Article
Pharmacology & Pharmacy
Nianan Luo, Jiangbin Li, Yafeng Chen, Yan Xu, Yu Wei, Jianguo Lu, Rui Dong
Summary: The study demonstrated the efficient delivery of CRISPR/dCas9-VP64 system into HSCs via exosomes derived from AML12 cells, leading to induction of HSCs into hepatocyte-like phenotype, thus providing a potential gene therapy strategy for hepatic fibrosis treatment.
Article
Biochemistry & Molecular Biology
Nasir Javaid, Thuong L. H. Pham, Sangdun Choi
Summary: Reversing the transcriptional status of genes using CRISPR/dCas9-based activators has proven to be useful for various purposes. Our study demonstrates that dCas9-VP192 is more effective than VP64-dCas9-VP64 in activating reference genes, resulting in higher mRNA and protein levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nanoscience & Nanotechnology
Nora Igaz, Peter Belteky, David Kovacs, Csaba Papp, Andrea Ronavari, Diana Szabo, Attila Gacser, Zoltan Konya, Monika Kiricsi
Summary: The efficient delivery of Rhodamine 123 and Mitomycin C to multidrug-resistant cells via mesoporous silica nanoparticles (MSNs), resulting in increased intracellular drug concentration and enhanced cytotoxic effects, demonstrates the enormous potential of MSNs in overcoming multidrug-resistant cancer.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Engineering, Biomedical
Yushuang Shang, Jinsong Du, Baoru Wang, Pengfei Lu, Yongai Zhao, Jiang Yuan
Summary: Designing a drug delivery system that can respond to the tumor microenvironment is essential for enhancing the effectiveness of antitumor drugs and reducing side effects. This study demonstrates that DOX-loaded MSNs-NH2@Keratin exhibits pH and glutathione dual responsiveness under the tumor microenvironment, effectively inhibiting tumor cell growth and maintaining stable nanoparticle size in serum.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2022)
Article
Biochemical Research Methods
Mohammad Mijanur Rahman, Trygve O. Tollefsbol
Summary: Cancer therapeutics is constantly evolving, with CRISPR-dCas9 emerging as a promising precision cancer treatment option that can modify cancer epigenetic features. Challenges such as off-target effects and lack of efficient delivery systems need to be addressed when targeting cancer with sgRNA-dCas9.
Article
Biochemistry & Molecular Biology
Nianan Luo, Wenjun Zhong, Jiangbin Li, Jianguo Lu, Rui Dong
Summary: Hepatic fibrosis is a pathological reaction in the liver caused by various pathogenic factors, and there is currently no effective specific treatment. The CRISPR/dCas9 system, a new generation gene editing technology, holds promise for hepatic fibrosis therapy by targeting liver fibrosis effector cells, but faces challenges as well.
MOLECULAR BIOLOGY REPORTS
(2022)
Review
Plant Sciences
Chou Khai Soong Karlson, Siti Nurfadhlina Mohd-Noor, Nadja Nolte, Boon Chin Tan
Summary: RNA-guided genomic transcriptional regulation tools, CRISPRi and CRISPRa, derived from the CRISPR/Cas9 system, utilize a catalytically dead Cas9 to achieve transcriptional interference. Despite its potential benefits, challenges and limitations such as off-target effects, PAM sequence requirements, and labeling as genetically modified organisms are faced by the CRISPR/dCas9 system.
Review
Oncology
Alejandro Martinez-Escobar, Benjamin Luna-Callejas, Eva Ramon-Gallegos
Summary: Cancer treatment is challenging due to high resistance, but recent advancements in therapies have improved effectiveness and reduced toxic and invasive effects. Drug development for cancer is costly and time-consuming, but repurposed drugs combined with CRISPR-dCas9-based artificial transcription factors show potential for improving efficacy and reducing mortality.
FRONTIERS IN ONCOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Hongtong Lu, Qingfei Zhang, Shasha He, Sha Liu, Zhigang Xie, Xiaoyuan Li, Yubin Huang
Summary: A PtUTP-F nanoplatform was developed for targeted CRISPR/dCas9 activation of CT45, achieving synergistic and individualized treatment. PtUTP-F can efficiently transfect different cells and enhance sensitivity to Pt(II) drugs, serving as a novel strategy for treatment.
