Review
Cell Biology
Mary Akinyi Nyonda, Jean-Baptiste Boyer, Lucid Belmudes, Aarti Krishnan, Paco Pino, Yohann Coute, Mathieu Brochet, Thierry Meinnel, Dominique Soldati-Favre, Carmela Giglione
Summary: Acetyl-CoA plays crucial roles in post-translational modification of proteins and cellular metabolism, with AT1 and NAT8 homologs identified in Toxoplasma gondii and Plasmodium berghei parasites, and widespread N-terminal acetylation observed in both species.
JOURNAL OF CELL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Dounia Ben Amar, Karine Thoinet, Benjamin Villalard, Olivier Imbaud, Clelia Costechareyre, Loraine Jarrosson, Florie Reynaud, Julia Novion Ducassou, Yohann Coute, Jean-Francois Brunet, Valerie Combaret, Nadege Corradini, Celine Delloye-Bourgeois, Valerie Castellani
Summary: Using experimental models mimicking the embryonic context and proteomic/transcriptomic analyses, this study demonstrates that signals released by embryonic sympathetic ganglia can induce the transformation of neuroblastoma cells and activate gene programs promoting metastasis.
NATURE COMMUNICATIONS
(2022)
Article
Biology
Helena Dragic, Audrey Barthelaix, Cedric Duret, Simon Le Goupil, Hadrien Laprade, Sophie Martin, Sabine Brugiere, Yohann Coute, Christelle Machon, Jerome Guitton, Justine Rudewicz, Paul Hofman, Serge Lebecque, Cedric Chaveroux, Carole Ferraro-Peyret, Toufic Renno, Serge N. Manie
Summary: The glucose-requiring hexosamine biosynthetic pathway (HBP) and the coat complex II (COPII) play a crucial role in the survival of lung adenocarcinoma (LUAD) cells in low glucose conditions. Up-regulation of HBP resists the production of abnormal proteins induced by low glucose, while up-regulation of COPII restores the cell surface expression of certain glycoproteins.
LIFE SCIENCE ALLIANCE
(2022)
Article
Cell Biology
Christopher Swale, Valeria Bellini, Matthew W. Bowler, Nardella Flore, Marie-Pierre Brenier-Pinchart, Dominique Cannella, Lucid Belmudes, Caroline Mas, Yohann Coute, Fabrice Laurent, Artur Scherf, Alexandre Bougdour, Mohamed-Ali Hakimi
Summary: The Apicomplexa, a phylum of single-celled protozoa, cause severe parasitic diseases in humans and animals. Current therapeutics are limited by their efficacy and resistance. In this study, altiratinib, originally developed for glioblastoma, is identified as a potential drug candidate with broad spectrum activity against apicomplexans. Altiratinib specifically targets the TgPRP4K of T. gondii and inhibits the development of intracellular parasites. The molecular basis of the inhibitory mechanism and species selectivity is further elucidated.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Oncology
Valentina Lukinovic, Simone Hausmann, Gael S. Roth, Clement Oyeniran, Tanveer Ahmad, Ning Tsao, Joshua R. Brickner, Alexandre G. Casanova, Florent Chuffart, Ana Morales Benitez, Jessica Vayr, Rebecca Rodell, Marianne Tardif, Pascal W. T. C. Jansen, Yohann Coute, Michiel Vermeulen, Pierre Hainaut, Pawel K. Mazur, Nima Mosammaparast, Nicolas Reynoird
Summary: This study identifies SMYD3 as a major regulator of small cell lung cancer (SCLC) sensitivity to alkylating chemotherapy and demonstrates that SMYD3 inhibition restores SCLC vulnerability to alkylating agents. These findings provide a rationale for targeting SMYD3 to improve responses to chemotherapy in SCLC.
Article
Multidisciplinary Sciences
Sebastien Durand, Marion Bruelle, Fleur Bourdelais, Bigitha Bennychen, Juliana Blin-Gonthier, Caroline Isaac, Aurelia Huyghe, Sylvie Martel, Antoine Seyve, Christophe Vanbelle, Annie Adrait, Yohann Coute, David Meyronet, Frederic Catez, Jean-Jacques Diaz, Fabrice Lavial, Emiliano P. Ricci, Francois Ducray, Mathieu Gabut
Summary: The study reveals that RSL24D1 is highly expressed in both mouse and human pluripotent stem cells and is involved in the biogenesis of 60S subunits. Depletion of RSL24D1 impairs global translation, particularly of pluripotency factors and components from the Polycomb Repressive Complex 2 (PRC2), leading to altered self-renewal and lineage commitment choices in embryonic stem cells.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
David da Silva Barreira, Julie Laurent, Jessica Lourenco, Julia Novion Ducassou, Yohann Coute, Jean Guzzo, Aurelie Rieu
Summary: Biofilms are a major concern in the food industry and healthcare. Probiotic membrane vesicles (MVs) have shown strong antibiofilm activity against Salmonella enterica serovar Enteritidis (S. Enteritidis) without affecting bacterial growth. The antibiofilm properties of the MVs were found to be associated with specific proteins, particularly peptidoglycan hydrolases (PGHs).
SCIENTIFIC REPORTS
(2023)
Article
Food Science & Technology
Marie Courcon, Cedric Badiou, Mathilde Louwagie, Sibyle Etievant, Michel Jaquinod, Gerard Lina, Virginie Brun
Summary: This study presents a mass-spectrometry-based proteomics workflow for the analysis of Staphylococcus aureus superantigenic toxins in menstrual fluid. The toxins were detected in varying concentrations in samples from patients with mTSS and healthy women.
