Review
Cell Biology
Aleksei Innokentev, Tomotake Kanki
Summary: Mitophagy is a selective autophagy process that degrades damaged mitochondria to maintain mitochondrial homeostasis. Recent studies have elucidated the molecular mechanisms and physiological roles of mitophagy in yeast and mammalian cells.
Review
Biochemistry & Molecular Biology
Yang Liu, Koji Okamoto
Summary: Mitophagy is an evolutionarily conserved process that selectively degrades mitochondria via autophagy, and is intricately regulated through transcriptional induction and post-translational modifications of Atg32 in yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Review
Cell Biology
Ingrid Bhatia-Kissova, Nadine Camougrand
Summary: Mitophagy, the selective degradation of mitochondria by autophagy, is crucial for cellular homeostasis. Studies on yeasts have revealed important insights into the molecular mechanisms and role of the Atg32 receptor in mitophagy, showing distinct physiological roles compared to mammals. Different tools for studying mitophagy are also discussed in this review.
Article
Biochemistry & Molecular Biology
Ramona Schuster, Koji Okamoto
Summary: This review summarizes the mechanism of mitophagy in yeast and discusses its relationship with mitochondrial dynamics and the ubiquitin-proteasome system.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2022)
Article
Biochemistry & Molecular Biology
Mitsutaka Kubota, Koji Okamoto
Summary: The study demonstrates that the NatA complex promotes mitophagy through facilitating the phosphorylation of Atg32 independently of Atg32-Atg11 interactions. Loss of NatA leads to altered Atg32 phosphorylation, and overphosphorylation of Atg32 can partially restore mitophagy in NatA-deficient cells. These findings suggest that NatA-mediated protein N-terminal acetylation plays a role in Atg32 expression and phosphorylation to drive mitophagy.
JOURNAL OF BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Mitsutaka Kubota, Koji Okamoto
Summary: This passage describes the importance of mitophagy and the role of the N-terminal acetyltransferase A complex in mitophagy in yeast cells, promoting mitophagy through phosphorylation of Atg32. Furthermore, it suggests that NatA functions to drive mitophagy through the expression and phosphorylation of Atg32.
JOURNAL OF BIOCHEMISTRY
(2021)
Article
Biology
Panagiota Kolitsida, Vladimir Nolic, Jianwen Zhou, Michael Stumpe, Natalie M. Niemi, Jorn Dengjel, Hagai Abeliovich
Summary: The mitophagic degradation of mitochondrial matrix proteins in Saccharomyces cerevisiae is selectively regulated by the matrix kinases Pkp1 and Pkp2, which are in turn regulated by the phosphatase Aup1/Ptc6. These proteins also regulate the phosphorylation status and catalytic activity of the pyruvate dehydrogenase complex, which is critical for mitochondrial metabolism. The pyruvate dehydrogenase complex has been found to have a novel function in regulating mitophagic selectivity independent of its enzymatic activity.
LIFE SCIENCE ALLIANCE
(2023)
Editorial Material
Cell Biology
Tomoyuki Fukuda, Kentaro Furukawa, Tatsuro Maruyama, Nobuo N. Noda, Tomotake Kanki
Summary: Mitophagy is a selective form of autophagy that targets dysfunctional or superfluous mitochondria for degradation. Our recent study has identified Atg44 as a mitochondrial fission factor that generates mitochondrial fragments suitable for phagophore engulfment. We propose the term mitofissin to refer to Atg44 and its homologous proteins that might participate in diverse cellular processes.
Article
Cell Biology
Zulin Wu, Haiqian Xu, Junze Liu, Fan Zhou, Yongheng Liang
Summary: The study reveals that the ESCRT-III complex in yeast plays a role in mediating macromitophagy induced by nitrogen starvation. By interacting with components of the autophagosome and mitophagy receptor, the ESCRT-III complex contributes to the delivery and degradation of mitochondria. The results suggest a new function for ESCRT-III in regulating mitochondrial clearance in yeast.
Article
Biochemistry & Molecular Biology
James H. Schofield, Zachary T. Schafer
Summary: The relationship between mitophagy and ROS production is complex and not fully understood. This review discusses mtROS generation and their detrimental effects on cellular viability, along with the cellular defense mechanisms against oxidative stress. Furthermore, the prominent mechanisms governing mitophagy induction that bear on oxidative stress are explored.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Editorial Material
Cell Biology
Tomoyuki Fukuda, Tomotake Kanki
Summary: Mitophagy is a selective form of autophagy that degrades damaged or unnecessary mitochondria through double-membranous structures called phagophores. Atg43 has been identified as a mitophagy receptor in the fission yeast Schizosaccharomyces pombe, playing a role in stabilizing phagophore expansion on mitochondria by interacting with Atg8. This suggests that Atg43 may have acquired its mitophagic function through convergent evolution, as it shares no sequence similarity with mitophagy receptors in other organisms.
