Review
Biochemistry & Molecular Biology
Wan-Ting Meng, Hai-Dong Guo
Summary: Induced pluripotent stem cell (iPSC) therapy holds promise for treating myocardial injuries, with extracellular vesicles being a key mechanism of action. iPSC-derived small extracellular vesicles (iPSCs-sEVs) can transport genetic and protein substances and facilitate interaction between iPSCs and target cells. Recent studies have focused on the therapeutic effect of iPSCs-sEVs in various conditions such as myocardial infarction, myocardial ischemia-reperfusion injury, coronary heart disease, and heart failure. Different methods, including ultracentrifugation, isodensity gradient centrifugation, and size exclusion chromatography, have been used to isolate iPSCs-sEVs for myocardial injury treatment. Administration of iPSCs-sEVs via tail vein injection and intraductal administration is commonly practiced. Comparison of iPSCs-derived sEVs from different species and organs, such as fibroblasts and bone marrow, have been conducted. Furthermore, CRISPR/Cas9 can be utilized to modulate beneficial genes in iPSCs for altering sEV composition and enhancing their abundance and expression diversity. This review provides insights into the strategies and mechanisms of iPSCs-sEVs in treating myocardial injury, serving as a reference for future research and clinical application.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell & Tissue Engineering
Bruno Moises de Matos, Marco Augusto Stimamiglio, Alejandro Correa, Anny Waloski Robert
Summary: Extracellular vesicles (EVs) are nanometric particles that act as intercellular communication tools by enclosing cell-derived bioactive molecules in a lipid bilayer. They play important roles in immune modulation, senescence, and cell proliferation and differentiation. This review article provides an overview of studies using EVs derived from human pluripotent stem cells (hPSC-EVs), focusing on their isolation conditions, characterization, and demonstrated applications, highlighting the significance of hPSC-EVs as potential cell-free therapy products derived from pluripotent stem cells.
WORLD JOURNAL OF STEM CELLS
(2023)
Review
Biochemistry & Molecular Biology
Anna Trzyna, Agnieszka Banas-Zabczyk
Summary: The secretome of ASCs, including exosomes, contains a variety of cytokines and non-coding RNAs that have therapeutic potential in a wide range of diseases. Utilizing ASC secretome as a cell-free therapy shows promise in overcoming limitations of cell-based therapies and improving treatment efficacy.
Article
Cell Biology
Ying Luo, Dunqin Gao, Peng Wang, Cheng Lou, Tong Li, Wenhui Niu, Yingtang Gao
Summary: Extracellular vesicles derived from stem cells have significant potential in disease treatment. Optimizing culture conditions and extraction methods can yield high-quality small extracellular vesicles. The use of 0.5% ED-KSR concentration and continuous collection of culture supernatant for 5 days can maintain cell viability and pluripotent characteristics, leading to biologically active sEVs.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Review
Cell & Tissue Engineering
Ghaidaa Raheem Lateef Al-Awsi, Fahad Alsaikhan, Ria Margiana, Irfan Ahmad, Indrajit Patra, Mazin A. A. Najm, Ghulam Yasin, Iroda Rasulova, Ali Thaeer Hammid, Hamzah H. Kzar, Moaed E. Al-Gazally, Homayoon Siahmansouri
Summary: Breast cancer is the second most frequently diagnosed cancer and the leading cause of cancer death in women. Mesenchymal stem cells (MSCs) are a subgroup of cells that can differentiate into multiple cell types and have been found to have various effects on tumor growth and development. Recent studies have shown that MSCs can both promote and inhibit tumor development. Exosomes (Exo) have emerged as a new mechanism of cell communication, and MSC-derived exosomes (MSCs-Exo) have been found to carry molecules that can affect the proliferation, metastasis, and drug response of breast cancer cells.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Veterinary Sciences
Manon Jammes, Romain Contentin, Frederic Casse, Philippe Galera
Summary: Osteoarthritis is a degenerative disease without a curative treatment. Studying equine OA can help improve horse health and serve as preclinical studies for human medicine. The immunomodulatory and regenerative potentials of mesenchymal stromal cells (MSCs) and their extracellular vesicles (EVs) offer promising acellular therapy options. Optimizing MSC secretome potential and exploring pro-inflammatory environments could enhance MSC therapies for managing OA.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Giuliana Minani Bertolino, Marie Maumus, Christian Jorgensen, Daniele Noel
Summary: Extracellular vesicles (EVs) isolated from induced pluripotent stem cells (iMSCs) show promise in regenerative medicine for the treatment of various diseases. However, the heterogeneity between MSC donors may lead to batch-to-batch variability in the use of EVs as cell-free therapeutic agents.
