Physiological Basis of Combined Stress Tolerance to Low Phosphorus and Drought in a Diverse Set of Mungbean Germplasm

To understand the physiological basis of tolerance to combined stresses to low phosphorus (P) and drought in mungbean (Vigna radiata (L.) R. Wilczek), a diverse set of 100 accessions were evaluated in hydroponics at sufficient (250 mu M) and low (3 mu M) P and exposed to drought (dehydration) stress. The principal component analysis and ranking of accessions based on relative values revealed that IC280489, EC397142, IC76415, IC333090, IC507340, and IC121316 performed superior while IC119005, IC73401, IC488526, and IC325853 performed poorly in all treatments. Selected accessions were evaluated in soil under control (sufficient P, irrigated), low P (without P, irrigated), drought (sufficient P, withholding irrigation), and combined stress (low P, withholding irrigation). Under combined stress, a significant reduction in gas exchange traits (photosynthesis, stomatal conductance, transpiration, instantaneous water use efficiency) and P uptake in seed and shoot was observed under combined stress as compared to individual stresses. Among accessions, IC488526 was most sensitive while IC333090 and IC507340 exhibited tolerance to individual or combined stress. The water balance and low P adaptation traits like membrane stability index, relative water content, specific leaf weight, organic acid exudation, biomass, grain yield, and P uptake can be used as physiological markers to evaluate for agronomic performance. Accessions with considerable tolerance to low P and drought stress can be either used as 'donors' in Vigna breeding program or cultivated in areas with limited P and water availability or both.

Automated summary

The study found that under combined stresses of low phosphorus and drought, gas exchange traits and phosphorus uptake in mungbean were significantly reduced, with IC488526 being the most sensitive and IC333090 and IC507340 exhibiting tolerance to individual or combined stresses. Water balance and low P adaptation traits can be used as physiological markers for evaluating agronomic performance, and accessions with considerable tolerance to low P and drought stress can be utilized as 'donors' in Vigna breeding programs.