Review
Chemistry, Applied
Chen Zhou, Haimin Zou, Chengjun Sun, Yongxin Li
Summary: This review highlights the importance of antibiotics in the prevention and treatment of infectious diseases in animals, as well as the threats posed by residual antibiotics to human and animal health. Biosensors, as effective tools for detecting antibiotic residues, have great potential for wide application in different food matrices. Through evaluation of recognition elements and signal amplification techniques, the future development direction of antibiotic detection methods in food is outlined.
Article
Chemistry, Applied
Zhengwei Xiong, Qiang Wang, Yuejie Xie, Ning Li, Wen Yun, Lizhu Yang
Summary: A dual DNA tweezers nanomachine was developed for simultaneous detection of AFB1 and OTA in food samples, achieving high sensitivity and accuracy through the binding of aptamers with their targets to control the fluorescent signals. The method demonstrated satisfactory recoveries and precision, with detection uncertainties within 5% at a 95% confidence level.
Article
Chemistry, Analytical
Yong-Qiang He, Ying Chen, Xian-Zhu Meng, Hong -Chao Yi, Hui-Wen Gu, Xiao-Li Yin
Summary: A visual and smartphone-integrated aptasensor for rapid Staphylococcus aureus determination was developed using Exo III-assisted recycling and hemin/G-quadruplex DNAzymes for signal amplification. The assay quantified the released DNAzymes via Exo III-assisted recycling reaction and analyzed them with a smartphone and UV-Vis spectrophotometer. This method exhibited good analytical performance, low cost, and portability, making it a rapid point-of-care test method with great potential in food safety control.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Zhenli Xu, Jiaqi Wang, Zhijian Jia, Yong-Xiang Wu, Ning Gan, Shaoning Yu
Summary: DNAzyme-driven DNA walkers are an excellent signal amplification strategy for the simultaneous, rapid, and sensitive analysis of food-borne pathogens. A multivalent DNA walker sensor combined with microfluidic chip technology was developed for the simultaneous, rapid, and sensitive analysis of Vibrio parahaemolyticus, Salmonella typhimurium, and Staphylococcus aureus. The sensor showed low detection limits and wide detection ranges, and achieved double amplification through gold stirring rod enrichment and DNA walker. Moreover, by changing the design of the substrate chain, the sensor has the potential to detect other targets, expanding its practical applications.
Review
Environmental Sciences
Kamal Rahimizadeh, Qurat ul ain Zahra, Suxiang Chen, Bao T. Le, Ismat Ullah, Rakesh N. Veedu
Summary: Given the importance of public health, the development of quick, targeted, highly sensitive, and accurate technologies to monitor pathogenic microbes is crucial. Nanomaterial-conjugated aptasensors have advantages in improving sensitivity and specificity, and have potential applications in bioanalysis of food and environmental samples.
ENVIRONMENTAL RESEARCH
(2023)
Article
Chemistry, Analytical
Jing Qian, Yue Liu, Haining Cui, Huiyuan Yang, Mustafa Hussain, Kun Wang, Jie Wei, Lingliang Long, Lijun Ding, Chengquan Wang
Summary: A disposable aptasensing chip has been developed for simultaneous label-free detection of four coexisting toxins in food, showing characteristics of simplicity, easy operation, and multiple toxin recognition.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Jianhao Hu, Zhipeng Shen, Lei Tan, Jun Yuan, Ning Gan
Summary: In this study, a double stirring bars-based signal amplification strategy was demonstrated for simultaneous electrochemical detection of Vibrio parahaemolyticus and Salmonella typhimurium, achieving high sensitivity and cost savings through in-situ amplification and electrochemical detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemistry & Molecular Biology
Li Li, Zhengbo Chen
Summary: In this study, a high specificity, flexibility, and simplicity aptasensor strategy was designed using a triblock DNA probe. It was found that the polyA fragment could assemble on the gold electrode surface via polyA instead of traditional Au-S bonds, leading to improved hybridization stability. The electrochemical aptasensor showed good repeatability, stability, and specificity.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Khalil Abnous, Ali khakshour Abdolabadi, Mohammad Ramezani, Mona Alibolandi, Morteza Alinezhad Nameghi, TaranehSadat Zavvar, Zahra Khoshbin, Parirokh Lavaee, Seyed Mohammad Taghdisi, Noor Mohammad Danesh
Summary: By utilizing signal amplification elements of TdT and CRISPR-Cas12a, a highly sensitive and simple electrochemical aptasensor was developed for cocaine quantification, demonstrating excellent selectivity and potential for diverse applications in analyte determination.