Article
Cell Biology
Bin Liu, Zhiqing Li, Shi Huang, Biying Yan, Shan He, Fengyuan Chen, Yaxuan Liang
Summary: The study developed a novel strategy to produce and purify AAV-containing exosomes (AAVExo) from AAV-packaging HEK 293T cells. The AAVExo improved gene transfer efficiency in lung cancer cell lines compared to conventional AAV vectors, and showed remarkable resistance to antibody neutralization in vitro and in a lung cancer xenograft rodent model.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chaoran Liu, Yanan Li, Guanghong Han
Summary: This review summarizes the current understanding of the role of extracellular vesicles in periodontal bone remodeling, exploring functional gene expression through comparative analysis of transcriptomic content.
DNA AND CELL BIOLOGY
(2022)
Review
Cell Biology
Zhengyu Zhang, Aime Mugisha, Silvia Fransisca, Qinghuai Liu, Ping Xie, Zizhong Hu
Summary: Retinal diseases, leading causes of vision loss and blindness, involve complex pathogeneses such as angiogenesis, inflammation, immune regulation, fibrous proliferation, and neurodegeneration. Exosomes, as important mediators of cell communication, carry a variety of biomolecules and have the potential to be therapeutically effective in treating retinal diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell & Tissue Engineering
Zhihao Wang, Rob Knight, Phil Stephens, E. M. Ongkosuwito, Frank A. D. T. G. Wagener, Johannes W. Von den Hoff
Summary: Orofacial soft tissue wounds caused by surgery for congenital defects, trauma, or disease frequently occur leading to complications affecting patients' quality of life. Scarring and fibrosis prevent proper skin, mucosa and muscle regeneration during wound repair. This review provides an overview of the putative pitfalls, promises and the future of stem cells and EV therapy, focused on orofacial soft tissue regeneration.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Biochemistry & Molecular Biology
Jinsu Hwang, Sujeong Jang, Choonghyo Kim, Sungjoon Lee, Han-Seong Jeong
Summary: Neurological disorders pose a global health problem, and current pharmacological treatments have side effects. Mesenchymal stem cells (MSCs) have anti-inflammatory, anti-apoptotic, and immunomodulatory properties, and their secreted paracrine factors play a therapeutic role. Exosomes and MSC-derived miRNAs also regulate genes and alleviate neuropathological changes in neurological diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Bocheng Zhang, Xiaoyuan Tian, Zhenan Qu, Jun Hao, Weiguo Zhang
Summary: This study aimed to compare the effects of hypoxia-preconditioned and normoxia-preconditioned MSC-EVs on OA cartilage repair, as well as to investigate the impact of hypoxia on microRNA expression in MSC-EVs. The results demonstrated that hypoxia-preconditioned MSC-EVs exhibited superior effects in enhancing chondrocyte proliferation and migration and suppressing chondrocyte apoptosis compared to normoxia-preconditioned MSC-EVs. Additionally, bioinformatics analysis revealed that hypoxia may promote cartilage repair through the miRNA-18-3P/JAK/STAT or miRNA-181c-5p/MAPK signaling pathway.
Review
Pharmacology & Pharmacy
Zhenghua Li, Zhen Liu, Jiacai Wu, Bin Li
Summary: This review provides an overview of recent advances in naturally occurring mRNA delivery platforms and their biomedical applications, as well as discusses the future perspectives on the clinical translation of cell-derived vesicles.
Article
Engineering, Chemical
Laura Herbst, Ferdinand Groten, Mary Murphy, Georgina Shaw, Bastian Niessing, Robert H. Schmitt
Summary: This study presents an adaptable and modular automation concept for handling the expansion, differentiation, and purification of iPSCs through integration of existing automation technologies, in order to meet the requirements of clinical production.
