期刊
ENVIRONMENTAL TOXICOLOGY
卷 36, 期 6, 页码 1031-1042出版社
WILEY
DOI: 10.1002/tox.23102
关键词
endocrine disruptor; epigenetic; glyphosate-based herbicide; intergenerational effect; non-monotonic dose response; thyroid hormone
资金
- Fundacao Araucaria - FA (Parana, Brazil) [FA09/2016]
- CAPES - AUXPE Pro-integracao [3160/2013-98]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [2013/26851-7, 2018/22763-0, 2017/07053-3]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior [18952-12-7]
- Pro-Integracao [55/2013]
This study found that animals exposed to glyphosate-based herbicides may experience intergenerational alterations in key regulators of cellular thyroid hormone balance and epigenetic controllers, indicating a possible endocrine disruption effect of the herbicide based on epigenetic changes.
Agrochemicals became a public health concern due to increased human exposure and possible endocrine disruption effects in several organs, including the brain. Thyroid hormones controls neurodevelopment, which turn them sensitive to endocrine disruptors (EDs). In this work, we evaluated the effect of glyphosate-based herbicides (GBH) as an intergenerational endocrine disrupter on thyroid homeostasis in cerebellar cells. Female pregnant Wistar rats were exposed to Roundup Transorb (R) solution at 5 and 50 mg/kg/day, from gestation day 18 to post-natal day 5 (P5). Cerebellum of male offspring was used to evaluate gene expression. The mRNA levels of thyroid hormone receptors, hormonal conversion enzymes, hormone transporters, as well as, de novo epigenetic regulators were altered, with some of these genes presenting a non-monotonic dose response. Furthermore, metabolomic profile correlation with tested dose demonstrated altered metabolic profile, in agreement with cerebellar gene alterations. Moreover, cerebellar primary cultures exposed to non-toxic GBH concentration presented a decrease level in glial fibrillary acidic protein, a protein regulated by endocrine signals. In conclusion, our results indicate that animals exposed to non-toxic GBH doses during perinatal phase carry intergenerational alterations in key regulators of cellular thyroid hormone homeostasis and epigenetic controllers in adulthood, indicating the possible ED effect of GBH based on epigenetic alterations.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据