Article
Engineering, Biomedical
Marisela Rodriguez-Salvador, Itzel Fox-Miranda, Baruc Emet Perez-Benitez, Jose Ricardo Lopez-Robles
Summary: Tissue spheroids are an innovative solution in tissue engineering and regenerative medicine, capable of mimicking the complex composition of native tissues. There is a need to assess the research dynamics in this field, especially regarding the use of tissue spheroids as building blocks. In this study, a scientometric analysis was conducted to characterize the evolution of research using tissue spheroids as building blocks to generate tissue constructs. The analysis provides valuable insights for research and development strategies in new technologies like tissue spheroids.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2022)
Review
Engineering, Biomedical
Dishary Banerjee, Yogendra Pratap Singh, Pallab Datta, Veli Ozbolat, Aaron O'Donnell, Miji Yeo, Ibrahim T. Ozbolat
Summary: Biofabricated tissues have versatile applications in tissue engineering and regenerative medicine, as well as disease modeling and drug development and screening. Spheroid bioprinting has emerged as a crucial tool for assembling and organizing multiple types of cells into physiologically and histologically relevant tissues.
Article
Biotechnology & Applied Microbiology
Jessica Nulty, Ross Burdis, Daniel J. Kelly
Summary: Bone tissue engineering has potential to revolutionize treatment of challenging musculoskeletal pathologies. Developing tissue-engineered constructs mimicking early stages of tissue development can address issues of poor vascularization in traditional strategies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Dong Nyoung Heo, Bugra Ayan, Madhuri Dey, Dishary Banerjee, Hwabok Wee, Gregory S. Lewis, Ibrahim T. Ozbolat
Summary: This study presents a viable approach for 3D bioprinting of complex-shaped geometries using spheroids as building blocks, which can be used for various applications including tissue engineering, organ-on-a-chip and microfluidic devices, drug screening, and disease modeling. The use of spheroids as building blocks allowed for the fabrication of scaffold-free bone tissue constructs with enhanced osteogenic differentiation and cell viability. Bioprinted bone tissues showed high expression of osteogenic and endothelial-specific gene factors, indicating their potential for clinical translation.
Review
Engineering, Biomedical
Pei Zhuang, Yi-Hua Chiang, Maria Serafim Fernanda, Mei He
Summary: Although cancer remains a leading cause of mortality worldwide, efforts in anticancer therapeutics are hindered by the lack of robust prediction models. Multicellular tumor spheroids, a popular three-dimensional culture model for avascular tumors, have limitations in controlling cellular and structural organization. 3D bioprinting, with its spatial control, scalability, and reproducibility, holds promise for generating more faithful tumor models and advancing our understanding of tumor progression.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2021)
Review
Engineering, Biomedical
Ming Li, Xueer Song, Sha Jin, Kaiming Ye
Summary: This review highlights the use of three-dimensional tumor models in cancer pathology studies and drug discovery, which can simulate the complex human tumor microenvironment. Various biofabrication technologies, including 3D bioprinting techniques, are discussed for characterizing tumor progression and response to treatment.
BIO-DESIGN AND MANUFACTURING
(2021)
Article
Engineering, Biomedical
David J. Bustamante, Elijah J. Basile, Brady M. Hildreth, Nathan W. Browning, S. Alexander Jensen, Leni Moldovan, Horia Petrache, Nicanor Moldovan
Summary: The availability of nutrients like glucose in tumor spheroid models greatly influences their structure and cell fusion, with low glucose concentrations causing sudden collapse and high concentrations leading to increased cell mixing, oscillations between cell growth and dormancy, and structural instability. The competition for glucose in a tumor microenvironment can result in tumor disappearance, stability, or expansion, while the invasion of individual tumor cells is strongly dependent on glucose availability.