Article
Automation & Control Systems
Maryam Shariatzadeh, Amit Chandra, Samantha L. Wilson, Mark J. McCall, Lise Morizur, Lea Lesueur, Olivier Chose, Michael M. Gepp, Andre Schulz, Julia C. Neubauer, Heiko Zimmermann, Elsa Abranches, Jennifer Man, Orla O'Shea, Glyn Stacey, Zoe Hewitt, David J. Williams
INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY
(2020)
Review
Neurosciences
Lise Morizur, Elise Herardot, Christelle Monville, Karim Ben M'Barek
MOLECULAR AND CELLULAR NEUROSCIENCE
(2020)
Article
Multidisciplinary Sciences
Marc-Andre Mouthon, Lise Morizur, Lea Dutour, Donovan Pineau, Thierry Kortulewski, Francois D. Boussin
Review
Biochemistry & Molecular Biology
Juliette Duchesne de Lamotte, Anselme Perrier, Cecile Martinat, Camille Nicoleau
Summary: Botulinum neurotoxins (BoNTs) are deadly toxins produced by Clostridium botulinum, but are also used for cosmetic and therapeutic purposes. Engineering BoNTs can expand their applications and improve safety and clinical benefits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Juliette Duchesne de Lamotte, Jerome Polentes, Florine Roussange, Lea Lesueur, Pauline Feurgard, Anselme Perrier, Camille Nicoleau, Cecile Martinat
Summary: This study developed a functional muscle-nerve coculture system using human-induced pluripotent stem cell-derived motor neurons and muscle cells for investigating the sensitivity to botulinum neurotoxins (BoNTs). Optogenetics combined with live calcium imaging was implemented to monitor the impact of BoNTs on synaptic transmission in a human motor endplate model, demonstrating potential for pharmaceutical BoNTs testing and development.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Ekene Anakor, Vanessa Milla, Owen Connolly, Cecile Martinat, Pierre Francois Pradat, Julie Dumonceaux, William Duddy, Stephanie Duguez
Summary: Extracellular vesicles play a crucial role in intercellular communication and their study in Amyotrophic Lateral Sclerosis (ALS) is important. The smaller exosome-like vesicles from ALS patients have negative effects on motor neurons, astrocytes, and myotubes, while vesicles from healthy subjects or larger vesicles from ALS or healthy subjects do not exhibit such effects.
Article
Biochemistry & Molecular Biology
Manoubia Saidani, Annabelle Darle, Margot Jarrige, Helene Polveche, Lina El Kassar, Severine Julie, Sandrine Bessou-Touya, Nathalie Holic, Gilles Lemaitre, Cecile Martinat, Christine Baldeschi, Jennifer Allouche
Summary: Melanocytes play a crucial role in maintaining skin homeostasis and protection, with their dysfunction resulting in various diseases. Cell therapy using autologous melanocytes has been used as a supplementary treatment for hypopigmentary disorders like vitiligo, but limited by the poor proliferative capacity of melanocytes obtained from skin biopsies. Recent advances in human pluripotent stem cells offer the potential to generate melanocytes. In this study, we have developed a well-characterized method to produce functional and proliferative melanocytes, with genetic stability assessed during in vitro culture. Transcriptomic analysis has provided molecular signatures throughout the differentiation process, offering a basis for standardization. Overall, our results offer promising, widely applicable, and long-lasting advancements for pigmentation disorders, creating opportunities for innovative biotherapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Elie Frank, Michel Cailleret, Constantin Nelep, Pascal Fragner, Jerome Polentes, Elise Herardot, Lina El Kassar, Karine Giraud-Triboult, Christelle Monville, Karim Ben M'Barek
Summary: Researchers have optimized the CRISPR/Cas9 editing technique and developed a semi-automated method for isolating edited human pluripotent stem cells (hPSCs). This method, which uses a robotic platform, is faster and more reliable than current manual approaches, greatly improving the efficiency of generating edited hPSCs.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Cell Biology
Noemie Berenger-Currias, Cecile Martinat, Sandrine Baghdoyan
Summary: DM1 is a progressive multisystemic disease caused by the expansion of a CTG repeat tract in the DMPK gene. Different in vitro models, especially human pluripotent stem cell-based models, have significantly contributed to the understanding of DM1 mechanism and development of new therapeutic approaches. These models have led to phase III clinical trials for DM1 treatment.
Review
Biochemistry & Molecular Biology
Morgan Gazzola, Cecile Martinat
Summary: Over the past 20 years, the use of pluripotent stem cells to mimic the complexities of the human neuromuscular junction has been widely studied. This review summarizes the development of in vitro neuromuscular junction models from the differentiation of human embryonic stem cells into motor neurons to the recent neuromuscular organoids. The potential of these models in understanding and treating neuromuscular diseases, as well as their application in drug screening, are also discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Laurent R. Gauthier, Mahasen Saati, Hayet Bensalah-Pigeon, Karim Ben M'Barek, Oscar Gitton-Quent, Romane Bertrand, Didier Busso, Marc-Andre Mouthon, Ada Collura, Marie-Pierre Junier, Herve Chneiweiss, Jose R. Pineda, Francois D. Boussin
SCIENTIFIC REPORTS
(2020)