4.7 Article

Influence of critical factors on nitrogen removal contribution by anammox and denitrification in an anammox-inoculated wastewater treatment system

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ELSEVIER
DOI: 10.1016/j.jwpe.2020.101868

关键词

Denitrification; Anammox; Nitrogen remove; Environmental factors; Wastewater treatment system

资金

  1. Natural Science Foundation of Guangdong Province [2019B1515120066]
  2. National Natural Science Foundation of China [31870100, 91851111]

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This study investigated the effects of environmental factors on denitrification and anammox activity in a wastewater treatment system, with results showing that temperature increase promoted both activities. Denitrification was more sensitive to pH values, while anammox showed higher adaptability. Anammox also exhibited stronger tolerance to higher dissolved oxygen concentration compared to denitrification. Organic matter significantly promoted denitrification activity, while anammox activity was inhibited by glucose but enhanced by acetate, sodium formate, and pyruvate. The findings provide useful information for improving nitrogen removal efficiency by regulating environmental factors to facilitate collaboration between denitrification and anammox.
Suitable environmental conditions facilitate the nitrogen removal in the wastewater treatment system. However, it remains unknown about how critical environmental factors regulating the activity of denitrification and anammox in the anammox-inoculated wastewater treatment system. In this study, the effect of temperature, pH, dissolved oxygen, organic matter, and substrates on denitrification and anammox activity was investigated with the nitrogen isotope pairing method. Results showed that the increase of temperature promoted the activities of both denitrification and anammox, and the activity of anammox was higher than that of denitrification at 25 degrees C-30 degrees C. Denitrification was more sensitive to acidic and alkaline pH values with the highest activity observed at neutral pH, but anammox has a more robust adaptability and the reaction rate remained relatively higher over a wider pH range. Moreover, anammox showed a stronger tolerance than denitrification to higher dissolved oxygen (DO) concentration. In addition, organic matter promoted the activity of denitrification significantly, while the anammox activity was inhibited by glucose, but enhanced by acetate, sodium formate and pyruvate. The denitrification and anammox reaction rates responding to nitrate were fitted well to Michaelis-Menten kinetics, but showing a linear relationship with nitrite. The results presented here offered useful information on how to regulate the environmental factors to improve the nitrogen removal efficiency from wastewater by facilitating the collaboration between denitrification and anammox.

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