Review
Engineering, Biomedical
Haoyu Li, Huixing Zhou, Chongwen Xu, Yen Wei, Xiuying Tang
Summary: This review provides an overview of the mechanisms, advantages, and limitations of scaffold-based and scaffold-free strategies in tissue engineering. It compares the strengths and weaknesses of these two strategies and discusses the challenges in their future development. The review concludes that integrating scaffold-based and scaffold-free strategies can overcome the problems in biofabrication.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Engineering, Biomedical
Pierluca Pitacco, Joanna M. Sadowska, Fergal J. O'Brien, Daniel J. Kelly
Summary: This study demonstrates the use of 3D bioprinting to engineer mechanically-reinforced cartilaginous templates for large bone defect regeneration. The study found that hypertrophic grafts engineered using TGF-beta 3 and BMP-2 supported higher levels of blood vessel infiltration and accelerated bone regeneration.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Xavier Barcelo, Kian F. Eichholz, Ines F. Goncalves, Orquidea Garcia, Daniel J. Kelly
Summary: This study demonstrates a biofabrication strategy using inkjet bioprinting to engineer fibrocartilage tissues with user-defined collagen architectures. The scaffold architecture was found to influence the spatial organization of the collagen network, and higher aspect ratios supported the formation of structurally anisotropic tissues. This technology could potentially provide new treatment options for damaged and diseased meniscal tissue.
ACTA BIOMATERIALIA
(2023)
Article
Biotechnology & Applied Microbiology
Selcan Guler, Kian Eichholz, Farhad Chariyev-Prinz, Pierluca Pitacco, Halil Murat Aydin, Daniel J. Kelly, Ibrahim Vargel
Summary: In this study, a biocompatible and bioactive scaffold for bone tissue engineering (BTE) was fabricated using poly(glycerol sebacate) (PGS) and decellularized bone extracellular matrix (deB ECM). The optimized scaffold showed improved initial cell attachment, osteogenesis, mechanical strength, degradation rate, pH change, and wettability. The small-pore-sized and 28% (w/w) bone-inserted scaffolds demonstrated better osteogenic differentiation of mesenchymal stem cells (MSCs).
BIOENGINEERING-BASEL
(2023)
Article
Engineering, Biomedical
Lianne C. Shanley, Olwyn R. Mahon, Sinead A. O'Rourke, Nuno G. B. Neto, Michael G. Monaghan, Daniel J. Kelly, Aisling Dunne
Summary: Recent studies have shown that immune cells undergo metabolic reprogramming upon activation, and exploiting this process has therapeutic potential. This study demonstrates for the first time that the size of hydroxyapatite particles can impact macrophage metabolism, with micron-sized particles promoting a pro-inflammatory phenotype through glycolysis. This highlights immunometabolism as a targetable aspect of the immune response for therapeutic benefit and informs intelligent biomaterial design.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Fiona E. Freeman, Pere Dosta, Lianne C. Shanley, Natalia Ramirez Tamez, Cristobal Riojas J. Javelly, Olwyn R. Mahon, Daniel J. Kelly, Natalie Artzi
Summary: This study investigates the potential of miR-29b to suppress osteosarcoma tumors and normalize bone dysregulation caused by the tumor. The delivery of miR-29b along with systemic chemotherapy significantly decreases tumor burden, increases mouse survival, and decreases osteolysis, thereby normalizing bone homeostasis.
ADVANCED MATERIALS
(2023)
Article
Biophysics
Farhad Chariyev-Prinz, Alex Szojka, Nuno Neto, Ross Burdis, Michael G. Monaghan, Daniel J. Kelly
Summary: Mechanical stimulation can modulate the chondrogenic differentiation of stem/progenitor cells, but its effect on functional articular cartilage engineering using human mesenchymal stem cells (hMSCs) may not be necessary if other culture conditions have been optimized. This study investigated the impact of cell seeding densities and fibrin concentrations on chondrogenesis of hMSCs and found that high cell densities were more supportive of sGAG deposition, while lower fibrin concentrations promoted more robust chondrogenesis. Mechanical stimulation had limited effect on cartilage-specific matrix production, except when hMSCs were first primed with TGF-beta 3. These findings suggest that mechanical stimulation may not be essential for engineering functional articular cartilage grafts, but can be used to understand tissue response to mechanical loading in vivo.