Article
Chemistry, Analytical
Zi-Tao Zhong, Ghazala Ashraf, Wei Chen, Lai-Bo Song, Shu-Jie Zhang, Bo Liu, Yuan-Di Zhao
Summary: Duplex-specific nuclease (DSN) is a promising tool for bioanalysis due to its unique capability to cleave DNA within duplexes. This study proposes a new sensing strategy by coupling DSN with DNA aptamer sequence for diverse analytes detection with signal amplification and cycling. The method shows satisfactory performance in detecting adenosine triphosphate, thrombin, and alpha-fetoprotein.
MICROCHEMICAL JOURNAL
(2022)
Review
Biotechnology & Applied Microbiology
Chitra Padmakumari Kurup, Noor Faizah Mohd-Naim, Minhaz Uddin Ahmed
Summary: Ultra-sensitive biosensors require signal enhancement strategies to achieve low detection limits, with nanotechnology emerging as a strong contender for amplifying signals. Nanomaterials, including gold, carbon, graphene, carbon nanotubes, quantum dots, and metal-organic frameworks, have been utilized as signal amplification components in aptamer-based electrochemical biosensors. These advancements contribute to the development of biosensors for scientific research.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Bei Jin, Biao Ma, Qing Mei, Shujuan Xu, Xin Deng, Yi Hong, Jiali Li, Hanyue Xu, Mingzhou Zhang
Summary: In this study, a rapid and visual europium nanoparticle (EuNP)-based lateral flow strip biosensor (LFSB) combined with recombinase polymerase amplification (RPA) was developed for the simultaneous quantitative detection of five foodborne pathogenic bacteria. The ultrasensitive LFSBs exhibited high sensitivity and good specificity, with a recovery rate of 90.6-101.6% in artificial contamination experiments. This study not only has the potential for widespread application in resource-poor areas but also provides insights for multiple detection in the field.
Article
Chemistry, Analytical
Jie Yang, Hongmei Zeng, Yaqin Chai, Ruo Yuan, Hongyan Liu
Summary: An ultra-sensitive signal quenched photoelectrochemical (PEC) aptasensor was constructed for dibutyl phthalate (DBP) detection, using a target-induced cruciform DNA structure as signal amplifier and gC3N4/SnO2 composite as signal indicator. The designed cruciform DNA structure exhibited high signal amplification efficiency due to reduced steric hindrance, and the fabricated PEC biosensor showed a low detection limit of 0.3 fM for DBP in a wide linear range of 1 fM to 1 nM. This work provided a novel nucleic acid signal amplification approach for enhancing the sensitivity of PEC sensing platforms for the detection of phthalates (PAEs)-based plasticizer.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Mengni Sun, Na Ma, Hanxing Shi, Ling-Zhi Cheong, Wenge Yang, Zhaohui Qiao
Summary: A multivalent aptamer based on hybridization chain reaction (HCR) was designed as an effective signal amplifier for the sensitive detection of Salmonella. The multi-Apt exhibited a 33-fold enhancement in binding affinity and faster reaction kinetics compared to the monovalent aptamer (mono-Apt). By utilizing the HCR scaffold as a signal carrier, the proposed HCR-based multi-Apt amplifier could detect Salmonella as low as 7 cfu mL-1 with a broad detection range of 10 to 107 cfu mL-1. It also demonstrated excellent specificity against target bacteria and performed well on the detection of spiked food samples, showing great potential in practical Salmonella detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Xinmei Song, Huahui Gao, Ruo Yuan, Yun Xiang
Summary: This study developed a label-free and aptamer-based sensitive carcinoembryonic antigen (CEA) detection method, which achieved high sensitivity through cyclic cleavage and catalytic reduction.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Food Science & Technology
Juanjuan Du, Changyu Zhou, Qiang Xia, Ying Wang, Fang Geng, Jun He, Yangying Sun, Daodong Pan, Jinxuan Cao
Summary: This study evaluated the effects of fibrin addition on the water-holding capacity, texture, rheological properties, interactions, and microstructure of myofibrillar proteins (MP) gel during the heat-induced process. Results showed that the addition of fibrin improved the whiteness, water-holding capacity, hardness, storage modulus, and loss modulus of the MP gel. The incorporation of fibrin also led to alterations in protein conformation and increased nonspecific association and hydrophobic interactions, contributing to enhanced gelling and rheological properties. Additionally, scanning electron microscopy revealed a more ordered and homogeneous gel network structure with the addition of fibrin. Overall, fibrin enhanced the functionality of MP, suggesting potential benefits for gel-type meat products.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Zhaoyu Ma, Mingkun Yang, Mohamed Frahat Foda, Kai Zhang, Shuting Li, Huageng Liang, Yanli Zhao, Heyou Han
Summary: By delivering vitamin C to the tumor site using responsive lipid nanoparticles, oxidative toxicity and polarization of M1 macrophages can be induced, promoting the infiltration of cytotoxic T lymphocytes in the tumor microenvironment and enhancing immune checkpoint blocking therapy. This approach shows better immune response than using clinical Anti-PD-L1 alone, providing a new therapeutic application for patients with clinically insensitive immunity.
