期刊
EMBO JOURNAL
卷 36, 期 1, 页码 25-41出版社
WILEY-BLACKWELL
DOI: 10.15252/embj.201694264
关键词
epiboly; hydrodynamics; mechanics; morphogenesis; zebrafish
资金
- Confocal Microscopy Unit from IBMB-PCB
- Advanced Digital Microscopy Core Facility from IRB Barcelona
- Consolidated Groups Program of the Generalitat de Catalunya
- DGI Grant from Ministry of Economy and Competitivity of Spain
- Consolider Grant from Ministry of Economy and Competitivity of Spain
The principles underlying the biomechanics of morphogenesis are largely unknown. Epiboly is an essential embryonic event in which three tissues coordinate to direct the expansion of the blastoderm. How and where forces are generated during epiboly, and how these are globally coupled remains elusive. Here we developed a method, hydrodynamic regression (HR), to infer 3D pressure fields, mechanical power, and cortical surface tension profiles. HR is based on velocity measurements retrieved from 2D+T microscopy and their hydrodynamic modeling. We applied HR to identify biomechanically active structures and changes in cortex local tension during epiboly in zebrafish. Based on our results, we propose a novel physical description for epiboly, where tissue movements are directed by a polarized gradient of cortical tension. We found that this gradient relies on local contractile forces at the cortex, differences in elastic properties between cortex components and the passive transmission of forces within the yolk cell. All in all, our work identifies a novel way to physically regulate concerted cellular movements that might be instrumental for the mechanical control of many morphogenetic processes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据