期刊
BIOMOLECULES
卷 10, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/biom10121593
关键词
phospholipase A(2); preadipocytes; prostaglandins; adipokines; cytokines; EP receptors
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/23236-4, 2015/24701-3, 2017/197339]
Adipose tissue secretes proinflammatory mediators which promote systemic and adipose tissue inflammation seen in obesity. Group IIA (GIIA)-secreted phospholipase A(2) (sPLA(2)) enzymes are found to be elevated in plasma and adipose tissue from obese patients and are active during inflammation, generating proinflammatory mediators, including prostaglandin E-2 (PGE(2)). PGE(2) exerts anti-lipolytic actions and increases triacylglycerol levels in adipose tissue. However, the inflammatory actions of GIIA sPLA(2)s in adipose tissue cells and mechanisms leading to increased PGE(2) levels in these cells are unclear. This study investigates the ability of a representative GIIA sPLA(2), MT-III, to activate proinflammatory responses in preadipocytes, focusing on the biosynthesis of prostaglandins, adipocytokines and mechanisms involved in these effects. Our results showed that MT-III induced biosynthesis of PGE(2), PGI(2), MCP-1, IL-6 and gene expression of leptin and adiponectin in preadipocytes. The MT-III-induced PGE(2) biosynthesis was dependent on cytosolic PLA(2) (cPLA(2))-alpha, cyclooxygenases (COX)-1 and COX-2 pathways and regulated by a positive loop via the EP4 receptor. Moreover, MT-III upregulated COX-2 and microsomal prostaglandin synthase (mPGES)-1 protein expression. MCP-1 biosynthesis induced by MT-III was dependent on the EP4 receptor, while IL-6 biosynthesis was dependent on EP3 receptor engagement by PGE(2). These data highlight preadipocytes as targets for GIIA sPLA(2)s and provide insight into the roles played by this group of sPLA(2)s in obesity.
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