Article
Chemistry, Multidisciplinary
Kai Zhang, Zhaoyu Ma, Shuting Li, Weiyun Zhang, Mohamed Frahat Foda, Yanli Zhao, Heyou Han
Summary: In this study, a biomimetic nanoflower platform with platelet membrane modification was developed for targeted delivery of anticoagulant drugs to treat both cancer and thrombosis, showing significant therapeutic efficacy and prolonged survival time.
Article
Chemistry, Analytical
Zhenli Xu, Jiaqi Wang, Zhijian Jia, Yong-Xiang Wu, Ning Gan, Shaoning Yu
Summary: DNAzyme-driven DNA walkers are an excellent signal amplification strategy for the simultaneous, rapid, and sensitive analysis of food-borne pathogens. A multivalent DNA walker sensor combined with microfluidic chip technology was developed for the simultaneous, rapid, and sensitive analysis of Vibrio parahaemolyticus, Salmonella typhimurium, and Staphylococcus aureus. The sensor showed low detection limits and wide detection ranges, and achieved double amplification through gold stirring rod enrichment and DNA walker. Moreover, by changing the design of the substrate chain, the sensor has the potential to detect other targets, expanding its practical applications.
Article
Food Science & Technology
Aiyue Xiang, Ying Wang, Baoguo Sun, Xinglian Xu, Guanghong Zhou, Changyu Zhou, Fang Geng, Daodong Pan, Jinxuan Cao
Summary: Excessive bitterness is a common problem in the dry-cured ham industry, and it is related to the degradation of structural proteins. This study investigated the sensory attributes and peptide degradation of both premium and defective hams. The results showed that defective hams had higher bitterness intensities and lower richness intensities compared to premium hams. The activities of exopeptidases were also different between the two types of hams. Unique peptide fragments and cleavage sites were identified in defective hams, which were associated with the excessive accumulation of peptides and the development of excessive bitterness. This study provides a theoretical basis for regulating bitterness defects in dry-cured ham in the future.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemical Research Methods
Binglin Mou, Chengyi Zuo, La Chen, Hongzhen Xie, Wentian Zhang, Qiqin Wang, Luhong Wen, Ning Gan
Summary: In this study, a novel nitrogen and sulfur co-doped carbon dot (N, S-CD) was synthesized and used as a QuEChERS clean-up reagent to reduce matrix interferences in the determination of insecticides in vegetables. A portable mass spectrometer (mu-MS) was employed to directly determine neonicotinoids, carbamates, and benzopyrazole insecticides in the pretreated samples. The N,S-CD mu-MS method exhibited effective clean-up performance with satisfactory matrix effects, recoveries, and linear ranges.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Multidisciplinary
Shuting Li, Kai Zhang, Zhaoyu Ma, Weiyun Zhang, Zhiyong Song, Wenjing Wang, Heyou Han
Summary: This study proposes a platelet-mimetic nanoplatform for noninvasive thrombolysis and anticoagulation. By delivering hirudin to the thrombus site and utilizing hyperthermia and reactive oxygen species, thrombolysis can be achieved without the risk of recurrence.
Review
Chemistry, Inorganic & Nuclear
Xiao Xiao, Yizhong Shen, Xin Zhou, Baoguo Sun, Ying Wang, Jinxuan Cao
Summary: Since hydrogen sulfide (H2S) level is a crucial indicator for food safety, it is important to enhance the monitoring accuracy of H2S levels in food production. Fluorescence assays, with their simplicity, convenience, high accuracy, low cost, and real-time capabilities, are considered as one of the most promising tools for H2S monitoring. The progress of fluorescence assays, aided by nanotechnology, has led to the development of innovative nanotechnology-driven fluorescence assays widely used for monitoring H2S levels in food-related matrices.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Materials Science, Multidisciplinary
Kai Zhang, Yuying Long, Zhaoyu Ma, Shuting Li, Yanli Zhao, Heyou Han
Summary: In this study, an engineered artificial nanoplatelet (MOAP) was developed to target thrombus at the residual tumor site and release drugs to inhibit immune checkpoint and reverse the tumor immunosuppressive microenvironment. The MOAP showed a good anti-tumor effect, inhibiting tumor recurrence and lung metastasis after surgery.
