期刊
FEBS OPEN BIO
卷 11, 期 2, 页码 482-493出版社
WILEY
DOI: 10.1002/2211-5463.13075
关键词
exosome; extracellular vesicles; heart; liver; muscle; tissue; ultracentrifugation
资金
- Life Science Institute, Inc.,
- Mitsubishi Chemical Holdings Corporation
Extracellular vesicles (EVs), as nanosized vesicles released by cells as intracellular messengers, have high potential as biomarkers. A comparison of methods for separating EVs from solid tissues showed that an ultracentrifugation-based method yielded higher proportion of EVs positive for EV-related markers compared to precipitation method, with some tissue-specific modifications improving the yield and purity of collected EVs.
Extracellular vesicles (EVs), which are nanosized vesicles released by cells as intracellular messengers, have high potential as biomarkers. EVs are usually collected from in vitro sources, such as cell culture media or biofluids, and not from tissues. Techniques enabling direct collection of EVs from tissues will extend the applications of EVs. We compared methods for separating EVs from solid liver, heart, and skeletal muscle. Compared with a precipitation method, an ultracentrifugation-based method for collection of EVs from solid tissues yielded a higher proportion of EVs positive for EV-related markers, with minimum levels of intracellular organelle-related markers. Some tissue-specific modifications, such as a sucrose cushion step, may improve the yield and purity of the collected EVs.
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