期刊
BIOSENSORS-BASEL
卷 11, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/bios11010016
关键词
glucose; self-powered; biosensor; biofuel cell; NAD-glucose dehydrogenase; glucose oxidase; horseradish peroxidase; reduced graphene oxide
资金
- Suranaree University of Technology [BRO1-102-62-12-01]
This research presents a self-powered glucose sensor based on a biofuel cell, which efficiently detects glucose with high sensitivity using immobilized redox enzymes. The combination of carbon nanostructures and multi-enzyme cascade allows the sensor to operate under air-saturating conditions.
Biofuel cells allow for constructing sensors that leverage the specificity of enzymes without the need for an external power source. In this work, we design a self-powered glucose sensor based on a biofuel cell. The redox enzymes glucose dehydrogenase (NAD-GDH), glucose oxidase (GOx), and horseradish peroxidase (HRP) were immobilized as biocatalysts on the electrodes, which were previously engineered using carbon nanostructures, including multi-wall carbon nanotubes (MWCNTs) and reduced graphene oxide (rGO). Additional polymers were also introduced to improve biocatalyst immobilization. The reported design offers three main advantages: (i) by using glucose as the substrate for the both anode and cathode, a more compact and robust design is enabled, (ii) the system operates under air-saturating conditions, with no need for gas purge, and (iii) the combination of carbon nanostructures and a multi-enzyme cascade maximizes the sensitivity of the biosensor. Our design allows the reliable detection of glucose in the range of 0.1-7.0 mM, which is perfectly suited for common biofluids and industrial food samples.
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