Article
Infectious Diseases
Young-Min Kim, Hyosuk Son, Seong-Cheol Park, Jong-Kook Lee, Mi-Kyeong Jang, Jung Ro Lee
Summary: Biofilms are resistant to antibiotics and are a major source of persistent and recurring infections by clinically important pathogens. Researchers are developing new and effective antibiofilm agents, among which antimicrobial peptides have attracted interest. This study evaluated the effects of 10 synthetic peptides on growth inhibition, inhibition of biofilm formation, and biofilm elimination in drug-resistant Pseudomonas aeruginosa. The results showed that WIK-14 and WIKE-14 peptides could provide novel therapeutic drugs to overcome multidrug resistance in biofilm-associated infections.
Review
Microbiology
Xuejie Li, Nixuan Gu, Teng Yi Huang, Feifeng Zhong, Gongyong Peng
Summary: Pseudomonas aeruginosa (P. aeruginosa) is a gram-negative pathogenic microorganism known for its virulence factors, biofilm forming capability, and antimicrobial resistance. Despite being underestimated, it is a foodborne pathogen that can be found in various food groups. Chemical preservatives used to suppress microbial growth in food can pose food safety concerns. Therefore, finding effective and natural alternative antimicrobial agents for food processing is crucial. This review focuses on recent advances in food safety related to P. aeruginosa, including antimicrobial resistance, virulence factors, and prevention measures.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Yun-Qi Song, Su Min Kyung, Suji Kim, Gun Kim, So Yeong Lee, Han Sang Yoo
Summary: Pseudomonas aeruginosa is a major pathogen in nosocomial infections, which are difficult to handle due to its biofilm-forming ability and carbapenem resistance. Antimicrobial peptides, such as RP557, have shown effectiveness in inhibiting biofilms. Our study assessed the effectiveness of RP557 on carbapenem-resistant P. aeruginosa planktonic cells and biofilms, providing a potential treatment option for carbapenem-resistant P. aeruginosa infection. This treatment could be helpful in managing severe nosocomial infections caused by carbapenem-resistant bacteria and improving patients' survival rates.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Mario Gajdacs, Zoltan Barath, Krisztina Karpati, Dora Szabo, Donatella Usai, Stefania Zanetti, Matthew Gavino Donadu
Summary: The study found a relationship between biofilm formation and expression of virulence factors with multidrug resistance in Pseudomonas aeruginosa. However, there were no significant differences in virulence factor expression between multidrug-resistant and non-multidrug-resistant isolates, and no correlations were seen between the rate of biofilm formation, pigment production, or motility. Understanding the interplay between drug resistance mechanisms, biofilm formation, and virulence is crucial for managing chronic bacterial infections.
Article
Immunology
Laia Fernandez-Barat, Nil Vazquez Burgos, Victoria Alcaraz, Leticia Bueno-Freire, Ruben Lopez-Aladid, Roberto Cabrera, Albert Gabarrus, Andrea Palomeque, Patricia Oscanoa, Adrian Ceccato, Ana Motos, Rosanel Amaro, Thierry Bernardi, Christian Provot, Alba Soler-Comas, Laura Munoz, Jordi Vila, Antoni Torres
Summary: The aim of this study was to validate and identify additional applications of the BioFilm Ring Test (BRT) for Pseudomonas aeruginosa (PA) isolates from patients with bronchiectasis (BE), and evaluate its relationship with antimicrobial stewardship. The results showed that a BPI value below 14.75 at 5 hours predicted the mucoid PA phenotype. In conclusion, the BRT has the potential to reveal biofilm features with clinical implications.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Medicinal
Ali Hazim Abdulkareem, Anmar Kamil Alalwani, Mohammed Mukhles Ahmed, Safaa Abed Latef Al-Meani, Mohammed Salih Al-Janaby, Al-Moghira Khairi Al-Qaysi, Ali Ibrahim Edan, Hasan Falah Lahij
Summary: The increasing disparity between antimicrobial resistance and the development of new antimicrobials continues to pose a significant global health concern. This study found that goldenrod extract has the ability to inhibit the production of extended-spectrum beta-lactamases (ESBLs) and biofilm formation in ESBL-Pseudomonas aeruginosa.