Article
Chemistry, Analytical
Tingting Liang, Xiaojie Qin, Yuyuan Zhang, Yu Yang, Yu Chen, Lin Yuan, Feng Liu, Zhizhong Chen, Xinchun Li, Fan Yang
Summary: This study presents an electrochemical CRISPR/dCas9 sensor for sensitive and specific detection of ctDNA at a single-nucleotide resolution. The sensor exhibits superior sensitivity and a broad dynamic range, and can accurately analyze cellular genomic DNA in blood samples from cancer patients.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Emily A. Saunderson, Hector Huerga Encabo, Julie Devis, Kevin Rouault-Pierre, Marion Piganeau, Christopher G. Bell, John G. Gribben, Dominique Bonnet, Gabriella Ficz
Summary: Aging is associated with abnormal DNA methylation, which can affect hematopoiesis. This study demonstrates that the methylation of the p15 locus has a functional relevance on hematopoiesis, and the epigenetic changes can be maintained in human stem/progenitor cells. These findings suggest that other aging-associated aberrant DNA methylation may also impact hematopoiesis in vivo.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Genetics & Heredity
Brittany Chapman, Jeong Hoon Han, Hong Jo Lee, Isabella Ruud, Tae Hyun Kim
Summary: The engineering of CRISPR and Cas9 system has allowed for diverse applications beyond DNA cleavage. By combining nuclease-deactivated Cas9 with transcriptional effector domains, gene activation (CRISPRa) or repression (CRISPRi) can be achieved. In this study, the effectiveness of CRISPR-mediated transcriptional regulation was demonstrated in chicken DF-1 cells using various CRISPRa and CRISPRi systems, resulting in significant gene upregulation and downregulation. It was also found that the location of guide RNAs plays a crucial role in targeted gene regulation. RNA sequencing analysis confirmed the specificity and minimal off-target effects of CRISPRa and CRISPRi-based transcriptional modulation in chicken cells.
Article
Biology
Byung-Sun Park, Heeju Jeon, Sung-Gil Chi, Tackhoon Kim
Summary: CRISPR-based screens are powerful tools for drug discovery, but low-activity guide RNAs can lead to variable changes in phenotype and false negative results. In this study, an approach was developed to correct the bias in CRISPR screening data by using the actual frequency of indel mutations generated by guide RNAs. The method was shown to improve the efficiency of identifying essential genes.
Article
Biotechnology & Applied Microbiology
Xinyue Song, Tao Yan, Feng Tian, Fengyan Li, Linlin Ren, Qiong Li, Shusheng Zhang
Summary: The research team developed a highly localized drug nanocarrier for in situ release of DOX molecules at their action site to directly interfere with DNA activity. This new nanotheranostic agent demonstrated good targetability, significant inhibition on tumor growth, and high cell apoptosis ratio at a lower concentration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Zhibo Wang, Zihang He, Zhujun Liu, Ming Qu, Caiqiu Gao, Chao Wang, Yucheng Wang
Summary: DNA-protein interaction is crucial in biological processes, but current technologies based on DNA hybridization lack specificity and efficiency. To overcome this, we developed a DNA-binding protein capture technology, R-ChIP-dCas9, using the CRISPR-dCas9 system and transient genetic transformation. This method enables the identification of upstream transcription factors, such as BpNAC090 and its potential regulators.
Letter
Biology
Jianlin Zhang, Shimin Li, Ni Xie, Guohui Nie, Aifa Tang, Xian-En Zhang, Minmin Liang, Xiyun Yan
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Virology
Wei Li, Ji Liu, Yuanyuan Liu, Qin Li, Wen Yin, Kevin K. Wanderi, Xiaowei Zhang, Zhiping Zhang, Xian-En Zhang, Zongqiang Cui
Summary: This study reveals the dynamic podosome-mediated entry of HIV-1 into macrophages, where the core and ring structures of podosomes play complex roles in viral entry, with the CCR5 coreceptor recruited to form specific clusters. The podosome facilitates HIV-1 entry with a rotation mode triggered by dynamic actin.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Yang Zheng, Qing Zhang, Ashaq Ali, Ke Li, Nan Shao, Xiaoli Zhou, Zhiqin Ye, Xiaomin Chen, Shanshan Cao, Jing Cui, Juan Zhou, Dianbing Wang, Baidong Hou, Min Li, Mengmeng Cui, Lihua Deng, Xinyi Sun, Qian Zhang, Qinfang Yang, Yong li, Hui Wang, Yake Lei, Bo Yu, Yegang Cheng, Xiaolin Tong, Dong Men, Xian-En Zhang
Summary: The majority of convalescent COVID-19 patients displayed sustained high levels of antibodies for over six months, while a small percentage showed declining levels to undetectable, warranting further attention. This indicates a typical dynamic of acquired immunity in the humoral immune response against SARS-CoV-2 infection in COVID-19 patients.
