期刊
BMC VETERINARY RESEARCH
卷 11, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12917-015-0423-8
关键词
Echinococcus granulosus; Adult worm; Excretory/secretory products; Antigenic protein; 2-dimensional gel electrophoresis; MALDI-TOF/TOF
资金
- National Natural Science Foundation [81371841, 81371842]
- National S & T Major Program [2012ZX10004-201]
Background: Cystic echinococcosis, which is caused by Echinococcus granulosus, is one of the most widespread zoonotic helminth diseases that affects humans and livestock. Dogs, which harbor adult worms in their small intestines, are a pivotal source of E. granulosus infection in humans and domestic animals. Therefore, novel molecular approaches for the prevention and diagnosis of this parasite infection in dogs need to be developed. Results: In this study, we performed proteomic analysis to identify excretory/secretory products (ES) and antigenic proteins of E. granulosus adult worms using two-dimensional electrophoresis, tandem matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/TOF), and Western blotting of sera from infected dogs. This study identified 33 ES product spots corresponding to 9 different proteins and 21 antigenic protein spots corresponding to 13 different proteins. Six antigenic proteins were identified for the first time. Conclusions: The present study extended the existing proteomic data of E. granulosus and provides further information regarding host-parasite interactions and survival mechanisms. The results of this study contribute to vaccination and immunodiagnoses for E. granulosus infections.
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