Article
Biotechnology & Applied Microbiology
Jaehyun Cha, Hyeonseon Bak, Inchan Kwon
Summary: Researchers have discovered a pair of oxygen-tolerant enzymes that can convert hydrogen and carbon dioxide gases into formate, overcoming the issue of enzyme damage caused by the presence of oxygen in the cheap sources.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Seung Kyou You, Hyeon Min Park, Myeong-Eun Lee, Young Jin Ko, Dong-Hyeuk Hwang, Jun Won Oh, Sung Ok Han
Summary: Metabolic engineering of non-photosynthetic microorganisms to increase the utilization of CO2 has been shown to significantly increase the production of fatty acids in the oleaginous yeast Yarrowia lipolytica. The introduction of a CO2 utilization pathway resulted in a 1.5-fold increase in fatty acid production and a 11.6% increase in linoleic acid composition compared to the wild-type strain, demonstrating the potential for developing efficient and eco-friendly processes utilizing CO2.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Physical
Mihai-Cristian Fera, Rita R. Manuel, Ines A. C. Pereira, Jose M. Abad, Antonio L. De Lacey, Marcos Pita
Summary: The electrochemical reduction of CO2 through redox enzymes is a promising method to reduce global CO2 concentrations. In this study, the enzyme Desulfovibrio vulgaris Hildenborough FdhAB formate dehydrogenase was effectively immobilized on MWCNTs-modified low density graphite electrodes using electrostatic interactions and protected with polyethyleneimine (PEI) polymer. The synergistic effect of MWCNTs and PEI resulted in high catalytic current densities and long-term operational stability.
Article
Microbiology
Xin Zhang, Jiyu Xin, Zhiguo Wang, Wenping Wu, Yutong Liu, Zhenzhen Min, Yueyong Xin, Bing Liu, Jun He, Xingwei Zhang, Xiaoling Xu
Summary: This study determined the structure of full-length MCR from the photosynthetic green non-sulfur bacterium Roseiflexus castenholzii and elucidated the catalytic mechanisms using a combination of molecular dynamics simulations and enzymatic analyses. These findings provide a structural and mechanistic basis for enzyme engineering and biosynthetic applications of the 3-HP carbon fixation pathways.
Article
Biotechnology & Applied Microbiology
Jaehyun Cha, Jinhee Lee, Byoung Wook Jeon, Yong Hwan Kim, Inchan Kwon
Summary: This study proposes a CO2 hydrogenation system that utilizes hydrogenase from Ralstonia eutropha H16 (ReSH) and formate dehydrogenase from Methylobacterium extorquens AM1 (MeFDH1) to efficiently convert carbon dioxide in industrial flue gas into formate via a nicotinamide adenine dinucleotide-dependent cascade reaction. The enzyme system is tolerant to inhibitors such as carbon monoxide and oxygen, and it can utilize diverse sources of hydrogen and carbon dioxide.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Marta Meneghello, Christophe Leger, Vincent Fourmond
Summary: Only two enzymes, CO dehydrogenase and formate dehydrogenase, are capable of directly reducing CO2, producing CO and formate respectively. These metalloenzymes are rapid, energy-efficient, and specific in product. Researchers have studied their mechanisms using protein film electrochemistry, and also exploited their catalytic performance to build biotechnological devices.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Yanxin Gao, Wenjin Li, Xuan Sun, Yingying Zhao, Haishuo Ji, Hang Luo, Geng Wu, Lei Wan, Liyun Zhang
Summary: This study demonstrates the efficient and stable conversion of CO2 into formate using a photocatalytic assembly. The key role of silver nanoclusters in enhancing durability and efficiency is highlighted.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Review
Chemistry, Multidisciplinary
Sarah Bierbaumer, Maren Nattermann, Luca Schulz, Reinhard Zschoche, Tobias J. Erb, Christoph K. Winkler, Matthias Tinzl, Silvia M. Glueck
Summary: Enzymatic carbon dioxide fixation is important for converting inorganic carbon from the atmosphere into organic biomass. However, the thermodynamics and utilization of CO2 make it challenging for biotechnological applications. This review provides an overview of natural CO2-fixing enzymes, discusses their applications, and suggests strategies for improving efficiency. It also explores the potential of using reduced CO2 derivates and offers a techno-economic perspective on reducing CO2 emissions.
