Article
Biotechnology & Applied Microbiology
Zicheng Huang, Chen Zhong, Jingxuan Dai, Shuning Li, Mingmin Zheng, Yongjin He, Mingzi Wang, Bilian Chen
Summary: A novel two-stage cultivation method was developed for Porphyridium cruentum microalgae using different wavelengths of light-emitting diodes to optimize growth and bioactive substances production. Green wavelength enhanced cell growth and bioactive compounds synthesis, while white wavelength promoted fatty acid accumulation. Compared to one-stage cultivation, the two-stage cultivation showed higher efficiency in microalgal growth and productivity.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Food Science & Technology
Tao Li, Jin Xu, Weinan Wang, Zishuo Chen, Chulin Li, Hualian Wu, Houbo Wu, Wenzhou Xiang
Summary: A novel fractional extraction strategy was developed to efficiently extract high-value compounds from Porphyridium, including phycoerythrin, lipids, and polysaccharides. Although crude phycoerythrin showed the best antioxidant activity, further purification is required for downstream applications.
Article
Biotechnology & Applied Microbiology
Liang Ji, Shaohua Li, Cheng Chen, Haojie Jin, Haizhen Wu, Jianhua Fan
Summary: Porphyridium purpureum is a mesophilic, unicellular red alga rich in phycoerythrin, sulfate polysaccharides, and polyunsaturated fatty acids. Nitrogen deficiency inhibits its growth, resulting in cell yellowing and thickening of the extracellular viscous sheath. This study shows that under nitrogen stress, P. purpureum increases the content of total lipids and exopolysaccharides.
BIORESOURCES AND BIOPROCESSING
(2021)
Article
Food Science & Technology
Chulin Li, Houbo Wu, Wenzhou Xiang, Hualian Wu, Na Wang, Jiayi Wu, Tao Li
Summary: The study evaluated the production and fluorescence characteristics of phycoerythrin of three strains of Porphyridium, with P. purpureum SCS-02 showing the highest biomass, phycoerythrin content, and yield. The phylogenetic tree and fluorescence characteristics suggested that P. purpureum and P. cruentum might be the same Porphyridium species.
Article
Agricultural Engineering
Min Seo Jeon, Sang-Il Han, Minsol Jeon, Yoon-E Choi
Summary: Genetic manipulation of Porphyridium sp. increased the production of phycoerythrin via a CRISPR/Cas9 RNP delivery system targeting the Chs1 gene. Mutants showed enhanced phycoerythrin contents without affecting growth or polysaccharide production, indicating a potential for commercial production if Porphyridium is chosen as the source.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Davide Liberti, Paola Imbimbo, Enrica Giustino, Luigi D'Elia, Melanie Silva, Luisa Barreira, Daria Maria Monti
Summary: The antioxidant and anti-inflammatory activities of sulfated exopolysaccharides (s-EPSs) and phycoerythrin (PE) from the red marine microalga Porphyridium cruentum (CCALA415) were evaluated. s-EPSs showed biocompatibility and high antioxidant activity, while PE demonstrated powerful antioxidant activity. Both molecules exhibited anti-inflammatory activity and facilitated tissue regeneration. Therefore, microalgae-derived compounds are excellent sources of antioxidants for various applications.