Editorial Material
Clinical Neurology
Juliette Svahn, Laurent Coudert, Nathalie Streichenberger, Alexandra Kraut, Alice Gravier-Dumonceau-Mazelier, Ludivine Rotard, Laurence Calemard-Michel, Rita Menassa, Elisabeth Errazuriz-Cerda, Lara Chalabreysse, Alexis Osseni, Christophe Vial, Laurentiu Jomir, Francois Tronc, Do Le Duy, Emilien Bernard, Vincent Gache, Yohann Coute, Vincent Jacquemond, Laurent Schaeffer, Pascal Leblanc
Summary: This study reports a case of acquired autoimmune rippling muscle disease (iRMD) associated with thymoma and AchR antibody negativity. The patient was found to have circulating MURC/Cavin-4 autoantibodies, and the histological features of the patient's muscles were similar to those commonly reported in caveolinopathies. After tumor resection and immunotherapy, the levels of autoantibodies significantly decreased, the rippling phenotype disappeared completely, and the patient achieved full remission. Therefore, MURC/Cavin-4 autoantibodies may play a pathogenic role in the development of paraneoplastic iRMD associated with thymoma.
NEUROLOGY-NEUROIMMUNOLOGY & NEUROINFLAMMATION
(2023)
Article
Biology
Melanie Bodnar-Wachte, Anne-Laure Huber, Julie Gorry, Sabine Hacot, Delphine Burlet, Laetitia Gerossier, Baptiste Guey, Nadege Goutagny, Birke Bartosch, Elise Ballot, Julie Lecuelle, Caroline Truntzer, Francois Ghiringhelli, Benedicte F. Py, Yohann Coute, Annabelle Ballesta, Sylvie Lantuejoul, Janet Hall, Agnes Tissier, Virginie Petrilli
Summary: NLRP3 interacts with ATM kinase to control the activation of the DNA damage response, independent of its inflammasome activity. Down-regulated expression of NLRP3 in lung and breast cancers is associated with better patient overall survival. This discovery reveals a novel non-immune function for NLRP3 in maintaining genome integrity.
LIFE SCIENCE ALLIANCE
(2023)
Article
Microbiology
Mariane Pivard, Isabelle Caldelari, Virginie Brun, Delphine Croisier, Michel Jaquinod, Nelson Anzala, Benoit Gilquin, Chloe Teixeira, Yvonne Benito, Florence Couzon, Pascale Romby, Karen Moreau, Francois Vandenesch
Summary: The study found that there are significant variations in the production of Staphylococcus aureus HlgCB protein in patients with pneumonia, as well as inconsistent levels of HlgC and HlgB in certain strains. Analysis of clinical isolates and reference strains revealed that the levels of HlgCB and the HlgC/HlgB ratio depend on the activity of the hlgC promoter, mRNA processing, and translation. Additionally, a single nucleotide polymorphism (SNP) in the 5' untranslated region (UTR) of hlgCB mRNA was found to inhibit the translation of HlgC, leading to a decrease in HlgCB levels.
MICROBIOLOGY SPECTRUM
(2023)
Article
Developmental Biology
Valerie J. Robert, Matthieu Caron, Loic Gely, Annie Adrait, Victoria Pakulska, Yohann Coute, Manon Chevalier, Christian G. Riedel, Cecile Bedet, Francesca Palladino
Summary: The transcriptional co-regulator SIN3 plays a crucial role in regulating the germline transcriptome in Caenorhabditis elegans. Loss of SIN3 leads to maternal-effect sterility and de-silencing of X-linked genes. Different SIN3 complexes are identified, and SIN3 is found to be involved in silencing the X chromosome.
Review
Biochemical Research Methods
Thomas Burger
Summary: In the field of discovery proteomics, it is appealing to simultaneously test for the differential abundance of hundreds or thousands of features, but specific statistical safeguards, such as controlling for the false discovery rate (FDR), are necessary. The one-way analysis of variance (ANOVA) framework is commonly used when considering multiple biological conditions, and post-hoc tests (PHTs) are used for refining the initial differential abundance landscape. However, the interactions between FDR control procedures and PHTs are complex and require careful consideration.
Article
Virology
Federico Marziali, Yuxin Song, Xuan-Nhi Nguyen, Lucid Belmudes, Julien Burlaud-Gaillard, Philippe Roingeard, Yohann Coute, Andrea Cimarelli
Summary: This study identified NDFIP2 protein adaptor as an important regulator of IFITM3 levels and showed that NDFIP2 recruits IFITM3 and NEDD4 and mediates their distribution in lysosomal vesicles, thereby regulating the accumulation of IFITM3. The study reveals a novel and potentially dynamic layer of regulation of IFITM3.
Article
Multidisciplinary Sciences
Domenico Iuso, Isabel Garcia-Saez, Yohann Coute, Yoshiki Yamaryo-Botte, Elisabetta Boeri Erba, Annie Adrait, Nour Zeaiter, Malgorzata Tokarska-Schlattner, Zuzana Macek Jilkova, Faycal Boussouar, Sophie Barral, Luca Signor, Karine Couturier, Azadeh Hajmirza, Florent Chuffart, Ekaterina Bourova-Flin, Anne-Laure Vitte, Lisa Bargier, Denis Puthier, Thomas Decaens, Sophie Rousseaux, Cyrille Botte, Uwe Schlattner, Carlo Petosa, Saadi Khochbin
Summary: Nucleoside diphosphate kinases (NME1/2) regulate nucleotide homeostasis and bind to CoA. NME2 mediates gene transcription response in liver cells under high-fat diet, repressing fatty acid accumulation and activating protective gene expression program.
