Review
Biochemistry & Molecular Biology
Juan Garcia-Arriaza, Mariano Esteban, Daniel Lopez
Summary: There is a need for a highly effective vaccine against the emerging chikungunya virus, and this review discusses the development of potential vaccine candidates. It highlights the importance of understanding the immune responses induced by these candidates in animal models before progressing to human clinical trials.
Letter
Medicine, General & Internal
Lucy Deng, Laura K. Lopez, Catherine Glover, Patrick Cashman, Renee Reynolds, Kristine Macartney, Nicholas Wood
Summary: This study utilizes data from Australia's vaccine safety surveillance system to investigate the adverse effects of the modified vaccinia Ankara-Bavarian Nordic vaccine.
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
(2023)
Article
Immunology
Michal Krolik, Lucia Csepregi, Fabienne Hartmann, Celine Engetschwiler, Lukas Flatz
Summary: The study investigated the safety and efficacy of reverse genetically engineered recombinant lymphocytic choriomeningitis virus (rLCMV) in mice lacking a functional type I interferon system. The results showed that rLCMV provided excellent protection in immunocompromised mice by inducing multifunctional cytotoxic T cells.
Article
Hematology
Suzy A. Eldershaw, Hayden Pearce, Charlotte F. Inman, Karen P. Piper, Ben Abbotts, Christine Stephens, Samantha Nicol, Wayne Croft, Richard Powell, Jusnara Begum, Graham Taylor, Jane Nunnick, Donna Walsh, Mirjana Sirovica, Shamyla Saddique, Sandeep Nagra, Paul Ferguson, Paul Moss, Ram Malladi
Summary: Immune responses involving HA-1 prime-boost vaccination can induce strong and specific T cell responses in donors or patients, leading to enhanced graft-versus-leukaemia effects. This approach shows promising potential and efficacy in experimental settings.
BRITISH JOURNAL OF HAEMATOLOGY
(2021)
Article
Multidisciplinary Sciences
Taylor Pursell, Jennifer L. Spencer Clinton, Jie Tan, Rongsheng Peng, Paul D. Ling
Summary: EEHV can cause lethal hemorrhagic disease in Asian elephants, and developing a vaccine would significantly improve conservation efforts for this endangered species. Recent studies show that a recombinant virus can induce robust humoral and cell-mediated responses.
Article
Immunology
Rui Dai, Xiaopei Huang, Yiping Yang
Summary: This study demonstrates the critical role of gamma delta T cells in promoting the CD8(+) T cell response to VV infection. Gamma delta T cells are able to present viral antigens and activate CD8(+) T cells, with MyD88 signaling playing a key role in this process. Understanding the regulation of adaptive T cell response by gamma delta T cells may provide insight for the development of more effective vaccine strategies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Gastroenterology & Hepatology
Timothy Donnison, Joey McGregor, Senthil Chinnakannan, Claire Hutchings, Rob J. Center, Pantelis Poumbourios, Paul Klenerman, Heidi E. Drummer, Eleanor Barnes
Summary: This study aims to develop a vaccine that can generate both viral-specific B-cell and T-cell responses concurrently to target multiple HCV genotypes.
Article
Immunology
Keon-Woong Yoon, Ki-Back Chu, Hae-Ji Kang, Min-Ju Kim, Gi-Deok Eom, Fu-Shi Quan
Summary: In this study, it was found that rVV vaccines induced immune responses against Toxoplasma gondii, including high levels of antibody and cellular responses, providing protection against lethal infection.
Article
Biochemical Research Methods
Gwendal Graenicher, Felipe Tapia, Ilona Behrendt, Ingo Jordan, Yvonne Genzel, Udo Reichl
Summary: The study compares different cell retention options for high-yield MVA virus production in perfusion mode, finding that the highest yields can be achieved through a perfusion strategy with working volume expansion after virus infection.
BIOTECHNOLOGY JOURNAL
(2021)
Review
Biotechnology & Applied Microbiology
Gabrielle P. Dailey, Erika J. Crosby, Zachary C. Hartman
Summary: The success of RNA-based vaccines against SARS-CoV-2 has sparked interest in using RNA vaccines to battle cancer, particularly with the use of self-replicating RNA (srRNA) viral vaccine platforms. These platforms have shown immense potential in provoking anti-tumor immunity and offer a promising approach to developing effective cancer vaccines.