Review
Cell Biology
Ting Zhang, Qian Liu, Weihua Gao, Sheikh Arslan Sehgal, Hao Wu
Summary: Mitochondria play crucial roles in cellular metabolism and regulated cell death, with mitophagy serving as a key mechanism for maintaining cellular homeostasis. Mitophagy is regulated by various endogenous metabolites and is essential for the bidirectional interplay between mitophagy and cellular metabolisms.
Article
Multidisciplinary Sciences
Mack B. Reynolds, Hanna S. Hong, Britton C. Michmerhuizen, Anna-Lisa E. Lawrence, Li Zhang, Jason S. Knight, Costas A. Lyssiotis, Basel H. Abuaita, Mary X. O'Riordan
Summary: Macrophage metabolic plasticity allows the redirection of electron transport for inflammation and host defense. Inflammatory activation leads to disassembly of Complex II and loss of succinate dehydrogenase subunit B through sequestration and selective mitophagy. The regulatory role of cardiolipin in coordinating Complex II function and inflammatory metabolic remodeling is demonstrated.
Article
Chemistry, Multidisciplinary
Lina Wang, Taotao Qiang, Longfang Ren, Fei Cheng, Wei Hu, Renyu Qu
Summary: Diabetic ulcers (DUs) have become a challenging disease due to unclear healing mechanisms, with the polarization state of macrophages and autophagy playing crucial roles in the delayed healing process.
Review
Pharmacology & Pharmacy
Alexander V. Blagov, Andrey G. Goncharov, Olga O. Babich, Viktoriya V. Larina, Alexander N. Orekhov, Alexandra A. Melnichenko
Summary: This review discusses the use of mitophagy activators as a class of drug compounds for the treatment of Parkinson's disease, and explores the impact of mutations in Pink1 and Parkin enzymes on mitophagy.
Editorial Material
Cell Biology
Tomoyuki Fukuda, Tomotake Kanki
Summary: Mitophagy is a selective form of autophagy that degrades damaged or unnecessary mitochondria through double-membranous structures called phagophores. Atg43 has been identified as a mitophagy receptor in the fission yeast Schizosaccharomyces pombe, playing a role in stabilizing phagophore expansion on mitochondria by interacting with Atg8. This suggests that Atg43 may have acquired its mitophagic function through convergent evolution, as it shares no sequence similarity with mitophagy receptors in other organisms.
Article
Biochemistry & Molecular Biology
Isshin Shiiba, Keisuke Takeda, Shun Nagashima, Naoki Ito, Takeshi Tokuyama, Shun-Ichi Yamashita, Tomotake Kanki, Toru Komatsu, Yasuteru Urano, Yuuta Fujikawa, Ryoko Inatome, Shigeru Yanagi
Summary: MITOL regulates Parkin-mediated cell death by promoting ubiquitination of Parkin at lysine 220 residue, leading to its proteasomal degradation and controlling mitophagy. Deletion of MITOL results in accumulation of active phosphorylated Parkin in the ER, causing FKBP38 degradation and increased cell death.
Article
Biology
Tomoyuki Fukuda, Fajar Sofyantoro, Yen Teng Tai, Kim Hou Chia, Takato Matsuda, Takaaki Murase, Yuichi Morozumi, Hisashi Tatebe, Tomotake Kanki, Kazuhiro Shiozaki
Summary: The GATOR2 subunit Sea3 unexpectedly attenuates TORC1 activity by being physically and functionally proximal to GATOR1 in fission yeast. The GATOR complex in fission yeast is dispensable for TORC1 regulation in response to amino acid starvation, as cells instead activate the Gcn2 pathway to inhibit TORC1 and induce autophagy.
Article
Cell Biology
Shun-Ichi Yamashita, Masanao Kyuuma, Keiichi Inoue, Yuki Hata, Ryu Kawada, Masaki Yamabi, Yasuyuki Fujii, Junko Sakagami, Tomoyuki Fukuda, Kentaro Furukawa, Satoshi Tsukamoto, Tomotake Kanki
Summary: The study found that muscle disuse enhances mitophagy activity and the production of reactive oxygen species in atrophic skeletal muscles, suggesting a potential therapeutic target for disuse-induced muscle atrophy.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Endocrinology & Metabolism
Kyota Aoyagi, Shun-ichi Yamashita, Yoshihiro Akimoto, Chiyono Nishiwaki, Yoko Nakamichi, Haruhide Udagawa, Manabu Abe, Kenji Sakimura, Tomotake Kanki, Mica Ohara-Imaizumi
Summary: This study established a new method for evaluating Mitophagy in beta cells using CMMR mice. It was found that metabolic stress induced by a high-fat diet led to aberrant accumulation of dysfunctional mitochondria, overwhelming the capacity of Mitophagy and impairing mitochondrial function and insulin secretion.
