Efficient bioelectrocatalytic CO2 reduction on gas-diffusion-type biocathode with tungsten-containing formate dehydrogenase

A new gas-diffusion-type biocathode was constructed for carbon dioxide (CO2) reduction. In this work, tungsten-containing formate dehydrogenase (FoDH1), which is a promising enzyme for interconversion of formate and CO2, was used as a catalyst and was absorbed on a Ketjen Black (KB)-modified electrode. We used 1,1 '-trimethylene- 2,2 '-bipyridinium dibromide as a mediator, and the hydrophobicity of the FoDH1-absorbed electrode was optimized according to the weight ratio of the polytetrafluoroethylene binder to KB. We achieved cathodic current densities of about 20 mA cm(-2) under mild and quiescent conditions (neutral pH, atmospheric pressure, and room temperature). (C) 2016 Elsevier B.V. All rights reserved.