CANCER GENE THERAPY
(2023)
Article
Immunology
Ning Yang, Aitor Garcia, Cindy Meyer, Thomas Tuschl, Taha Merghoub, Jedd D. Wolchok, Liang Deng
Summary: Protein or peptide-based subunit vaccines have shown promise in combating cancer or COVID-19. However, weak immune responses can be a concern. This study demonstrates that heat-inactivated MVA can serve as a potent vaccine adjuvant, enhancing T cell responses and antibody production.
Review
Immunology
Mangalakumari Jeyanathan, Sam Afkhami, Alisha Kang, Zhou Xing
Summary: The optimal vaccination strategies for pandemic respiratory viruses must consider vaccine platform, delivery route, and regimens. Developing vaccines for respiratory infections has been challenging, as systemically administered vaccines fail to induce respiratory mucosal immunity. However, studies have shown that respiratory mucosal vaccination is superior to parenteral vaccination in inducing respiratory mucosal immunity. Recent advancements in developing respiratory mucosal vaccines for pathogens such as M. tuberculosis and SARS-CoV-2, including inhaled aerosol delivery of adenoviral-vectored vaccines, have shown significant promise.
CURRENT OPINION IN IMMUNOLOGY
(2023)
Article
Immunology
Maria M. Lorenzo, Alejandro Marin-Lopez, Kevin Chiem, Luis Jimenez-Cabello, Irfan Ullah, Sergio Utrilla-Trigo, Eva Calvo-Pinilla, Gema Lorenzo, Sandra Moreno, Chengjin Ye, Jun-Gyu Park, Alejandro Matia, Alejandro Brun, Juana M. Sanchez-Puig, Aitor Nogales, Walther Mothes, Pradeep D. Uchil, Priti Kumar, Javier Ortego, Erol Fikrig, Luis Martinez-Sobrido, Rafael Blasco
Summary: The COVID-19 pandemic highlights the need for fast responses and reliable technologies for vaccine development. This study reports on the construction and preclinical testing of a recombinant MVA vaccine, with the MVA-Spf vaccine candidate showing higher levels of antibodies, a stronger T cell response, and a higher degree of protection.
Article
Medicine, Research & Experimental
Stephen Boulton, Joanna Poutou, Rida Gill, Nouf Alluqmani, Xiaohong He, Ragunath Singaravelu, Mathieu J. F. Crupi, Julia Petryk, Bradley Austin, Leonard Angka, Zaid Taha, Iris Teo, Siddarth Singh, Rameen Jamil, Ricardo Marius, Nikolas Martin, Taylor Jamieson, Taha Azad, Jean -Simon Diallo, Carolina S. Ilkow, John C. Bell
Summary: In order to provide broader protection against COVID-19, researchers have developed a second-generation vaccinia virus-based vaccine that generates strong antibody and T cell immune responses and can protect mice from viral infection.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2023)
Article
Immunology
Piet Nuijten, Natalie Cleton, Jeroen van der Loop, Birgit Makoschey, Wilco Pulskens, Geert Vertenten
Summary: Intranasal vaccination with live attenuated BRSV and BPIV3 virus strains can provide early protection against bovine respiratory disease in young calves. Vaccination induced gene expression profiles reflecting an antiviral state, early activation of innate immune and antiviral responses, and specific cellular immune pathways. The vaccinated animals were protected against nasal shedding of the challenge virus and clinical symptoms, and gene expression levels returned to values before vaccination.