JOURNAL OF BIOMECHANICS
(2023)
Article
Engineering, Biomedical
Ian T. Whelan, Ross Burdis, Somayeh Shahreza, Emad Moeendarbary, David A. Hoey, Daniel J. Kelly
Summary: Endochondral ossification (EO) is a critical biological process for bone development and healing. However, understanding of this process is limited, preventing effective treatment of dysregulated EO. The lack of predictive in vitro models is a key factor, and microphysiological systems offer an advanced platform for research. In this study, a microphysiological model of vascular invasion into developing/regenerating bone was developed, mimicking the process of EO. This model successfully replicated key events in EO and could be used for further research and drug testing.
Editorial Material
Engineering, Biomedical
Daniel J. Kelly, Marcy Zenobi-Wong
Article
Engineering, Biomedical
Bin Wang, Xavier Barcelo, Stanislas Von Euw, Daniel J. Kelly
Summary: Decellularized extracellular matrix (dECM) is a promising biomaterial for tissue engineering and regenerative medicine. This study successfully produced highly concentrated dECM inks with improved mechanical properties through different solubilization and decellularization methods. The 3D printing of these dECM inks at higher pH levels enabled the fabrication of anatomically defined meniscal implants with compressive mechanical properties similar to native tissue. These findings demonstrate the potential of 3D printing of highly concentrated dECM inks for meniscal tissue regeneration and have applications in tissue engineering and surgical planning.
MATERIALS TODAY BIO
(2023)
Article
Biochemistry & Molecular Biology
Piotr Gierlich, Claire Donohoe, Kevin Behan, Daniel J. Kelly, Mathias O. Senge, Ligia C. Gomes-da-Silva
Summary: A pH-sensitive hydrogel formulation was developed for intratumoral injection, enabling sustained release of photosensitizers and achieving optimal therapeutic outcomes through multiple light exposures.
Article
Engineering, Biomedical
Ross Burdis, Xavier Barcelo Gallostra, Daniel J. Kelly
Summary: Scaffold-free tissue engineering can generate more hyaline-like cartilage tissue compared to single-cell scaffold-free approaches. Temporal enzymatic treatment can enhance microtissue fusion and tissue remodeling, leading to the formation of a denser and more biomimetic zonal collagen network. This study demonstrates that using biological building blocks can be improved by using temporal enzymatic treatment during graft development.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Cell & Tissue Engineering
Farhad Chariyev-Prinz, Nuno Neto, Michael G. Monaghan, Daniel J. Kelly
Summary: It is beneficial to apply hydrostatic pressure for MSC chondrogenesis, but the timing of application is crucial for its impact on cell fate and tissue development. Understanding the response of engineered cartilage to hydrostatic pressure can determine the optimal time for in vivo implantation. This study examined the chondrogenic maturation of hMSCs over time and found that hydrostatic pressure enhanced chondrogenic gene ratios after 35 days of priming.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2023)
Article
Cell & Tissue Engineering
M. Joyce, T. Hodgkinson, M. Lemoine, A. Gonzalez-Vazquez, D. J. Kelly, F. J. O'Brien
Summary: Collagen hyaluronic-acid (CHyA) matrix reinforced with bioabsorbable 3D-printed polycaprolactone (PCL) scaffold shows promise for treating large-area chondral defects that currently lack effective treatment options, due to its ability to support physiological loads and enhance chondroinductive potential.
EUROPEAN CELLS & MATERIALS
(2023)
Article
Engineering, Biomedical
Kian F. Eichholz, Pierluca Pitacco, Ross Burdis, Farhad Chariyev-Prinz, Xavier Barcelo, Brooke Tornifoglio, Ryan Paetzold, Orquidea Garcia, Daniel J. Kelly
Summary: This study demonstrates the potential of integrating different additive manufacturing approaches, specifically MEW and FDM, for the development of regenerative biomaterials. By leveraging the advantages of these two methods, hybrid MEW-FDM constructs are able to achieve enhanced bone healing outcomes.
ADVANCED HEALTHCARE MATERIALS
(2023)