期刊
ACS CHEMICAL BIOLOGY
卷 16, 期 3, 页码 491-500出版社
AMER CHEMICAL SOC
DOI: 10.1021/acschembio.0c00840
关键词
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资金
- Zhangjiang National Innovation Demonstration Zone [ZJ2020-ZD-004]
- Zhejiang University special scientific research fund for COVID-19 prevention and control [2020XGZX011]
- Cultivation Project of Shanghai Major Infectious Disease Research Base [20dz2210500]
- National Key Research and Development Program of China [2018YFC1200100, 2018ZX10301403]
- emergency grants for prevention and control of SARS-CoV-2 of Ministry of Science and Technology [2020YFC0841400]
- Shanghai Municipal Key Clinical Specialty [shslczdzk02202]
- Shanghai Top-Priority Clinical Key Disciplines Construction Project [2017ZZ02014]
- Shanghai Shenkang Hospital Development Center Clinical Science and Technology Innovation Project [SHDC12018102]
- National Innovative Research Team of High-level Local Universities in Shanghai
- National Natural Science Foundation of China [91853106]
- Program for Guangdong Introducing Innovative and Enterpre-neurial Teams [2016ZT06Y337]
- Fundamental Research Funds for the Central Universities [19ykzd25]
- Key Laboratory of Emergency Prevention, Diagnosis and Treatment of Respiratory Infectious Diseases, Shanghai [20dz2261100]
- ShanghaiTech University COVID-19 Special Fund
- ShanghaiTech University Startup Fund [2019F0301-000-01]
The outbreak of novel coronavirus SARS-CoV-2 poses a global threat to public health, highlighting the urgent need for sensitive and specific detection methods. The CALIBURN platform offers rapid and accurate detection of SARS-CoV-2 and other viruses, with broad specimen compatibility.
The outbreak of novel coronavirus SARS-CoV-2 has caused a worldwide threat to public health. COVID-19 patients with SARS-CoV-2 infection can develop clinical symptoms that are often confused with the infections of other respiratory pathogens. Sensitive and specific detection of SARS-CoV-2 with the ability to discriminate from other viruses is urgently needed for COVID-19 diagnosis. Herein, we streamlined a highly efficient CRISPR-Cas12a-based nucleic acid detection platform, termed Cas12a-linked beam unlocking reaction (CALIBURN). We show that CALIBURN could detect SARS-CoV-2 and other coronaviruses and influenza viruses with little cross-reactivity. Importantly, CALIBURN allowed accurate diagnosis of dinical samples with extremely low viral loads, which is a major obstacle for the clinical applications of existing CRISPR diagnostic platforms. When tested on the specimens from SARS-CoV-2-positive and negative donors, CALIBURN exhibited 73.0% positive and 19.0% presumptive positive rates and 100% specificity. Moreover, unlike existing CRISPR detection methods that were mainly restricted to respiratory specimens, CALIBURN displayed consistent performance across both respiratory and nonrespiratory specimens, suggesting its broad specimen compatibility. Finally, using a mouse model of SARS-CoV-2 infection, we demonstrated that CALIBURN allowed detection of coexisting pathogens without cross-reactivity from a single tissue specimen. Our results suggest that CALIBURN can serve as a versatile platform for the diagnosis of COVID-19 and other respiratory infectious diseases.
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