Functional composition and antioxidant property of crude polysaccharides from the fruiting bodies of Lentinus squarrosulus

Lentinus squarrosulus (Hed Khon Khao) is a source of bioactive polysaccharides. Three L. squarrosulus crude polysaccharides (LSPs) were subjected to cold water (LSP-CP), hot water (LSP-HP), and aqueous alkaline (LSP-AP) extractions, and their functional compositions and radical scavenging activities were compared. Synchrotron radiation FTIR (SR-FTIR) spectra and PCA plot analysis in the bio-regions (4000-400 cm(-1)) revealed that functional composition LSPs differ significantly (P<0.05). All LSPs had lipids, protein, and polysaccharides such as -glucan. The major monosaccharides in LSP-CP and LSP-HP are d-galactose, d-glucose, and d-mannose at different proportions, while LSP-AP contained mainly d-glucose. Also, fucose and xylose were present in all the LSPs. LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 +/- 0.42% at 3 mg/mL, 79.16 +/- 1.43% at 3 mg/mL and 65.26 +/- 1.74% at 5 mg/mL, whiles on 2,2 ' -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 +/- 0.16% at 3 mg/mL, 97.42 +/- 0.76% at 3 mg/mL and 47.24 +/- 0.045% at 5 mg/mL, respectively. The results showed that LSP-CP and LSP-HP are good ABTS scavengers, whiles LSP-AP is poor ABTS scavenger. In overall, LSPs consist of essential functional compositions and could be used as natural antioxidants. This exploitation of fungal fruiting body extracts increased the potential use of L. squarrosulus in food and medicinal industries.

Automated summary

Lentinus squarrosulus (Hed Khon Khao) is a source of bioactive polysaccharides. Three types of crude polysaccharides extracted from L. squarrosulus showed differences in functional compositions and radical scavenging activities. LSP-CP and LSP-HP exhibited good ABTS scavenging abilities, while LSP-AP showed poor performance as an ABTS scavenger.