期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 698, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2020.108722
关键词
beta-Catenin; TNBC; Serine-tyrosine phosphorylation; Wnt signaling pathway; Molecular dynamics simulation
This study investigates the role of beta-Catenin in TNBC through molecular dynamics, revealing that phosphorylation induces structural and dynamic changes in the protein, leading to significant secondary structure transitions.
beta-Catenin, a key transcriptional factor involved in the canonical Wnt signaling pathway, is regulated by a cascade of phosphorylations and plays a major role in the progression of triple-negative breast cancer (TNBC). However, the phosphorylation induced conformational changes in a beta-Catenin is still poorly understood. Hence, we adopted a conventional molecular dynamics approach to study phosphorylations present in a sequence motif Ser(552 675) and Tyr(670) of the beta-Catenin domain and analyzed in terms of structural transitions, bond formation, and folding-misfolding conformations. Our results unveil the beta-Catenin linear motif 549-555 (RRTSMGG) of armadillo repeats domain prefers order to disorder state. In contrast, helix C associated with 670-678 (YKKRLSVEL) motif prefers disorder to order upon phosphorylation of Ser(552-675) and Tyr(670). In addition, the crucial secondary structural transition from alpha-helix to coil induced by phospho Ser(552) and phospho Tyr(670) of beta-Catenin ARM domain connecting helix C modifies conformational diversity and binding affinities of the complex interaction in functional regulation significantly. Moreover, the post phosphorylation disrupted the hydrogen bond interactions (Ser552-Arg549, Arg550-Asp546 and Ser675-Lys672) and abolished the residual alliance with hydrophobic interactions (Tyr670-Leu674) that easily interrupt in secondary structure packing as well as folding conformations connecting ARM and helix C (R10, 12 & R1C) compared to unphosphorylation. Our integrated computational analysis may help in shedding light on understanding the induced folding and unfolding pattern due to motif phosphorylations. Overall, our results provide an atomistic structural description of the way phosphorylation facilitates conformational and dynamic changes in beta-Catenin, a fundamental molecular switch mechanism in triple-negative breast cancer pathogenesis.
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