Article
Chemistry, Multidisciplinary
Zhichao Jia, Jianan Dang, Guobin Wen, Yanxing Zhang, Zhongwei Chen, Zhengyu Bai, Lin Yang
Summary: Promoting the activity of biological enzymes in vitro holds promise for bioelectrocatalytic reactions, like converting CO2 into valuable chemicals to address CO2 environmental issues. However, this technique remains challenging. In this study, a nanocage structure is synthesized to confine formate dehydrogenase (FDH) in a metal-organic framework, boosting the enzyme's catalytic ability and enhancing its affinity to CO2. The synthesized FDH@ZIF-8 nanocage-structured hybrid (CSH) catalyst shows a 28-fold increase in the conversion rate of CO2 to formic acid compared to using pure FDH. The CSH catalyst traps CO2 and controls its microenvironments, promoting the biological application of enzymes in vitro.
Article
Chemistry, Multidisciplinary
Ryohei Sato, Yutaka Amao
Summary: This study attempted to construct a bienzymatic system consisting of CA and CbFDH for carbon capture, utilization, and storage (CCUS) technology. It was found that the addition of CA in the higher pH region promoted the reduction of CO2 catalyzed by CbFDH to formate.
REACTION CHEMISTRY & ENGINEERING
(2021)
Article
Agricultural Engineering
Mohammad Moniruzzaman, Hung Khac Nguyen, Yu Kiyasu, Takumi Hirose, Yuya Handa, Taro Koide, Seiji Ogo, Ki-Seok Yoon
Summary: This study presents a naturally occurring nanodevice based on bacterial plasma membranes for efficient and selective formate production from H2 and CO2, which has significant potential for various applications.
BIORESOURCE TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Xiu Xing, Yan Liu, Ru-De Lin, Yang Zhang, Zhong-Liu Wu, Xiao-Qi Yu, Kun Li, Na Wang
Summary: In this study, a Zr-based dual-ligand MOFs with pre-installed Rh complex was used for in situ NADH regeneration and immobilization of formic acid dehydrogenase (FDH) to achieve a highly efficient CO2 fixation system. Through detailed investigations, it was shown that the introduction of the photosensitive TCPP ligands increased catalytic active sites and improved photoelectric properties. The electron mediator Rh complex anchored on the MOFs enhanced electron transfer efficiency and facilitated the separation of photogenerated electrons and holes. The optimized photocatalyst, Rh-H2TCPP-UiO-66-NH2, exhibited significantly improved photocatalytic activity for NAD(+) reduction compared to UiO-66-NH2.
Article
Chemistry, Multidisciplinary
Feilong Li, Silvan Scheller, Michael Lienemann
Summary: Metal-dependent formate dehydrogenases (Me-FDHs) are efficient enzymes for CO2 reduction, which can be used for fossil-free production of chemicals using renewable energy. In this study, the role of nucleophilic selenocysteine residue in Escherichia coli formate dehydrogenase H (EcFDH-H) as a ligand of molybdenum atom was investigated. The presence of selenocysteine was found to enhance the efficient CO2 reduction reaction.
JOURNAL OF CO2 UTILIZATION
(2023)
Review
Chemistry, Multidisciplinary
Eleftheria Sapountzaki, Ulrika Rova, Paul Christakopoulos, Io Antonopoulou
Summary: The urgent need to reduce CO2 emissions has driven the development of CO2 capture and utilization technologies. One application is the transformation of CO2 into storage chemicals, such as formic acid, for clean energy carriers. Conventional methods use chemical catalysts for CO2 conversion to formic acid and subsequent H2 release. However, biocatalysts offer a more specific and energy-efficient alternative. Formate dehydrogenase (FDH) catalyzes CO2 conversion to formate, which can be produced by the biocathode in bioelectrochemical systems and electrochemically regenerated. Several microorganisms possessing formate hydrogenlyase or hydrogen-dependent CO2 reductase complexes can catalyze H2 production from formate. Combining these processes enables a CO2-recycling cycle for H2 production, storage, and release with potentially lower environmental impact than conventional methods.