Article
Multidisciplinary Sciences
Zengzhang Zheng, Wanyan Deng, Yang Bai, Rui Miao, Shenglin Mei, Zhibin Zhang, Youdong Pan, Yi Wang, Rui Min, Fan Deng, Zeyu Wu, Wu Li, Pengcheng Chen, Tianchi Ma, Xiwen Lou, Judy Lieberman, Xing Liu
Meeting Abstract
Hematology
Christa Haase, Karin Gustafsson, Shenglin Mei, Jelena Milosevic, Shu-Chi Yeh, David Sykes, Peter Kharchenko, David T. Scadden, Charles Lin
Article
Oncology
Youmna Kfoury, Ninib Baryawno, Nicolas Severe, Shenglin Mei, Karin Gustafsson, Taghreed Hirz, Thomas Brouse, Elizabeth W. Scadden, Anna A. Igolkina, Konstantinos Kokkaliaris, Bryan D. Choi, Nikolas Barkas, Mark A. Randolph, John H. Shin, Philip J. Saylor, David T. Scadden, David B. Sykes, Peter Kharchenko
Summary: Bone metastases are devastating complications of cancer, particularly common in prostate cancer and refractory to immunotherapy. Analysis of single cells reveals multifaceted immune distortion in metastatic prostate cancer, with overexpression of CCL20 by myeloid cells and appearance of unique macrophages specific to PCa bone metastases. Disruption of the CCL20-CCR6 axis restores T cell reactivity and prolongs animal survival in mice with syngeneic PCa bone metastases, suggesting a targeted approach for relieving local immunosuppression.
Article
Multidisciplinary Sciences
Yiji Liao, Chen-Hao Chen, Tengfei Xiao, Barbara de la Pena Avalos, Eloise Dray, Changmeng Cai, Shuai Gao, Neel Shah, Zhao Zhang, Avery Feit, Pengya Xue, Zhijie Liu, Mei Yang, Ji Hoon Lee, Han Xu, Wei Li, Shenglin Mei, Roodolph S. Pierre, Shaokun Shu, Teng Fei, Melissa Duarte, Jin Zhao, James E. Bradner, Kornelia Polyak, Philip W. Kantoff, Henry Long, Steven P. Balk, X. Shirley Liu, Myles Brown, Kexin Xu
Summary: Drugs that block the activity of the methyltransferase EZH2 have shown potential for treating non-Hodgkin lymphomas with EZH2 gain-of-function mutations. This study reveals that EZH2 inhibitors can also inhibit the growth of castration-resistant prostate cancer (CRPC) cells by blocking the transactivation activity of EZH2 and down-regulating DNA damage repair (DDR) genes. The expression of DDR genes is correlated with EZH2 dependency and cellular sensitivity to EZH2 inhibitors in various types of solid tumors. These findings provide insights into the mechanism of action of EZH2 inhibitors and have implications for combination cancer therapies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Correction
Multidisciplinary Sciences
Wanyan Deng, Yang Bai, Fan Deng, Youdong Pan, Shenglin Mei, Zengzhang Zheng, Rui Min, Zeyu Wu, Wu Li, Rui Miao, Zhibin Zhang, Thomas S. Kupper, Judy Lieberman, Xing Liu
Article
Multidisciplinary Sciences
Adele M. Alchahin, Shenglin Mei, Ioanna Tsea, Taghreed Hirz, Youmna Kfoury, Douglas Dahl, Chin-Lee Wu, Alexander O. Subtelny, Shulin Wu, David T. Scadden, John H. Shin, Philip J. Saylor, David B. Sykes, Peter V. Kharchenko, Ninib Baryawno
Summary: By analyzing ccRCC patient samples using single-cell transcriptomics, a distinct transcriptional signature predictive of metastatic potential and patient survival was identified. Potential therapeutic targets including the CXCL9/CXCL10-CXCR3 axis and the CD70-CD27 axis were highlighted.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Yuqiu Xu, Zhuang Wei, Mei Feng, Dexiang Zhu, Shenglin Mei, Zhongen Wu, Qingyang Feng, Wenju Chang, Meiling Ji, Chenglong Liu, Yuanyuan Zhu, Lian Shen, Fan Yang, Yijiao Chen, Yuxiong Feng, Jianmin Xu, Di Zhu
Summary: This study used single-cell RNA sequencing to investigate the immune cells involved in colorectal cancer liver metastasis. The researchers found that activated B cells were significantly depleted in patients with liver metastasis. They also identified a subtype of B cells called immature plasma cell alpha that was highly correlated with metastasis. Inhibition of the Wnt and TGF-0 pathways in cancer cells promoted the migration of activated B cells. These findings highlight the importance of B cell subpopulations in colorectal cancer liver metastasis and suggest potential strategies for drug development and management.
