CRISPR-based DNA and RNA detection with liquid-liquid phase separation

Nonspherical Coacervate Shapes in an Enzyme-Driven Active System

(2020) Willem Kasper Spoelstra et al.   LANGMUIR

Biomolecular Phase Separation: From Molecular Driving Forces to Macroscopic Properties

(2020) Gregory L. Dignon et al.   Annual Review of Physical Chemistry

CRISPR–Cas12-based detection of SARS-CoV-2

(2020) James P. Broughton et al.   NATURE BIOTECHNOLOGY

Enzymatic degradation of liquid droplets of DNA is modulated near the phase boundary

(2020) Omar A. Saleh et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities

(2020) Jeanne E. van Dongen et al.   BIOSENSORS & BIOELECTRONICS

CRISPR-Cas12a-Based Nucleic Acid Amplification-Free DNA Biosensor via Au Nanoparticle-Assisted Metal-Enhanced Fluorescence and Colorimetric Analysis

(2020) Jin-Ha Choi et al.   NANO LETTERS

Mechanistic Insights into the cis- and trans-Acting DNase Activities of Cas12a

(2019) Daan C. Swarts et al.   MOLECULAR CELL

Naked-Eye Detection of Grapevine Red-Blotch Viral Infection Using a Plasmonic CRISPR Cas12a Assay

(2019) Yongya Li et al.   ANALYTICAL CHEMISTRY

SHERLOCK: nucleic acid detection with CRISPR nucleases

(2019) Max J. Kellner et al.   Nature Protocols

HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

(2019) Linxian Li et al.   ACS Synthetic Biology

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA

(2018) Shi-Yuan Li et al.   CELL RESEARCH

Phase Behavior and Salt Partitioning in Polyelectrolyte Complex Coacervates

(2018) Lu Li et al.   MACROMOLECULES

Physical principles of intracellular organization via active and passive phase transitions

(2018) Joel Berry et al.   REPORTS ON PROGRESS IN PHYSICS

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

DNA-induced liquid phase condensation of cGAS activates innate immune signaling

(2018) Mingjian Du et al.   SCIENCE

Direct observation of DNA target searching and cleavage by CRISPR-Cas12a

(2018) Yongmoon Jeon et al.   Nature Communications

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

(2018) Lucas B. Harrington et al.   SCIENCE

Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a

(2017) Daan C. Swarts et al.   MOLECULAR CELL

RNA targeting with CRISPR–Cas13

(2017) Omar O. Abudayyeh et al.   NATURE

RNA phase transitions in repeat expansion disorders

(2017) Ankur Jain et al.   NATURE

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Biology and Applications of CRISPR Systems: Harnessing Nature’s Toolbox for Genome Engineering

(2016) Addison V. Wright et al.   CELL

RNA-Based Coacervates as a Model for Membraneless Organelles: Formation, Properties, and Interfacial Liposome Assembly

(2016) William M. Aumiller et al.   LANGMUIR

Sequence Determinants of Intracellular Phase Separation by Complex Coacervation of a Disordered Protein

(2016) Chi W. Pak et al.   MOLECULAR CELL

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

(2016) Alexandra East-Seletsky et al.   NATURE

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

(2016) Omar O. Abudayyeh et al.   SCIENCE

Quantifying Protein Diffusion and Capture on Filaments

(2015) Emanuel Reithmann et al.   BIOPHYSICAL JOURNAL

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

(2015) Sergey Shmakov et al.   MOLECULAR CELL

Polymer physics of intracellular phase transitions

(2015) Clifford P. Brangwynne et al.   Nature Physics

Liquid-Liquid Phase Separation in Biology

(2014) Anthony A. Hyman et al.   Annual Review of Cell and Developmental Biology

Interfacial Tension of Polyelectrolyte Complex Coacervate Phases

(2014) Jian Qin et al.   ACS Macro Letters

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

Binodal Compositions of Polyelectrolyte Complexes

(2010) Evan Spruijt et al.   MACROMOLECULES