Article
Biotechnology & Applied Microbiology
Mohd Farid Abdul Halim, Leslie A. Day, Kyle C. Costa
Summary: Hydrogenotrophic methanogens produce methane using H-2 as an electron donor, but can also utilize formate or alcohols as alternative electron donors. Methanogens from the order Methanomicrobiales lack genes encoding hydrogenases, but are capable of growth using formate as an electron donor. Studies on Methanoculleus thermophilus highlight the importance of formate as an electron donor for methanogenesis and suggest its close integration into the metabolic pathway of methanogens from the order Methanomicrobiales.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Kwonsu Jung, Woong Kim, Gwon Woo Park, Charles Seo, Ho Nam Chang, Yeu-Chun Kim
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2015)
Article
Biotechnology & Applied Microbiology
Gwon Woo Park, Ilgook Kim, Kwonsu Jung, Charles Seo, Jong-In Han, Ho Nam Chang, Yeu-Chun Kim
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2015)
Article
Biotechnology & Applied Microbiology
Gwon Woo Park, Charles Seo, Kwonsu Jung, Ho Nam Chang, Woong Kim, Yeu-Chun Kim
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2015)
Article
Chemistry, Applied
Yeong Hwan Seo, Gwon Woo Park, Jong-In Han
Article
Chemistry, Physical
Min-Sik Kim, Hana Nur Fitriana, Tae Wan Kim, Sung Gyun Kang, Sang Goo Jeon, Soo Hyun Chung, Gwon Woo Park, Jeong-Geol Na
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2017)
Article
Biochemistry & Molecular Biology
Gwon Woo Park, Ho Nam Chang, Kwonsu Jung, Charles Seo, Yeu-Chun Kim, Jae Hyung Choi, Hee Chul Woo, In-ju Hwang
PROCESS BIOCHEMISTRY
(2017)
Article
Agricultural Engineering
Nulee Jang, Muhammad Yasin, Hyunsoo Kang, Yeubin Lee, Gwon Woo Park, Shinyoung Park, In Seop Chang
BIORESOURCE TECHNOLOGY
(2018)
Review
Agricultural Engineering
Kyoungseon Min, Gwon Woo Park, Young Je Yoo, Jin-Suk Lee
BIORESOURCE TECHNOLOGY
(2019)
Article
Chemistry, Multidisciplinary
Soo Youn Lee, Jiho Min, Sangmin Lee, Hana Nur Fitriana, Min-Sik Kim, Gwon Woo Park, Jin-Suk Lee
APPLIED SCIENCES-BASEL
(2019)
Article
Biotechnology & Applied Microbiology
Min-Sik Kim, Myounghoon Moon, Hana Nur Fitriana, Jin-Suk Lee, Jeong-Geol Na, Gwon Woo Park
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2020)
Article
Agricultural Engineering
Kyoungseon Min, Yong-Soo Park, Gwon Woo Park, Joon-pyo Lee, Myounghoon Moon, Chang Hyun Ko, Jin-Suk Lee
BIORESOURCE TECHNOLOGY
(2020)
Review
Agricultural Engineering
Soo Youn Lee, You-Kwan Oh, Sangmin Lee, Hana Nur Fitriana, Myounghoon Moon, Min-Sik Kim, Jiye Lee, Kyoungseon Min, Gwon Woo Park, Joon-Pyo Lee, Jin-Suk Lee
Summary: This study focuses on microbial CO2 electrobiorefinery based on microbial electrosynthesis, which combines electrochemical and microbial conversion to produce biofuels and higher-value chemicals. Recent developments in bioelectrochemical conversion of CO2 into biofuels and chemicals in MES via microbial CO2-fixation and electricity utilisation reactions are discussed, along with technical approaches to overcome current limitations of MES.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Physical
Gwon Woo Park, Seongsoo Son, Myounghoon Moon, Subin Sin, Kyoungseon Min, Jin-Suk Lee, Ho Nam Chang
Summary: Utilizing volatile fatty acids (VFAs) produced from lipid-extracted yeast (LEY) residue as a carbon source for microbial biodiesel production has the potential to significantly reduce feedstock costs. The optimal conditions for VFA production were determined using the response surface methodology, with the highest concentration achieved at an initial pH of 8.40 and an inoculation of 39.49%.
Article
Engineering, Chemical
Gwon Woo Park, Tae Wan Seo, Hong-Cheol Lee, In-Ju Hwang
KOREAN CHEMICAL ENGINEERING RESEARCH
(2016)
