期刊
ISME JOURNAL
卷 15, 期 7, 页码 1971-1986出版社
SPRINGERNATURE
DOI: 10.1038/s41396-021-00898-x
关键词
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资金
- University of Wisconsin-Office of the Vice Chancellor for Research and Graduate Education
- University of Wisconsin-Department of Bacteriology
- University of Wisconsin-College of Agriculture and Life Sciences
- United States National Science Foundation [DEB-1030242, DEB-0842253]
- U.S. Department of Energy Joint Genome Institute (JGI) through a JGI-Community Science Program [CSP 2796]
- Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231]
- United States National Science Foundation via an INSPIRE award [DEB-1344254]
- Wisconsin Alumni Research Foundation at UW-Madison
- Leibniz Institute for Freshwater Ecology and Inland Fisheries' International Postdoctoral Research Fellowship
- German Research Foundation through the LimnoScenES project [AD 91/22-1]
- NSF-REU award for Summer 2020
- Natural Sciences and Engineering Research Council of Canada (NSERC)
Through genome-resolved metagenomics and environmental analyses, this study revealed a contrast in community composition and metabolic potential between the oxygenated upper layers with typical freshwater taxa and deep anoxic waters with Archaea and uncultured Candidate Phyla. The genomic capacity for nitrogen and sulfur cycling was found to be abundant in microbial genomes recovered from anoxic waters, indicating their contribution to recycling nutrients and greenhouse gases in surface layers. This study provides insight into the role of aquatic microbial genomics in tropical freshwater lakes, particularly in the face of climate change bringing increased stratification and anoxia.
Lake Tanganyika (LT) is the largest tropical freshwater lake, and the largest body of anoxic freshwater on Earth's surface. LT's mixed oxygenated surface waters float atop a permanently anoxic layer and host rich animal biodiversity. However, little is known about microorganisms inhabiting LT's 1470 meter deep water column and their contributions to nutrient cycling, which affect ecosystem-level function and productivity. Here, we applied genome-resolved metagenomics and environmental analyses to link specific taxa to key biogeochemical processes across a vertical depth gradient in LT. We reconstructed 523 unique metagenome-assembled genomes (MAGs) from 34 bacterial and archaeal phyla, including many rarely observed in freshwater lakes. We identified sharp contrasts in community composition and metabolic potential with an abundance of typical freshwater taxa in oxygenated mixed upper layers, and Archaea and uncultured Candidate Phyla in deep anoxic waters. Genomic capacity for nitrogen and sulfur cycling was abundant in MAGs recovered from anoxic waters, highlighting microbial contributions to the productive surface layers via recycling of upwelled nutrients, and greenhouse gases such as nitrous oxide. Overall, our study provides a blueprint for incorporation of aquatic microbial genomics in the representation of tropical freshwater lakes, especially in the context of ongoing climate change, which is predicted to bring increased stratification and anoxia to freshwater lakes.
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