Review
Immunology
Rajendra Karki, Thirumala-Devi Kanneganti
Summary: ADAR1 and ZBP1 are two mammalian proteins with Z alpha domains that bind to nucleic acids. They play crucial roles in regulating various biological processes. Recent studies have expanded our understanding of their functions, establishing their fundamental regulation of innate immune responses. This review discusses their roles in innate immune responses in development and disease.
TRENDS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Tomoyuki Fukuda, Tetsu Saigusa, Kentaro Furukawa, Keiichi Inoue, Shun-ichi Yamashita, Tomotake Kanki
Summary: The endoplasmic reticulum (ER) undergoes selective autophagy called reticulophagy or ER-phagy. Reticulon- and receptor expression enhancing protein (REEP)-like ER-shaping proteins, such as budding yeast Atg40, act as reticulophagy receptors to stabilize the phagophore on the ER by interacting with phagophore-conjugated Atg8. Hva22, a REEP family protein in fission yeast, promotes reticulophagy without Atg8-binding capacity and its role can be replaced by expressing Atg40 independently of its Atg8-binding ability.
Review
Immunology
Wen Chen, Jessica M. M. Gullett, Rebecca E. E. Tweedell, Thirumala-Devi Kanneganti
Summary: Regulated cell death (RCD) is important for host survival during pathogen invasion and cellular disturbances. This review focuses on the newly discovered RCD pathway called PANoptosis, which is an inflammatory cell death pathway regulated by PANoptosome complexes. The article discusses the molecular components of PANoptosomes, their assembly, activation, regulation, and their link to diseases.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Kanako Watanabe, Tomoichiro Oka, Hirotaka Takagi, Sergei Anisimov, Shun-ichi Yamashita, Yoshinori Katsuragi, Masahiko Takahashi, Masaya Higuchi, Tomotake Kanki, Akihiko Saitoh, Masahiro Fujii
Summary: The host factor MYADM is identified as essential for PeV-A infection and is involved in virus entry into host cells in vitro. This finding advances our understanding of the pathogenesis of PeV-A.
NATURE COMMUNICATIONS
(2023)
Editorial Material
Cell Biology
Tomoyuki Fukuda, Kentaro Furukawa, Tatsuro Maruyama, Nobuo N. Noda, Tomotake Kanki
Summary: Mitophagy is a selective form of autophagy that targets dysfunctional or superfluous mitochondria for degradation. Our recent study has identified Atg44 as a mitochondrial fission factor that generates mitochondrial fragments suitable for phagophore engulfment. We propose the term mitofissin to refer to Atg44 and its homologous proteins that might participate in diverse cellular processes.
Article
Multidisciplinary Sciences
Tatsuya Shioda, Ittetsu Takahashi, Kensuke Ikenaka, Naonobu Fujita, Tomotake Kanki, Toshihiko Oka, Hideki Mochizuki, Adam Antebi, Tamotsu Yoshimori, Shuhei Nakamura
Summary: Accumulating evidence suggests that the molecular network involving neuronal MML-1 function is crucial for regulating systemic aging. This study demonstrates that MML-1 and its heterodimer partners play a primary role in neurons to extend longevity in germlineless animals, acting via downstream cascades that are distinct from HLH-30. The MML-1-GLT-5 axis in neurons is critical for maintaining proteostasis and reducing oxidative stress in long-lived animals through autophagy and peroxidase MLT-7, respectively.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Yen Teng Tai, Tomoyuki Fukuda, Yuichi Morozumi, Hayato Hirai, Arisa H. Oda, Yoshiaki Kamada, Yutaka Akikusa, Tomotake Kanki, Kunihiro Ohta, Kazuhiro Shiozaki
Summary: TORC1 is activated in response to nutrient availability and growth factors, promoting cellular anabolism and proliferation. This study revealed that TORC1 phosphorylates and protects the transcription factor Sfp1, which then positively regulates ribosome production genes together with Ifh1 and Fhl1. The transcriptional regulation of ribosome biosynthesis genes by Sfp1, Ifh1, and Fhl1 is one of the key pathways through which nutrient-activated TORC1 promotes cell proliferation.
MOLECULAR AND CELLULAR BIOLOGY
(2023)
