Article
Biochemistry & Molecular Biology
Kaiyi Zhou, Meng Yuan, Jiabao Sun, Feixu Zhang, Xiaoying Zong, Zhanao Li, Dingyue Tang, Lichen Zhou, Jing Zheng, Xiao Xiao, Xia Wu
Summary: Phosphodiesterase-5 inhibitor promotes AAV9 transduction and enhances gene therapy efficacy. The study demonstrates that the inhibitor improves AAV9 transduction, suppresses immune response against AAV9, and enhances the efficacy of gene therapy.
Article
Neurosciences
Ute Haeussler, Joao Neres, Catherine Vandenplas, Caroline Eykens, Irena Kadiu, Carolin Schramm, Renaud Fleurance, Phil Stanley, Patrice Godard, Laurane de Mot, Jonathan van Eyll, Klaus-Peter Knobeloch, Carola A. Haas, Stefanie Dedeurwaerdere
Summary: Structural epilepsies display complex immune activation signatures, but the specific neuroinflammatory pathways driving the pathology are unclear. Transcriptome studies in patients with mesial temporal lobe epilepsy (mTLE) identified dysregulation of transforming growth factor beta, interferon alpha/beta, and nuclear factor erythroid 2-related factor 2 pathways. However, in a mouse model, lack of Usp15 did not significantly affect severity of status epilepticus or the expression of inflammatory pathways.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Orthopedics
G. P. Bensen, A. C. Rogers, V. P. Leifer, R. R. Edwards, T. Neogi, A. M. Kostic, A. D. Paltiel, J. E. Collins, D. J. Hunter, J. N. Katz, E. Losina
Summary: This study examined the cost-effectiveness of adding gabapentin to knee osteoarthritis care. The results showed that incorporating gabapentin into treatment did not offer good value.
OSTEOARTHRITIS AND CARTILAGE
(2023)
Article
Oncology
Jia Huang, Qiulin Huang, Rong Tang, Guodong Chen, Yiwei Zhang, Rongfang He, Xuyu Zu, Kai Fu, Xiuda Peng, Shuai Xiao
Summary: The study compared outcomes of right hemicolectomy (RHC) and partial colectomy (PC) for mucinous adenocarcinoma (MAC) in right-sided colon cancer (RCC) patients. Results showed MAC group had poorer overall survival but similar cancer-specific survival compared to the AC group, with no clear benefit of RHC over PC in terms of long-term survival. This study provides evidence for treatment selection in MAC of RCC, with implications for individualized management of colon cancer patients.
FRONTIERS IN ONCOLOGY
(2021)
Article
Obstetrics & Gynecology
Olivia J. Carpinello, Samad Jahandideh, Meghan Yamasaki, Micah J. Hill, Alan H. DeCherney, Natalie Stentz, Kimberly S. Moon, Kate Devine
Summary: This study compared clinical and ongoing pregnancy outcomes in ovulatory women undergoing therapeutic donor insemination through natural cycle IUI and ovarian stimulation IUI. The results showed that ovarian stimulation led to higher clinical and ongoing pregnancy rates compared to natural cycle, but also significantly increased the risk of ongoing multiple gestations.
FERTILITY AND STERILITY
(2021)
Review
Dermatology
Shreya Singh, Anant Patil, Nika Kianfar, Anna Waskiel-Burnat, Lidia Rudnicka, Rodney Sinclair, Mohamad Goldust
Summary: The article reviews the efficacy and safety of using different concentrations of minoxidil for the treatment of androgenetic alopecia, aiming to identify the most effective concentration range.
CLINICAL AND EXPERIMENTAL DERMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sara Dominguez, Eugene Varfolomeev, Robert Brendza, Kim Stark, Joy Tea, Jose Imperio, Hai Ngu, Timothy Earr, Oded Foreman, Joshua D. Webster, Amy Easton, Domagoj Vucic, Baris Bingol
Summary: Genetic inactivation of RIP1 kinase activity did not benefit disease progression in SOD1-Tg mice, while inhibition of kinase activity may provide benefit in acute injury models.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Biotechnology & Applied Microbiology
Tyler B. Johnson, Katherine A. White, Jon J. Brudvig, Jacob T. Cain, Logan Langin, Melissa A. Pratt, Clarissa D. Booth, Derek J. Timm, Samantha S. Davis, Brandon Meyerink, Shibi Likhite, Kathrin Meyer, Jill M. Weimer
Summary: CLN8 disease is a rare neurological disorder caused by mutations in the CLN8 gene, with no current cure available. Research has shown promising results in using gene therapy to significantly improve disease symptoms and prolong lifespan in animal models of the disease.
