4.7 Article

Cellulose nanofibers production using a set of recombinant enzymes

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CARBOHYDRATE POLYMERS
卷 256, 期 -, 页码 -

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ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2020.117510

关键词

Cellulose nanofiber; Sugarcane bagasse; Enzyme; Sonication; CNF

资金

  1. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2015/13684-0, 2018/237691]
  2. Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [423693/2016-6, 303988/2016-9]

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In this study, a combination of three different recombinant enzymes was used to enhance cellulose fibrillation and produce CNF with improved properties. The cooperative action of hydrolytic and oxidative enzymes prior to sonication resulted in longer and more thermostable CNFs.
Cellulose nanofibers (CNF) are renewable and biodegradable nanomaterials with attractive barrier, mechanical and surface properties. In this work, three different recombinant enzymes: an endoglucanase, a xylanase and a lytic polysaccharide monooxygenase, were combined to enhance cellulose fibrillation and to produce CNF from sugarcane bagasse (SCB). Prior to the enzymatic catalysis, SCB was chemically pretreated by sodium chlorite and KOH, while defibrillation was accomplished via sonication. We obtained much longer (mu m scale length) and more thermostable (resisting up to 260 degrees C) CNFs as compared to the CNFs prepared by TEMPO-mediated oxidation. Our results showed that a cooperative action of the set of hydrolytic and oxidative enzymes can be used as a green treatment prior to the sonication step to produce nanofibrillated cellulose with advanced properties.

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