期刊
BMC GENOMICS
卷 22, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12864-021-07378-8
关键词
Gossypium hirsutum; WAK genes; Expression analysis
资金
- State Key Laboratory of Cotton Biology Open Fund [CB2019A03, 2019A09, CB2020A12]
- National Natural Science Foundation of China [31872175]
- Fundamental Research Funds for the Central Universities [GK202001004]
- Natural Science Basic Research Plan in Shaanxi Province of China [2018JZ3006, 2019JQ-062]
- Shaanxi Youth Entrusted Talents Program [20190205]
- Shaanxi Postdoctoral Project [2018BSHYDZZ76]
- Construction project of selenium-rich crop planting demonstration base in Shaanxi province [[2017]-01-09]
- Comprehensive Scientific research Fund project of Xianyang Normal University [XSYK20002]
- Young Elite Scientists Sponsorship Program by CAST [2019-2021QNRC001]
The study identified 29 GhWAK genes in Gossypium hirsutum, showing that these genes mainly originated from whole genome duplication (WGD) and were mainly under purifying selection. 97% of GhWAK genes highly expressed in cotton fibers and ovules, with potential hormone response elements.
BackgroundWall-associated kinases (WAK), one of the receptor-like kinases (RLK), function directly in the connection and communication between the plant cell wall and the cytoplasm. WAK genes are highly conserved and have been identified in plants, such as rice, but there is little research on the WAK gene family in cotton.ResultsIn the present study, we identified 29 GhWAK genes in Gossypium hirsutum. Phylogenetic analysis showed that cotton WAK proteins can be divided into five clades. The results of synteny and Ka/Ks analysis showed that the GhWAK genes mainly originated from whole genome duplication (WGD) and were then mainly under purifying selection. Transcriptome data and real-time PCR showed that 97% of GhWAK genes highly expressed in cotton fibers and ovules. beta -glucuronidase (GUS) staining assays showed that GhWAK5 and GhWAK16 expressed in Arabidopsis leaf trichomes. Fourteen GhWAK genes were found to possess putative gibberellin (GA) response elements in the promoter regions, 13 of which were significantly induced by GA treatment. Ten GhWAK genes contained auxin (IAA) response elements and the expression level of nine GhWAKs significantly increased under auxin treatment.ConclusionsWe provide a preliminary analysis of the WAK gene family in G. hirsutum, which sheds light on the potantial roles of GhWAK genes in cotton fiber cell development. Our data also provides a useful resource for future studies on the functional roles of GhWAK genes.
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