Review
Biochemistry & Molecular Biology
Shigeyuki Kawai, Wataru Hashimoto
Summary: This article reviews the roles of 4-Deoxy-l-erythro-5-hexoseulose uronate (DEH), DEH reductase, and alginate lyase in the metabolism of alginate, a valuable carbon source derived from brown macroalgae for biorefinery applications. The understanding of DEH and DEH reductase is summarized, including their formation, structure, reactivity, molecular identification, classification, function, and significance in biorefinery. This knowledge is expected to contribute to the practical utilization of alginate for biorefinery.
Article
Biochemistry & Molecular Biology
Yuzuki Tanaka, Yoshihiro Murase, Toshiyuki Shibata, Reiji Tanaka, Tetsushi Mori, Hideo Miyake
Summary: In this study, recombinant enzymes were prepared and immobilized to efficiently convert alginate into DEH. The immobilized enzymes showed high activity even after repeated use, resulting in a relatively high DEH yield.
Article
Biology
Ryuji Nishiyama, Takao Ojima, Yuki Ohnishi, Yasuhiro Kumaki, Tomoyasu Aizawa, Akira Inoue
Summary: Nishiyama et al. described a biochemical pathway for converting DEHU to alpha-ketoglutarate in the alginate-assimilating marine bacterium Flavobacterium sp. strain UMI-01. They identified two key enzymes involved in the catabolism of KDGR, revealing the dual pathways of DEHU metabolism involving reduction or oxidation by FlRed.
COMMUNICATIONS BIOLOGY
(2021)
Article
Polymer Science
Ragaa A. Hamouda, Asmaa S. Salman, Asrar A. Alharbi, Reem Hasaballah Alhasani, Maha M. Elshamy
Summary: This study evaluated the protective role of Zinc oxide alginate-nanocomposites (ZnO-Alg/NCMs) against MMC-induced genotoxicity in mice. The ZnO-Alg/NCMs showed significant protection against MMC-induced genotoxic disturbances, demonstrating promising potential as a candidate for protecting against MMC-induced genotoxicity.
Article
Fisheries
Miu Sakagami, Yuki Ohnishi, Yasuhiro Kumaki, Tomoyasu Aizawa, Akira Inoue
Summary: This study reports the first MC5E enzymatic activity in brown algae of the family Chordariaceae, and identifies a novel MC5E candidate protein, CoC5-1, from the brown alga Cladosiphon okamuranus. CoC5-1 possesses MC5E activity and exhibits higher thermal stability compared to SjC5-VI.
Article
Chemistry, Multidisciplinary
Jiao Chen, Jiali Cai, Feng Sha, Wenjun Sun, Xilei Lyu, Yonghui Chang, Fei Cao, Lili Zhao, Hongli Wu, Pingkai Ouyang
Summary: A new chemo-enzymatic strategy for generating furan-2,5-dicarboxylic acid (FDCA) precursors from sodium gluconate via bio-oxidation and dehydration is presented. The specific product 5-keto-d-gluconic acid (5KGA), a stable intermediate, was obtained in 99% yield. The yield of the FDCA precursor n-butyl-5-formyl-2-furancarboxylate (nBu-FFCA) reached 77.9% via the dehydration and esterification of 5KGA.
Article
Fisheries
Fang Lu, Meili Xin, Liuqing Tang, Dongmei Zhan, Gang Ding, Haiyi Wu
Summary: This study found that diluted seawater concentration significantly affects the growth, antioxidation capacity, and accumulation of bioactive compounds of Sargassum muticum, providing valuable data for the development and management of the cultivation of this brown algae species.
AQUACULTURE RESEARCH
(2022)
Article
Microbiology
Tristan Barbeyron, Nolwen Le Duff, Eric Duchaud, Francois Thomas
Summary: Strain LLG6346-3.1T, isolated from the brown alga Ericaria zosteroides collected from the Mediterranean Sea, was characterized as a novel species of the genus Zobellia. LLG6346-3.1T exhibited distinct phenotypic, phylogenetic, and genomic characteristics from other Zobellia species. The name Zobellia alginiliquefaciens sp. nov. is proposed for this bacterium.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Olesya S. Malyarenko, Roza V. Usoltseva, Artem S. Silchenko, Anastasiya O. Zueva, Svetlana P. Ermakova
Summary: Melanoma, the most aggressive and treatment-resistant form of skin cancer, can be targeted by metabolic modifiers such as fucoidan. The combination of fucoidan with 2-deoxy-D-glucose (2-DG) effectively inhibits the proliferation and colony formation of melanoma cells. This study sheds light on the mechanism of action and potential therapeutic use of fucoidan and its derivative in combination with 2-DG for melanoma treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Tetsu Shimizu, Akira Nakamura
Summary: A previously unidentified protein (TTHB078) from Thermus thermophilus HB8 exhibits a novel reductase activity on KDG. This enzyme, named KdgG, uses NADH and NADPH as electron donors and catalyzes the reversible reduction of KDG to form 3-deoxy-d-mannonate.
Article
Biotechnology & Applied Microbiology
Jianhua Li, Fanglin Xu, Dongni Ji, Chenfei Tian, Yuwei Sun, Ishmael Mutanda, Yuhong Ren, Yong Wang
Summary: 5-deoxy(iso)flavonoids are important compounds in plants with potential applications in pharmaceuticals, nutraceuticals, and food additives. By utilizing specific enzymes and protein-protein interaction strategies, production of 5-deoxy(iso)flavonoids can be improved and product quality can be optimized.
METABOLIC ENGINEERING COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Donn G. Wishka, Omar D. Lopez, Vladimir F. Rudchenko, Guangfei Huang, Robert Bahde, Vineet Kumar, Sergiy M. Denysenko, Lianhao Zhang, Mianji Zhang, Beverly A. Teicher, Joel Morris
Summary: This article describes the modification and optimization of previous methods to enable the large-scale synthesis of benzyl substituted 2-deoxy-1,4-dithio-D-erythro-pentofuranosides, as well as the development of reaction conditions for beta-selective glycosylation reactions with N4-benzoylcytosine and 5-aza-cytosine for practical multi-gram syntheses of clinical candidates. These synthetic developments have facilitated the preclinical and early clinical development of important anticancer agents at the National Cancer Institute.
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
(2021)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)