Article
Environmental Sciences
Hao-Chan Yin, Ji-Kang Sui, Tian-Li Han, Tian-Zhong Liu, Hui Wang
Summary: This study aimed to establish the integration bioprocess of B-PE and EPS production from red microalga Porphyridium cruentum. The effects of different growth medium and CO2 concentration on biomass and B-PE and EPS contents were evaluated. Optimal conditions for extracting B-PE and EPS were determined, and high-purity B-PE with significant physicochemical properties of EPS were obtained.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Davide Liberti, Paola Imbimbo, Enrica Giustino, Luigi D'Elia, Giarita Ferraro, Angela Casillo, Anna Illiano, Gabriella Pinto, Maria Chiara Di Meo, Gerardo Alvarez-Rivera, Maria Michela Corsaro, Angela Amoresano, Armando Zarrelli, Elena Ibanez, Antonello Merlino, Daria Maria Monti
Summary: A biorefinery approach was developed to extract high-value bioproducts, including exopolysaccharides, phycoerythrin, and β-carotene, from the culture of Porphyridium cruentum.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Ai Hua Zhang, Bo Feng, Han Zhang, Jinshun Jiang, Daofeng Zhang, Yi Du, Zheng Cheng, Jianke Huang
Summary: In this study, different percentages of diluted swine wastewater were used to cultivate the red alga Porphyridium purpureum, and its microalgal growth, nutrient removal, polysaccharide production, and phycoerythrin production were evaluated. The results showed that 60% diluted swine wastewater resulted in the best growth and nutrient removal, and high concentrations of polysaccharide and phycoerythrin were produced. This indicates that P. purpureum has great potential in treating wastewater and producing high-value byproducts.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Entomology
Somaya Naser El Deen, Klaas van Rozen, Hellen Elissen, Piet van Wikselaar, Istvan Fodor, Rommie van der Weide, Elise Federica Hoek-van den Hil, Arya Rezaei Far, Teun Veldkamp
Summary: This study assessed the growth and waste conversion of black soldier fly larvae (BSFL) reared on different waste streams. The results showed that fast food waste was the most suitable substrate for BSFL, leading to the highest larval growth and waste conversion efficiency. Slaughter waste, pig manure, and mushroom stems also showed good potential for conversion. BSFL are considered a commercially viable solution for global organic waste problems.
Article
Chemistry, Applied
Zicheng Huang, Shuai Guo, Zheng Guo, Yongjin He, Bilian Chen
Summary: A novel and environmentally friendly one-step extraction process for extracting phycobiliproteins and PUFAs from wet Porphyridium biomass has been developed and optimized using the TPP process. The results showed that the TPP process provided the best extraction performance and cost-effectiveness for extracting both phycobiliproteins and PUFAs.
Article
Biotechnology & Applied Microbiology
Liang Ji, Yueling Liu, Jianquan Luo, Jianhua Fan
Summary: Microalgae are a promising alternative source for natural phycobiliprotein production, but their high production cost limits their viability. This study developed a new extraction and purification method to recover B-phycoerythrin (B-PE) from Porphyridium purpureum using a freeze-thaw-assisted aqueous two-phase system (FT-ATPS). The results showed that low light induced B-PE synthesis but hindered growth. Stepwise light supplementation and the green freeze-thaw method improved production and extraction efficiency. The purity and concentration of B-PE in the crude extract also had a significant effect on the purification process. Overall, this method can significantly reduce costs and have more environmental benefits.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Microbiology
Andrei B. Borovkov, Irina N. Gudvilovich, Irina A. Maltseva, Olga A. Rylkova, Yevhen Maltsev
Summary: This study aims to investigate the effects of different ways of supplying CO2 on P. purpureum biomass and reveals that atomization of carbon dioxide can significantly enhance productivity and protein synthesis rate in P. purpureum. Moreover, significant changes in cell morphology were observed.
Article
Agricultural Engineering
Ajeet Singh Chauhan, Anil Kumar Patel, Chiu-Wen Chen, Jo-Shu Chang, Philippe Michaud, Cheng-Di Dong, Reeta Rani Singhania
Summary: Considering the increasing health concerns, urban population tends to adopt a healthy lifestyle and incorporate nutritional food supplements to mitigate common health risks. The consumption of omega-3 and omega-6 PUFAs is on the rise, thus alternative commercial production methods are being developed. Microbial sources are emerging platforms to meet the global demand for omega PUFAs. Marine oleaginous protist Aurantiochytrium sp. has been identified as a potential source for substantial production of DHA and SFA. The objective of this research was to enhance PUFA yield by optimizing glucose concentration and nitrogen ratio. The maximum lipid and DHA yield and content were achieved at 30 g/L glucose, while the relative PUFA content was highest at 10 g/L glucose, with remaining SFA potentially usable for biodiesel.
