Qin Yu, Yonghong Li, Junping Gao, Xiaofeng Zhou, Nan Ma
Summary: We established a platform for CRISPR/Cas9-mediated gene editing in rose using suspension cells, and successfully edited a gene involved in the ethylene signaling pathway. Our optimized CRISPR/Cas9 system provides a powerful tool for functional genomics, molecular breeding, and synthetic biology in rose.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Review
Cell Biology
Manuel M. Vicente, Miguel Chaves-Ferreira, Joao M. P. Jorge, Joao T. Proenca, Vasco M. Barreto
Summary: The repurposing of the CRISPR/Cas bacterial defense system as molecular tools has revolutionized gene editing, and efforts have been made since 2013 to decrease the frequency of unwanted mutations during CRISPR-based gene editing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Plant Sciences
Jiarui Jiang, Haitao Huang, Qian Gao, Yong Li, Haiying Xiang, Wanli Zeng, Li Xu, Xin Liu, Jing Li, Qili Mi, Lele Deng, Wenwu Yang, Jianduo Zhang, Guangyu Yang, Xuemei Li
Summary: This study investigates the broad impact of DFR knockout on various developmental phases and organs of tobacco. DFR-KO decreases catabolism and photosynthetic light reactions in tobacco leaves during the flowering stage, while increasing carbon fixation and disease resistance pathways. However, the effects of DFR-KO on tobacco growth progressively decline with time. This research provides valuable insights and theoretical foundations for tobacco breeding and the development of gene-edited strains.
Review
Pharmacology & Pharmacy
Daniel Allen, Nechama Kalter, Michael Rosenberg, Ayal Hendel
Summary: Genome engineering through targeted nucleases, especially CRISPR-Cas9, has revolutionized gene therapy research and has the potential to treat blood and immune system diseases. CRISPR-Cas9 homology-directed repair (HDR) represents a promising method for site-specific gene insertion or correction. Other methods, like viral gene addition, gene knockout through non-homologous end joining (NHEJ), and base or prime editing, also show promise but have significant limitations for treating genetic immune or blood disorders. This review aims to highlight the transformative benefits of HDR-mediated gene therapy and propose solutions for current challenges, in order to advance HDR-based gene therapy in CD34(+) hematopoietic stem progenitor cells (HSPCs).
Article
Andrology
Qing-Lai Tang, Tao Song, You-Feng Han, Bai-Bing Yang, Jian-Huai Chen, Zhi-Peng Xu, Chun-Lu Xu, Yang Xu, Wen Yu, Wei Qiu, Jiong Shi, En-Si Zhang, Yu-Tian Dai
Summary: Selective dorsal neurotomy (SDN) is a surgical treatment for primary premature ejaculation (PE) that lacks a standard surgical procedure for selecting the branches of the dorsal penile nerves. This study aimed to explore the value of intraoperative neurophysiological monitoring (IONM) of penile sensory-evoked potential (PSEP) for standardizing SDN procedures. The results showed that IONM may improve short-term therapeutic effectiveness and reduce complications in SDN surgeries.
ASIAN JOURNAL OF ANDROLOGY
(2023)
Article
Pathology
Xin Zhang, Xiaoxi Fan, Changbo Sun, Liang Wang, Yuan Miao, Liming Wang, Peng Yang, Yang Xu, Xue Ren, Xue Wu, Shun Xu
Summary: In this study, NGS analysis was used to diagnose MLA patients with MPLAs and IPMs. By incorporating a predefined NGS criterion, accurate classification of IPMs was achieved. A refined algorithm was developed that combined NGS and histologic results to correctly diagnose MPLAs and IPMs. This NGS-based algorithm showed promising potential in complementing traditional CHA-based MLA diagnosis and helping determine patient staging and treatment.
JOURNAL OF PATHOLOGY CLINICAL RESEARCH
(2023)
Article
Medicine, General & Internal
Weixin Zhao, Ailing Song, Yang Xu, Qian Wu, Cuicui Liu, Jiani C. C. Yin, Qiuxiang Ou, Xue Wu, Yang Shao, Xinmin Zhao
Summary: This retrospective study analyzed next-generation sequencing (NGS) data from 1025 non-small cell lung cancer (NSCLC) patients with compound epidermal growth factor receptor (EGFR) mutations. Different subtypes of compound EGFR mutations were associated with distinct clinical features, genetic architectures, and prognostic outcomes. Rare mutation-dominant subtypes with variations of uncertain significance (VUSs) residing in the kinase domain had poor clinical outcomes and implied disruptions in kinase domain regulation/function.
Article
Materials Science, Multidisciplinary
Feifei Huang, Yi Qin, Hongbo Zhang, Qingrui Wang, Dan Li, Zhaogui Lai, Lei Wen, Hai Chang, Ying Jin
Summary: The potential-dependent mechanism of oxide film formation on Ti-6Al-3Nb-2Zr-1Mo in 0.5 M H2SO4 was investigated within a potential range of -0.80-5.00 V. The thicknesses of oxide films and the valence states of Ti, Al, Nb, and Mo were found to increase with increasing potential to 3.00 V, while surface roughness showed an opposite trend. The results indicated that higher potentials in the passive region led to better protection and uniformity of the oxide film. A schematic considering the effect of alloying elements within oxide films formed at different applied potentials on Ti-6Al-3Nb-2Zr-1Mo was established.