Article
Cell Biology
Lee J. Martin, Mark V. Niedzwiecki, Margaret Wong
Summary: Therapeutic hypothermia was found to delay disease onset and reduce functional decline in a transgenic mouse model of ALS. Cooling had protective effects in the CNS and skeletal muscle, targeting mitochondria and extending lifespan in ALS mice.
Review
Chemistry, Multidisciplinary
C. Bastiancich, E. Bozzato, I. Henley, B. Newland
Summary: A systematic analysis of studies shows that local drug delivery is more effective in treating glioblastoma compared to systemic administration, with benefits in terms of median survival, tumor volume, and adverse events. The evidence supports the further development of drug delivery systems for local therapeutic delivery to brain cancers like GBM.
JOURNAL OF CONTROLLED RELEASE
(2021)
Review
Oncology
Darius Khadembaschi, Mariam Jafri, Prav Praveen, Sat Parmar, Omar Breik
Summary: This study investigated the survival benefit of neoadjuvant chemotherapy (neoCTx) for maxillofacial osteosarcoma (MFOS). The results showed that neoCTx did not have a consistent survival benefit compared to surgery as the primary treatment modality in the management of MFOS.
Article
Pharmacology & Pharmacy
Edilaine S. Xavier, Rafael L. de Souza, Vinicius C. Rodrigues, Camila O. Melo, Daniel B. Roquini, Bruna L. Lemes, Polrat Wilairatana, Elquio E. Oliveira, Josue de Moraes
Summary: With praziquantel as the only available drug for schistosomiasis, there is an urgent need for new antischistosomal agents. This study explored the potential of carvacrol-loaded nanoparticles as a treatment for schistosomiasis and found it to be more effective than praziquantel in reducing worm burden and egg production in infected mice.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Otorhinolaryngology
Suleyman Emre Karakurt, Mehmet Ali Cetin, Emre Apaydin, Aykut Ikinciogullari, Serdar Ensari, Haci Huseyin Dere
Summary: The study aimed to evaluate the additional benefits of turbinate outfracture in combination with radiofrequency ablation for the treatment of inferior turbinate hypertrophy. The results showed that this combination treatment significantly improved both objective and subjective parameters, providing benefits in alleviating nasal obstruction.
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
(2021)
Article
Plant Sciences
Yingying Lu, Ruth Jeffers, Anakha Raju, Tamara Kenny, Evangeline Ratchanniyasamu, Wieland Fricke
Summary: The study aimed to investigate the impact of night-time transpiration on wheat plants under salt stress. Salt stress significantly decreased transpiration and leaf elongation rates during the day and night. Night-time transpirational water loss mostly occurred through stomata for respiration. However, it was found that night-time transpiration did not affect the tolerance to salt stress in wheat.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Cardiac & Cardiovascular Systems
Dong Lin, Yequn Chen, Jian Yong, Shiwan Wu, Yan Zhou, Weiping Li, Xuerui Tan, Ruisheng Liu
Summary: This study found that the use of low-dose rivaroxaban and low-intensity warfarin in Asian patients with atrial fibrillation can provide survival benefits, although the effects are smaller than those observed with recommended optimal anticoagulation.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Clinical Neurology
Wenting Guo, Haibo Wang, Arun Kumar Tharkeshwar, Julien Couthouis, Elke Braems, Pegah Masrori, Evelien Van Schoor, Yannan Fan, Karan Ahuja, Matthieu Moisse, Maarten Jacquemyn, Rodrigo Furtado Madeiro da Costa, Madhavsai Gajjar, Sriram Balusu, Tine Tricot, Laura Fumagalli, Nicole Hersmus, Rekin's Janky, Francis Impens, Pieter Vanden Berghe, Ritchie Ho, Dietmar Rudolf Thal, Rik Vandenberghe, Muralidhar L. Hegde, Siddharthan Chandran, Bart De Strooper, Dirk Daelemans, Philip Van Damme, Ludo Van den Bosch, Catherine Verfaillie
Summary: In this study, we identified NEK6 as a novel therapeutic target for C9orf72 FTD/ALS by performing a kinome-wide CRISPR/Cas9 knock-out screen in human induced pluripotent stem cell-derived cortical neurons. NEK6 was found to regulate poly(PR)-mediated p53-related DNA damage.