Article
Nanoscience & Nanotechnology
Yingxin Ma, Guobin Mao, Guoqiang Wu, Minghai Chen, Fujun Qin, Luping Zheng, Xian-En Zhang
Summary: The study created a dual color pseudo-SARS-CoV-2 virus using a pseudovirus strategy, allowing tracking of virus movement and fate of labeled components. Pseudo-SARS-CoV-2 mainly entered host cells through endocytosis, with infection efficiency correlated to the expression of ACE2 receptor on host cell surface. The dual-color fluorescently labeled pseudovirus system is believed to be a useful tool for various purposes in SARS-CoV-2/COVID-19 research.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biology
Rui Gong, Dianbing Wang, Ghulam Abbas, Shimin Li, Qian Liu, Mengmeng Cui, Xian-En Zhang
Summary: This study introduces a genetically encoded switch-on mNeonGreen2-based biosensor for detecting caspase-3 activity. The biosensor demonstrated shortened response time, higher sensitivity, and prolonged functional shelf life in detecting caspase-3 compared to existing counterparts. Additionally, the biosensor was used to evaluate the effect of various drugs on inducing apoptosis in HeLa and MCF-7 tumor cells and inhibiting Zika virus invasion.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Microbiology
Hongmei Shi, Ting Li, Jintian Xu, Jifang Yu, Shanshan Yang, Xian-En Zhang, Shengce Tao, Jing Gu, Jiao-Yu Deng
Summary: Through experiments on Escherichia coli, this study found that MgrB affects susceptibility to trimethoprim by modulating the expression of folA with the involvement of PhoP/Q. Furthermore, the results suggest that PhoP may not directly regulate the transcription of folA.
FRONTIERS IN MICROBIOLOGY
(2021)
Letter
Virology
Xiaohua Chen, Juan Zhou, Chen Chen, Baidong Hou, Ashaq Ali, Feng Li, Zhaolin Hua, Yingtao Wu, Qin Yang, Min Chen, Rong Zhang, Qianchuan Huang, Jinya Ding, Xian-En Zhang, Dong Men
Review
Chemistry, Multidisciplinary
Dian-Bing Wang, Meng-Meng Cui, Min Li, Xian-En Zhang
Summary: Detecting Bacillus anthracis quickly and sensitively poses challenges due to the need for accurate differentiation from other Bacillus species, preserving spores for direct detection, and rapid testing in complex samples without prior processing. Various biosensors offer potential solutions with different detection targets, molecular recognition elements, and sensing systems, utilizing nanotechnology to enhance performance. Further advancements are needed to address remaining challenges and improve practicality in B. anthracis detection methods.
ACCOUNTS OF CHEMICAL RESEARCH
(2021)
Article
Electrochemistry
Jin Song, Yiwei Li, Dingkun Ke, Dianbing Wang, Xian-En Zhang
Summary: By integrating graphene with carbon microelectrode arrays, a sensitive biosensor platform has been developed for studying biofilm formation and screening antibiofilm drugs. The graphene-modified microelectrode arrays demonstrated better performance and higher specificity in detecting biofilm growth.
ELECTROCHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Minghai Chen, Chuang Yan, Luping Zheng, Xian-En Zhang
Summary: The study developed near-infrared BiFC and TriFC systems using miRFP670nano, enabling efficient visualization of protein interactions. The sensor system showed faster maturation rate, higher photostability, and cellular stability compared to existing systems, demonstrating its potential for various applications in cell imaging studies.
Article
Chemistry, Physical
Cuihua Wei, Juan Zhou, Tiangang Liu, Wanqi Zhao, Xian-En Zhang, Dong Men
Summary: The study developed a self-assembled enzymatic nanowire with a unique interface, improving enzyme stability and catalytic activity in organic solvents. By fusing JTA with Sup35 to construct JTAnw, the reaction time in organic solvents was significantly reduced, and the thermal and pH stability of the enzyme was enhanced.
Article
Chemistry, Multidisciplinary
Weiwei Zhu, Ti Fang, Wenjing Zhang, Ao Liang, Hui Zhang, Zhi-Ping Zhang, Xian-En Zhang, Feng Li
Summary: Dps, a natural protein nanocage, has been developed for antioxidant treatment by inhibiting the generation of ROS. With its ferroxidase activity, Dps not only protects cells from oxidative stress but also alleviates inflammation induced by ROS. This unique material shows great potential in various applications such as antiaging, anti-inflammation, and cosmetics.
Article
Biology
Song Lu, Dianbing Wang, Yu Hou, Dongge Guo, Yulin Deng, Xian-En Zhang
Summary: This study successfully reduced the number of fluorescent modules entering the nucleus by redesigning the CRISPR/dCas9-SunTag labeling system, resulting in decreased background fluorescence and increased signal-to-background ratio.
SCIENCE CHINA-LIFE SCIENCES
(2021)