BIORESOURCE TECHNOLOGY
(2023)
Article
Environmental Sciences
Jim Junhui Huang, Peter Chi Keung Cheung
Summary: The study showed that Porphyridium cruentum exhibited increased production of fatty acids and carotenoids under cold stress, leading to enhanced production of biodiesel feedstock. Principal component analysis indicated that the biosynthetic pathways of fatty acids and carotenoids in this microalga were influenced by cold stress.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biotechnology & Applied Microbiology
Niels-Henrik Norsker, Maria Cuaresma, Jeroen de Vree, Mari Carmen Ruiz-Dominguez, Maria Carmen Marquez Garcia, Pauliina Uronen, Maria J. Barbosa, Rene Wijffels
Summary: In this study, oil production using Neochloris oleoabundans was tested in a horizontal soft sleeve tubular reactor in southern Spain. The biomass productivity was higher during the nitrogen replete phase, while the lipid productivity remained stable during the nitrogen depletion phase. Additionally, nitrogen depletion was used to enhance fatty acid formation, with the inverse nitrogen quota in the biomass predicting the fatty acid content.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
M. C. Ruiz-Dominguez, J. L. Fuentes, J. A. Mendiola, P. Cerezal-Mezquita, J. Morales, C. Vilchez, E. Ibanez
Summary: This study investigated a new cyanobacterium Geitlerinema sp. from the Chilean coastal desert as a rich source of bioactive compounds. The study used response surface methodology to optimize extraction conditions and identified various bioactive compounds with potential nutritional, pharmaceutical, and industrial applications. The results suggest that Geitlerinema sp. has promising functional properties for sustainable and cost-effective bioactive compounds.
FOOD AND BIOPRODUCTS PROCESSING
(2021)
Article
Chemistry, Medicinal
Mari Carmen Ruiz-Dominguez, Francisca Salinas, Elena Medina, Barbara Rincon, Mari angeles Martin, Mari Carmen Gutierrez, Pedro Cerezal-Mezquita
Summary: In this study, fucoxanthin was successfully extracted from Phaeodactylum tricornutum using supercritical fluid extraction (SFE) and the extracted diatom-like substrate was utilized for bioenergy production through anaerobic digestion (AD) processes. The optimization of various factors resulted in high yield, purity, and recovery of fucoxanthin. The extracted diatom and the whole diatom both showed potential for biogas production, with different methane yields. Therefore, Phaeodactylum tricornutum can serve as a valuable source of both fucoxanthin and methane, contributing to the sustainability of algae-biorefinery processes.
Article
Green & Sustainable Science & Technology
Pedro Cerezal-Mezquita, Waldo Bugueno-Munoz
Summary: Minimizing agricultural losses and utilizing solar energy technologies are the challenges faced by the Antofagasta region in Chile. The study focused on the effect of drying on the quality of carrot strips using an indirect solar dryer. The results showed a detectable color change and identified the Weibull distribution model as the best fit. The drying installation was also found to maintain temperature values during periods without solar radiation.
Article
Agriculture, Dairy & Animal Science
Pedro Cerezal-Mezquita, Carolina - Espinosa-Alvarez, Marjorie J. Jauregui-Tirado, Carolina Jaime-Matus, Jenifer Palma-Ramirez, Mari Carmen Ruiz-Dominguez
Summary: This study characterized a novel non-defatted meal feed product in terms of its physical and chemical properties. The product exhibited different rheological behaviors at different temperatures and contained significant amounts of proteins, fatty acids, and carotenoids.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Mari Carmen Ruiz-Dominguez, Barbara Rincon, Maria de los Angeles Martin, Maria del Carmen Gutierrez, Francisca Salinas, Elena Medina, Pedro Cerezal
Summary: The study evaluated the extraction of fucoxanthin and polyunsaturated fatty acids from Isochrysis galbana using supercritical fluid extraction (SFE) and valorized the spent microalga through anaerobic digestion (AD) to produce biogas. The results showed that co-digestion with other substrates increased methane production.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mari Carmen Ruiz-Dominguez, Elena Medina, Francisca Salinas, Waldo Bugueno, Juan-Luis Fuentes, Carlos Vilchez, Ines Garbayo, Pedro Cerezal-Mezquita
Summary: Microalgae have great potential for biotechnology due to their ability to grow in diverse environments and contain useful bioactive compounds. This study evaluated the effects of supercritical fluid extraction (SFE) on the recovery of bioactive and antioxidant compounds from Coccomyxa onubensis microalgae. The study found that temperature, pressure, and co-solvent concentration were significant factors affecting the extraction process.