ALZHEIMERS & DEMENTIA
(2023)
Article
Neurosciences
Maxim De Schaepdryver, Pegah Masrori, Philip Van Damme, Koen Poesen
Summary: The aim of this study was to investigate the impact of measuring neurofilament levels in cerebrospinal fluid (CSF) before referral to a neuromuscular reference center (NMRC) on the diagnostic delay in patients with amyotrophic lateral sclerosis (ALS). The results showed that measuring neurofilament levels in CSF before referral shortened the diagnostic delay in specific cases, but this effect disappeared when other covariates were taken into account.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Clinical Neurology
Jonas Van Lent, Leen Vendredy, Elias Adriaenssens, Tatiana Da Silva Authier, Bob Asselbergh, Marcus Kaji, Sarah Weckhuysen, Ludo van den Bosch, Jonathan Baets, Vincent Timmerman
Summary: Charcot-Marie-Tooth disease (CMT) is the most common inherited disorder of the peripheral nervous system (PNS), with CMT1A accounting for 40-50% of cases. This study presents an organoid model derived from induced pluripotent stem cells that contains various cell types of the PNS, including myelinating human Schwann cells. They used this model to study disease signatures of CMT1A and found that downregulating PMP22 expression can ameliorate the myelin defects in CMT1A-organoids.
Article
Clinical Neurology
Sien Hilde Van Daele, Matthieu Moisse, Joke J. F. A. van Vugt, Ramona A. J. Zwamborn, Rick van der Spek, Wouter van Rheenen, Kristel Van Eijk, Kevin Kenna, Philippe Corcia, Patrick Vourc'h, Philippe Couratier, Orla Hardiman, Russell McLaughin, Marc Gotkine, Vivian Drory, Nicola Ticozzi, Vincenzo Silani, Antonia Ratti, Mamede de Carvalho, Jesus S. Mora Pardina, Monica Povedano, Peter M. Andersen, Markus Weber, Nazli A. Basak, Chris Shaw, Pamela J. Shaw, Karen E. Morrison, John E. Landers, Jonathan D. Glass, Michael A. van Es, Leonard H. van den Berg, Ammar Al-Chalabi, Jan Veldink, Philip Van Damme
Summary: With the development of gene therapies, there is an increasing demand for gene testing in amyotrophic lateral sclerosis (ALS). However, large studies exploring genetic variation in sporadic ALS (sALS) are still limited. This study aimed to characterize genetic variability in ALS genes and identify pathogenic variants using whole genome sequencing data from a large cohort of sALS patients. The study identified pathogenic and likely pathogenic variants in 11.13% of ALS patients in 38 known ALS genes. The findings highlight the complexity of genetic architecture in sALS and the need for further research in gene testing for ALS.
Article
Clinical Neurology
Alberto Catanese, Sandeep Rajkumar, Daniel Sommer, Pegah Masrori, Nicole Hersmus, Philip Van Damme, Simon Witzel, Albert Ludolph, Ritchie Ho, Tobias M. Boeckers, Medhanie Mulaw
Summary: Catanese et al. used a multiomics approach to study ALS, identifying a mutation-independent disease signature and providing insights into the convergent pathomechanisms of different mutations. This work contributes to our understanding of ALS by revealing common transcriptional and epigenetic alterations in the disease.
Article
Medicine, Research & Experimental
Pierre Lemaitre, Samar H. K. Tareen, Emanuela Pasciuto, Loriana Mascali, Araks Martirosyan, Zsuzsanna Callaerts-Vegh, Suresh Poovathingal, James Dooley, Matthew G. Holt, Lidia Yshii, Adrian Liston
Summary: Inflammation and neurodegeneration are common pathological outcomes during aging. Recent studies have shown that T cells accumulate in the brain with age and contribute to inflammatory processes leading to cognitive decline. Researchers have successfully prevented neurological decline in aging mice by delivering IL2 specifically to the brain, restoring cognitive function and spatial learning ability.
EMBO MOLECULAR MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Matthew G. Holt
Summary: Astrocytes, a type of glial cells, play an active role in the synaptic life cycle, including synapse formation, maturation, homeostasis maintenance, and modulation of transmission. Recent studies have revealed unexpected heterogeneity in the development and function of astrocytes, suggesting their potential match with neurons to support local circuits. Therefore, understanding astrocyte heterogeneity and its implications is crucial for comprehending brain function.