Article
Chemistry, Analytical
Bingqian Li, Shengrong Yu, Rong Feng, Zhiling Qian, Kangdi He, Guo-Jiang Mao, Yuting Cao, Keqi Tang, Ning Gan, Yong-Xiang Wu
Summary: Bioimaging, including fluorescence imaging, is widely used in modern medicine. However, one-photon fluorescence imaging has limitations such as low tissue penetration depth and low spatiotemporal resolution. Two-photon fluorescence imaging can overcome these limitations, but the complexity of organisms can affect the accuracy of results. Fluorescence lifetime imaging (FLIM) is an intrinsic property of materials and independent of material concentration and fluorescence intensity, making it a promising alternative. By combining silica-coated gold nanoclusters (AuNCs@SiO2) with nucleic acid probes, a dual-mode nanoprobe platform for two-photon and FLIM imaging of intracellular miRNA-21 was constructed. The dual-mode nanoprobe exhibited high sensitivity and selectivity, with potential applications in miRNA-based early detection and therapy.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Hang You, Ming Wang, Shuai Wang, Jie Xu, Shuhao Hu, Tianhua Li, Zhenzhong Yu, Dianping Tang, Ning Gan
Summary: This research developed an ultrasensitive and specific Phage@DNAzyme signal probe for the rapid and on-site detection of virulent foodborne pathogens. The probe efficiently catalyzed a click reaction to generate a visual fluorescent signal, allowing for the quantification of target concentrations using a smartphone. The method demonstrated satisfactory sensing performances for Escherichia coli O157:H7 and Salmonella typhimurium, providing a new approach for trace level detection of pathogenic strains in foods.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Chuanhu Han, Yuanrong Zheng, Changyu Zhou, Yiqi Liang, Siqiang Huang, Yangying Sun, Jinxuan Cao, Jun He, Francisco J. Barba, Jiqiang Liu, Daodong Pan, Qiang Xia
Summary: To meet the demand for protein in underdeveloped countries, the extraction of protein from inexpensive by-products is crucial. However, animal-derived protein isolate often has off-flavors that hinder the processability of proteins due to the coextraction of off-flavor components. This study investigated the effects of UHP pretreatment on the interaction between off-flavor compounds and duck liver protein (DLp) and demonstrated the tunable interaction processes of off-flavor components with matrix proteins.
Article
Chemistry, Applied
Changyu Zhou, Xueyi Wu, Daodong Pan, Qiang Xia, Yangying Sun, Fang Geng, Jinxuan Cao
Summary: This study investigated the effects of Staphylococcus inoculation on protein degradation and taste of dry-cured bacon. It was found that co-inoculation of Staphylococcus xylosus and Staphylococcus vitulinus improved the taste attributes of the bacon. High residual activities of proteases accelerated protein degradation, and quantitative proteomic analysis revealed down-regulated proteins in the co-inoculated group. Morphological changes of structural proteins were confirmed by atomic force microscopy. The accumulation of glutamic acid, alanine, and lysine was identified as the main contributor to the taste improvement.
Article
Chemistry, Applied
Qiaoyan Wang, Changyu Zhou, Qiang Xia, Daodong Pan, Lihui Du, Jun He, Yangying Sun, Fang Geng, Jinxuan Cao
Summary: The effect of pH treatments on the coacervation mechanism of carrageenan and fibrinogen hydrolysates was investigated. Hydrogels derived from the hydrolysis of alcalase showed the best properties at pH 7.0. These hydrogels had the highest gel strength, best viscoelasticity, and smaller pore sizes. They also showed strong antioxidant capacity and improved preservation times in meat samples.
FOOD HYDROCOLLOIDS
(2024)
Article
Nanoscience & Nanotechnology
Shuting Li, Zhaoyu Ma, Kai Zhang, Weiyun Zhang, Zhiyong Song, Wenjing Wang, Ximiao Yu, Heyou Han
Summary: The study successfully utilized acid-labile metal-organic frameworks as nano-carriers to efficiently mineralize hyaluronidase and encapsulate Ag2S nanodots, resulting in nanocomposites that maintained enzyme activity, altered in size for prolonged circulation, and effectively delivered cargo to the tumor site. This innovative strategy offers a promising approach for NIR-II multimodal imaging-guided PTT of deep tumors by decreasing nanoparticle size and degrading dense extracellular matrix.
ACS APPLIED MATERIALS & INTERFACES
(2022)