Article
Biotechnology & Applied Microbiology
Mari Carmen Ruiz-Dominguez, Jose Antonio Mendiola, Jose David Sanchez-Martinez, Monica Bueno, Pedro Cerezal-Mezquita, Elena Ibanez
Summary: This study reveals the potential of the brown seaweed Durvillaea antarctica, commonly consumed in Chile, as an important food with antioxidant and neuroprotective properties. The bioactivity of the seaweed extracts was evaluated using conventional and pressurized liquid extraction methods. The results showed that pressurized liquid extraction improved the extraction yield and antioxidant activities compared to conventional extraction. Furthermore, the study identified the optimal conditions for obtaining extracts with high yields and neuroprotective effects.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Chemistry, Physical
Renata Vardanega, Francisca Salinas Fuentes, Jenifer Palma, Waldo Bugueno-Munoz, Pedro Cerezal-Mezquita, Mari Carmen Ruiz-Dominguez
Summary: The development of food waste biorefineries based on clean technologies aims to reduce food waste and its impact on climate change. This study developed a sequential extraction process using supercritical fluid extraction (SFE) and pressurized liquid extraction (PLE) to recover valuable compounds from granadilla seeds. The optimized SFE conditions yielded a PUFA-rich oil, while PLE at 70°C and 10 MPa with 75% EtOH was more efficient in extracting phenolic compounds from the defatted seeds compared to ultrasound-assisted extraction (UAE).
JOURNAL OF SUPERCRITICAL FLUIDS
(2023)
Article
Chemistry, Medicinal
Mari Carmen Ruiz-Dominguez, Maria Robles, Lidia Martin, Alvaro Beltran, Riccardo Gava, Maria Cuaresma, Francisco Navarro, Carlos Vilchez
Summary: This study evaluated the recovery of valuable molecules with anti-inflammatory and antimicrobial activity from the acidophilic microalga Coccomyxa onubensis using green technologies based on ultrasound-assisted extraction (UAE). Significant differences in extraction recovery were observed between ultrasonic bath, ultrasonic probe, and conventional extraction. UAE-based extracts exhibited higher antimicrobial activity compared to solvent-based extracts.
Article
Chemistry, Multidisciplinary
Renata Vardanega, Francisca Salinas Fuentes, Jenifer Palma, Waldo Bugueno-Munoz, Pedro Cerezal-Mezquita, Mari Carmen Ruiz-Dominguez
Summary: Valuable products, including carotenoids, phenolic compounds, and pectin, were obtained from granadilla peel using green technologies. Pressurized liquid extraction (PLE) and CO2-expanded ethanol (GXE) were effective methods for extracting carotenoids and phenolic compounds, while pressurized hot water extraction (PHWE) was suitable for extracting pectin. Further studies are needed to characterize the obtained pectin, but this research establishes a foundation for a biorefinery to utilize granadilla wastes.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2023)
Article
Chemistry, Multidisciplinary
Mari Carmen Ruiz-Dominguez, Constanza Toledo, Daniel Ordenes, Carlos Vilchez, Paula Ardiles, Jenifer Palma, Pedro Cerezal
Summary: The study compared the effects of different direct transesterification methods on the omega-3/6 composition of extracts from Phaeodactylum tricornutum, finding that alkali-catalyst method improves the extraction efficiency of PUFA. Additionally, the relative content of fatty acids is influenced by pre-extracted lipids, and direct transesterification methods help to more accurately assess the application value of biomass in the food industry.
ACTA CHIMICA SLOVENICA
(2021)