期刊
ANNALS OF TRANSLATIONAL MEDICINE
卷 9, 期 1, 页码 -出版社
AME PUBL CO
DOI: 10.21037/atm-20-2506
关键词
Immunoglobulin A nephropathy (IgAN); microbiome; metabolome; metabolic network
资金
- National Natural Science Foundation of China [81671596]
- National Science Foundation for Young Scientists of China [31700795]
- Education Innovation Project for Graduates of Guangzhou, China [2020XLLT10]
- Twenty-one Teaching Reform Project of Jinan University [JG2019044]
This study analyzed the gut microbiota and metabolic patterns of IgAN patients and healthy controls, finding that changes in the gut microbiota affected the metabolism and absorbance of key metabolites. The study identified six pivotal metabolites connecting the metabolic networks of the gut and blood, as well as associations between specific metabolites and the classification of glomerular sclerosis. The results establish a relational network between microbiota, fecal metabolites, and serum metabolites in IgAN, offering potential value for therapeutic applications.
Background: Immunoglobulin A nephropathy (IgAN) is the most common form of primary glomerulonephritis. The intestinal microbial ecosystem and metabolic network of IgAN have not been systematically analyzed. The present study aims to improve understanding of the gut microbiota and its metabolic capabilities to facilitate the development of diagnostic, therapeutic, and prognostic methods for IgAN. Methods: We characterized the gut microbiota and metabolic patterns of fecal and serum samples of IgAN patients and healthy controls from the south of China using 16s ribosomal RNA gene sequencing and liquid chromatography-tandem mass spectrometry, respectively, and bioinformatics approaches. Results: We found that the relative abundances of Streptococcus and Enterococcus were higher in IgAN patients, whereas Bacteroidetes and Bacteroides were lower. Changes in the gut microbiota of IgAN affected the metabolism and absorbance of microbiota-associated metabolites, in particular polyunsaturated fatty acids, free amino acid, and oligopeptides, and activated the phenylalanine metabolism pathway, thereby constructing a unique metabolic system of IgAN. We identified six pivotal metabolites, including bilirubin, trimethoprim, stearamide, phenylalanine, cis-9,10-epoxystearic acid, and phosphatidylethanolamine 17:0, that connected the metabolic networks of the gut and blood. Additionally, 5-hydroxyeicosatetraenoic acid and 5-hydroxy-6E,8Z,11Z-eicosatrienoic acid were shown to be associated with the classification of glomerular sclerosis. Conclusions: We establish a relational network between microbiota, fecal metabolites, and serum metabolites in IgAN. The core microbiota and metabolites identified have promising value in therapeutic applications.
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