Article
Multidisciplinary Sciences
Mykhailo Y. Batiuk, Teadora Tyler, Katarina Dragicevic, Shenglin Mei, Rasmus Rydbirk, Viktor Petukhov, Ruslan Deviatiiarov, Dora Sedmak, Erzsebet Frank, Virginia Feher, Nikola Habek, Qiwen Hu, Anna Igolkina, Lilla Roszik, Ulrich Pfisterer, Diego Garcia-Gonzalez, Zdravko Petanjek, Istvan Adorjan, Peter V. Kharchenko, Konstantin Khodosevich
Summary: Schizophrenia is a widespread and complex mental disorder characterized by a reduction in abundance of GABAergic neurons and an increase in principal neurons, especially in the upper cortical layers. Many neuronal subtypes show extensive transcriptomic changes, particularly in upper-layer GABAergic neurons. Transcription factor network analysis reveals the developmental origin of these transcriptomic changes.
Article
Biochemical Research Methods
Christa Haase, Karin Gustafsson, Shenglin Mei, Shu-Chi Yeh, Dmitry Richter, Jelena Milosevic, Raphael Turcotte, Peter Kharchenko, David B. Sykes, David T. Scadden, Charles P. Lin
Summary: Image-seq is a technology that allows single-cell transcriptional data to be obtained from cells isolated from specific spatial locations under image guidance, preserving the spatial information of the cells. It can be used with in situ and in vivo imaging to document the temporal and dynamic history of the cells being analyzed. This technology combines spatial information with highly sensitive RNA sequencing, providing valuable insights into cellular organization and function.
Meeting Abstract
Oncology
Bronte Manouk Verhoeven, Shenglin Mei, Thale Olsen, Karin Gustafsson, Anders Valind, David Gisselsson Nord, Shahrzad Fard, Catharina Hagerling, Peter Kharchenko, Per Kogner, John Inge Johnsen, Ninib Baryawno
PEDIATRIC BLOOD & CANCER
(2022)
Article
Cell Biology
Bronte Manouk Verhoeven, Shenglin Mei, Thale Kristin Olsen, Karin Gustafsson, Anders Valind, Axel Lindstrom, David Gisselsson, Shahrzad Shirazi Fard, Catharina Hagerling, Peter Kharchenko, Per Kogner, John Inge Johnsen, Ninib Baryawno
Summary: Understanding the immune cell composition of human neuroblastoma is important for developing immunotherapeutics. In this study, single-cell RNA sequencing was performed on 19 neuroblastoma samples, revealing a comprehensive landscape of immune cells and identifying potential therapeutic targets and interactions.
CELL REPORTS MEDICINE
(2022)
Meeting Abstract
Oncology
Jorg Otte, Thale Olsen, Shenglin Mei, Polina Kameneva, Bethel Tesfai Embaie, Asa Bjorklund, Anna Johansson, Erik Sundstrom, Tommy Martinsson, Susanne Fransson, Jakob Stenman, Huaitao Cheng, Vasilios Zachariadis, Martin Enge, John Inge Johnsen, Per Kogner, Igor Adameyko, Peter Kharchenko, Ninib Baryawno
PEDIATRIC BLOOD & CANCER
(2022)
Meeting Abstract
Oncology
Ioanna Tsea, Thale Olsen, Jorg Otte, Shenglin Mei, Bethel Tesfai Embaie, Per Kogner, John Inge Johnsen, Ninib Baryawno, Shahrzad Fard
PEDIATRIC BLOOD & CANCER
(2022)
Meeting Abstract
Oncology
Adele Alchahin, Bethel Tesfai Embaie, Shenglin Mei, Bronte Manouk Verhoeven, Thale Olsen, Peter Kharchenko, John Inge Johnsen, Per Kogner, Ninib Baryawno
PEDIATRIC BLOOD & CANCER
(2022)
Meeting Abstract
Oncology
Bethel Tesfai Embaie, Hirak Sarkar, Adele Alchahin, Jorg Otte, Thale Olsen, Shenglin Mei, Peter Kharchenko, Ninib Baryawno
PEDIATRIC BLOOD & CANCER
(2022)