ESSAYS IN BIOCHEMISTRY
(2023)
Article
Gastroenterology & Hepatology
Dieudonne Buh Kum, Hannah Vanrusselt, Abel Acosta Sanchez, Valerio Taverniti, Eloi R. Verrier, Thomas F. Baumert, Cheng Liu, Jerome Deval, Nikky Corthout, Sebastian Munck, Leonid Beigelman, Lawrence M. Blatt, Julian A. Symons, Pierre Raboisson, Andreas Jekle, Sandrine Vendeville, Yannick Debing
Summary: This study uncovers a novel mechanism of action for CAM-As in the treatment of chronic hepatitis B, where HBc aggregation induces cell death, leading to hepatocyte proliferation and loss of covalently closed circular DNA or its equivalent, possibly assisted by an induced innate immune response.
Article
Cell Biology
Joke De Vocht, Donatienne Van Weehaeghe, Fouke Ombelet, Pegah Masrori, Nikita Lamaire, Martijn Devrome, Hilde Van Esch, Mathieu Moisse, Michel Koole, Patrick Dupont, Koen Van Laere, Philip Van Damme
Summary: This study examined the impact of ALS-causing gene mutations on cerebral glucose metabolism using genetic testing and FDG PET imaging. The results showed distinctive differences in glucose metabolism patterns between C9orf72-ALS patients and sporadic ALS patients, particularly in specific regions of the brain.
Article
Neurosciences
Jimmy Beckers, Arun Kumar Tharkeshwar, Laura Fumagalli, Matilde Contardo, Evelien Van Schoor, Raheem Fazal, Dietmar Rudolf Thal, Siddharthan Chandran, Renzo Mancuso, Ludo Van Den Bosch, Philip Van Damme
Summary: In this study, iPSC-derived MNs from C9orf72-ALS patients and isogenic control lines were used to investigate the underlying mechanisms of autophagy-lysosome pathway dysregulation. The results showed that C9orf72 loss-of-function had minimal influence on autophagy-lysosome pathway phenotypes, but primarily caused impairment in endosome maturation. Moreover, increased phosphorylation of TBK1 at S172 was observed in MNs derived from C9orf72-ALS patients.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2023)
Article
Cell Biology
Vincent E. Provasek, Manohar Kodavati, Wenting Guo, Haibo Wang, Istvan Boldogh, Ludo Van Den Bosch, Gavin Britz, Muralidhar L. Hegde
Summary: This study examined the impact of FUS gene mutations on the transcriptome of induced pluripotent stem cells and their differentiated motor neurons. The mutations were found to significantly alter the expression profiles of mRNAs and lncRNAs, and several key differentially regulated target pairs were identified. Functional enrichment analyses revealed potential pathways associated with neuronal development and carcinogenesis. The study also highlighted the associations between RNA metabolism, lncRNA regulation, and DNA damage repair. These findings provide insights into the molecular mechanisms underlying ALS-associated FUS mutations and potential therapeutic targets for ALS treatment.
Article
Cell Biology
Carola Torazza, Francesca Provenzano, Elena Gallia, Maria Cerminara, Matilde Balbi, Tiziana Bonifacino, Sara Tessitore, Silvia Ravera, Cesare Usai, Ilaria Musante, Aldamaria Puliti, Ludo Van Den Bosch, Paymaan Jafar-nejad, Frank Rigo, Marco Milanese, Giambattista Bonanno
Summary: This study demonstrates that downregulating mGluR5 expression can alleviate the reactive response and neurotoxicity of SOD1(G93A) astrocytes towards motor neurons, suggesting that mGluR5 may be a promising therapeutic target in ALS.
Editorial Material
Clinical Neurology
Rita Sattler, Bryan J. Traynor, Janice Robertson, Ludo Van den Bosch, Sami J. Barmada, Clive N. Svendsen, Matthew D. Disney, Tania F. Gendron, Philip C. Wong, Martin R. Turner, Adam Boxer, Suma Babu, Michael Benatar, Michael Kurnellas, Jonathan D. Rohrer, Christopher J. Donnelly, Lynette M. Bustos, Kendall Van Keuren-Jensen, Penny A. Dacks, Marwan N. Sabbagh
Summary: The summit highlighted the role of the C9ORF72 gene in FTD and ALS, covering disease mechanisms, therapeutic strategies, and biomarkers. Collaborative efforts aimed to break down existing disease silos and proposed composite endpoints for evaluating treatments covering clinical symptoms.
NEUROLOGY AND THERAPY
(2023)
Meeting Abstract
Clinical Neurology
Pegah Masrori, Baukje Bijnens, Kristofer Davie, Suresh Kumar Poovathingal, Annet Storm, Nicole Hersmus, Laura Fumagalli, Ludo Van Den Bosch, Mark Fiers, Dietmar Thal, Renzo Mancuzo, Philip Van Damme