Review
Biochemistry & Molecular Biology
Jonathan Chung, Shafinaz Eisha, Subin Park, Amanda J. Morris, Isaac Martin
Summary: In cystic fibrosis (CF), pulmonary infection with Pseudomonas aeruginosa is a major cause of morbidity and mortality. The biofilm mode of growth of P. aeruginosa, with its self-secreted exopolysaccharides (EPSs), provides physical protection against antibiotics and leads to phenotypic heterogeneity. Current antimicrobials are inadequate in eradicating P. aeruginosa infection, thus there is a need for new therapies. This review focuses on the investigation and exploitation of the three biofilm-associated EPSs as potential therapeutic targets for combating pulmonary infection with P. aeruginosa in CF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Derek Fleming, Brandon Niese, Whitni Redman, Emily Vanderpool, Vernita Gordon, Kendra P. Rumbaugh
Summary: The absence of the exopolysaccharides Pel and Psl in Pseudomonas aeruginosa did not significantly affect the severity or wound closure rates of wound infections in a mouse model. However, the size and distribution of biofilm aggregates in the wound tissue were different, and the mutants' ability to survive antibiotic treatment was impaired.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Magdalena Ratajczak, Dorota Kaminska, Jolanta Dlugaszewska, Marzena Gajecka
Summary: The study assessed the antibiotic resistance and biofilm formation of bacteria in pharmaceutical production sites, finding that the majority of P. aeruginosa strains exhibited resistance to antibiotics and strong biofilm-forming ability, as well as containing genes encoding various virulence factors.
Article
Chemistry, Multidisciplinary
Xiaodie Yuan, Xia Zhao, Yuan Lin, Zhaohui Su
Summary: This study synthesized PDA nanoparticles of various sizes and introduced different surface groups to investigate their penetration and accumulation abilities in biofilms. The PDA nanoparticles of approximately 60 nm size showed higher penetration and accumulation abilities, and the positively charged amine groups were more effective in enhancing the interactions with the biofilm compared to carboxyl or methoxy groups. The PDA60 nanoparticles with amine surface groups also exhibited good photothermal properties.
Article
Chemistry, Multidisciplinary
Baixing Chen, Luis Ponce Benavente, Marco Chitto, Jacek K. Wychowaniec, Virginia Post, Matteo D'Este, Caroline Constant, Stephan Zeiter, Wenli Feng, Mercedes Gonzalez Moreno, Andrej Trampuz, Jeroen Wagemans, Jolien Onsea, R. Geoff Richards, Rob Lavigne, T. Fintan Moriarty, Willem-Jan Metsemakers
Summary: The study aimed to develop a dual antibiotic-phage delivery system using a hydrogel and alginate microbeads to treat multi-drug resistant Pseudomonas aeruginosa infection. The results showed that the combination of phages and meropenem had enhanced antibacterial activity against bacterial biofilms. The dual delivery system reduced the development of phage resistance and exposure to the immune system.
JOURNAL OF CONTROLLED RELEASE
(2023)
Review
Microbiology
Daniel Ruben Akiola Sanya, Djamila Onesime, Grazia Vizzarro, Nicolas Jacquier
Summary: The opportunistic human pathogen Pseudomonas aeruginosa is responsible for various infections, especially in hospitalized patients where drug-resistant strains can contribute to in-hospital mortality. Chronic respiratory system infections in cystic fibrosis patients caused by this pathogen are particularly concerning due to difficulty in treatment. Pseudomonas aeruginosa utilizes cell-associated and secreted virulence factors in its pathogenesis, and recent advances highlight the understanding of its pathogenicity and efforts towards new drug targets and therapeutic strategies.
Article
Pharmacology & Pharmacy
Davoodbasha MubarakAli, Kannappan Arunachalam, Murugan Lakshmanan, Bazigha Badar, Jung-Wan Kim, Sang-Yul Lee
Summary: Biofilm-related nosocomial infections can cause various life-threatening infections. In this study, hydroxyapatite nanoparticles (HANPs) were synthesized using a co-precipitation method followed by microwave treatment, and their ability to inhibit the biofilm formation of Pseudomonas aeruginosa ATCC 10145 was investigated. The results showed that the synthesized HANPs effectively inhibited biofilm formation, suggesting their potential use in controlling biofilm-related infections caused by Pseudomonas aeruginosa.
Article
Infectious Diseases
Feifei Yang, Chunhong Liu, Jian Ji, Wenjun Cao, Baixing Ding, Xiaogang Xu
Summary: The Pseudomonas aeruginosa strains isolated from patients with aural infections showed low rates of resistance to most antibiotics but high resistance rates to quinolones. These strains exhibited strong biofilm formation ability and low susceptibility to biofilm eradication, indicating the need for more effective drugs and treatment methods to combat these infections.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Microbiology
Sara Masihzadeh, Mansour Amin, Zahra Farshadzadeh
Summary: This study investigated the antibiofilm activity of the WLBU2 peptide against Pseudomonas aeruginosa isolates. Results showed that WLBU2 exhibited strong inhibitory and eradication potency against MDR and carbapenem-resistant strains of P. aeruginosa, both in vitro and in vivo.