Article
Chemistry, Physical
Hongbo Zhang, Christofer Leygraf, Lei Wen, Feifei Huang, Hai Chang, Ying Jin
Summary: The fracture mechanism of hydrogen charged Ti-6Al-4V was investigated through a multianalytical approach. The study found that the distribution and cracks of hydrides influence the fracture mode of the Ti-6A-4V alloy.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Y. Chen, H. Wang, Q. Ni, T. Wang, C. Bao, Y. Geng, Y. Lu, Y. Cao, Y. Li, L. Li, Y. Xu, W. Sun
Summary: B cells play a crucial role in periodontitis by regulating immune response, inducing tissue destruction, and eliminating periodontal pathogens. However, their role in mesenchymal stem cell (MSC) differentiation to osteoblasts in periodontitis has been poorly studied.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Medicine, Research & Experimental
Xin Chen, Kaixuan Bai, Yu Zhang, Yang Xu, Yinghao Huo, Sha Wang, Yueli Zou, Xuejiao Qi, Rongyun Guo, Qiuxiang Ou, Dengxiang Liu, Shaohua Yin, Shubo Chen, Hui Bu
Summary: In this study, it was found that cerebrospinal fluid circulating tumor DNA could sensitively detect molecular markers and metastasis-related mechanisms in gastric cancer patients with leptomeningeal metastases, providing new clues for prognosis estimation and clinical assessment.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Oncology
Yongkan Bai, Xiang Liu, Limin Zheng, Song Wang, Junli Zhang, Shi Xiong, Pengfei Zhang, Zichen Jiao, Gefei Zhao, Chu Zhou, Jiaohui Pang, Yang Xu, Qiuxiang Ou, Yuan Mao, Louqian Zhang
Summary: In this study, the genomic profiles of NSCLC patients with cEGFR and uEGFR mutations were analyzed using targeted next-generation sequencing. The results showed that uEGFR-positive patients were more likely to have co-occurring genetic alterations in the Hippo pathway and a higher TMB. Smoking-related mutations were also enriched in uEGFR-positive patients. Subgroup analyses revealed potential prognostic biomarkers and significant differences in mutational profiles among patients with different EGFR sub-type mutations.
Letter
Oncology
Xueting Qin, Yujun Bai, Shizhen Zhou, Hongjin Shi, Xiaoli Liu, Song Wang, Xiaoying Wu, Jiaohui Pang, Xi Song, Xiaojun Fan, Qiuxiang Ou, Yang Xu, Hua Bao, Li Li, Jun Li, Yang Shao, Shuanghu Yuan
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Letter
Oncology
Kewen He, Li Li, Aijie Li, Yang Xu, Jiaohui Pang, Dianbin Mu, Jie Ma, Hong Ge, Aurian Maleki, Xueting Qin, Xian Zhang, Qiuxiang Ou, Yang Shao, Jinming Yu, Shuanghu Yuan
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Editorial Material
Biochemistry & Molecular Biology
Weidong Wang, Wang Lv, Hui Wang, Yang Xu, Junrong Yan, Han-Ming Shen, Liqun Shan, Jian Hu
Article
Oncology
Jin Li, Xiaohong Xue, Yan Zhang, Fengna Ding, Wenyan Wu, Cuicui Liu, Yang Xu, Hanlin Chen, Qiuxiang Ou, Yang Shao, Xinjun Li, Fei Wu, Xiaohua Wu
Summary: This multi-center study in Chinese cervical cancer patients highlights the immunologic and genomic differences between squamous cell carcinoma (SCC) and adenocarcinoma (AC). The study suggests that SCC may have better immunotherapy efficacy, while AC may be more favorable for targeted therapy. These findings are significant for guiding treatment strategies for different types of cervical cancer.
GYNECOLOGIC ONCOLOGY
(2023)
Article
Dermatology
Meng Tao, Min Li, Yue Zhang, Duoduo Gu, Ruoxin Pan, Yang Xu
Summary: This study found that individuals with rosacea have different facial erythema distribution patterns, which may be related to skin types. The erythema distribution pattern of rosacea was divided into peace signs, wing shapes, and neither of the two patterns. The peace sign pattern was more common in individuals with darker skin tones, while the wing shape pattern was associated with the lateral parts of the cheeks. This has important implications for understanding the pathogenesis and treatment of rosacea.
ARCHIVES OF DERMATOLOGICAL RESEARCH
(2023)
Article
Respiratory System
Yu Yao, Huaping Yang, Bo Zhu, Song Wang, Jiaohui Pang, Xiaoying Wu, Yang Xu, Junli Zhang, Jinfeng Zhang, Qiuxiang Ou, Hui Tian, Zheng Zhao
Summary: This study found that MET tyrosine kinase domain (TKD) mutations were detected in both baseline and patients treated with TKIs in non-small-cell lung cancer (NSCLC) patients. MET-H1094Y may be associated with acquired resistance after recurrent EGFR-TKIs treatment. Preliminary data suggests that EGFR-mutated NSCLC patients who acquired MET-V1092I or MET-H1094Y may benefit from combinatorial therapy with EGFR-TKI and MET-TKI, providing new insights into personalized medical treatment.
RESPIRATORY RESEARCH
(2023)
Article
Medicine, Research & Experimental
Wei Wei, Fangfang Shi, Yang Xu, Yang Jiao, Ying Zhang, Qiuxiang Ou, Xue Wu, Lingyi Yang, Jinhuo Lai
Summary: Next-generation sequencing was used to investigate molecular features in NSCLC patients with pathogenic/likely pathogenic ATM/ATR mutations. The results were validated using an external cohort of NSCLC patients.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)