Article
Biochemistry & Molecular Biology
Kai Shi, Yingling Lu, Xiaolu Chen, Dongfeng Li, Wenxing Du, Minli Yu
Summary: Tet2 plays a crucial role in chicken myogenesis by regulating the demethylation of genomic DNA and histones to control the expression of MRFs, thus contributing to myoblast differentiation. The expression of Tet2 is significantly elevated during myoblast differentiation, leading to an increase in 5hmC levels and a decrease in H3K9me2 and H3K27me3 levels. Knockdown of Tet2 inhibits the formation of multinucleated myotubes and reduces pivotal MRFs expression.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yinglin Lu, Kai Shi, Haobin Wang, Heng Cao, Fan Li, Jing Zhou, Minli Yu, Debing Yu
Summary: Ascorbic acid and 5-AZA promote myogenic differentiation by regulating DNA demethylation and histone modification levels, with Tet2 playing a key role in this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Jun Liu, Suji Min, Dongchan Kim, Jihyun Park, Eunchae Park, Shanshan Pei, Youngil Koh, Dong-Yeop Shin, Ja Min Byun, Myunggon Ko, Sung-Soo Yoon, Junshik Hong
Summary: Vitamin C is known to regulate hematopoietic stem cells and leukemogenesis. However, glucose transporter 3 (GLUT3) deficiency in acute myeloid leukemia (AML) blocks the uptake of vitamin C and diminishes its clinical benefits. This study aimed to investigate the therapeutic potential of restoring GLUT3 in AML. Restoring GLUT3 expression in AML cells enhanced TET2 activity and improved the anti-leukemic effect of vitamin C treatments. Pharmacological salvage of GLUT3 deficiency has the potential to improve the effectiveness of vitamin C in AML treatment.
Article
Immunology
Zheng-jun Gao, Wen-ping Li, Xin-tao Mao, Tao Huang, Hao-li Wang, Yi-ning Li, Bao-qin Liu, Jiang-yan Zhong, Chai Renjie, Jin Jin, Yi-yuan Li
Summary: This study reveals that deficiency of Uhrf1 in myeloid cells significantly upregulates Ifnb expression, enhancing resistance to viral infection. Whole-genome bisulfite sequencing identified a single nucleotide methylation site in the Ifnb promoter region that disrupts IRF3 recruitment. Further experiments confirmed the critical role of this methylated site in regulating Ifnb expression and antiviral responses.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Review
Oncology
Lina Hu, Xuanye Zhang, Huifeng Li, Suxia Lin, Shengbing Zang
Summary: Recent advances in exome sequencing and comprehensive genomic studies have provided new insights into the pathogenesis and mutational landscape of Angioimmunoblastic T-cell lymphoma (AITL). The discovery of recurrent mutations in TET2, DNMT3A, IDH2, and RHOA has highlighted their essential role in AITL. Furthermore, the detection of TET2 mutations in bystander B cells in AITL samples has advanced our understanding of the disease's mechanisms and its association with B-cell lymphoma.
Correction
Multidisciplinary Sciences
Kenichi Kawahori, Yoshitaka Kondo, Xunmei Yuan, Yuki Kawasaki, Nozomi Hanzawa, Kazutaka Tsujimoto, Fumiko Wada, Takashi Kohda, Akihito Ishigami, Tetsuya Yamada, Yoshihiro Ogawa, Koshi Hashimoto
Summary: The paper has been amended and the updated version can be accessed through a link at the top of the original paper.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Feng Wan, Yue-Wen Tang, Xuan-Li Tang, Ya-Yu Li, Ru-Chun Yang
Summary: The study revealed that triptolide can reverse podocyte EMT process and increase TET2 expression, protecting podocytes from injury and reducing proteinuria. Furthermore, triptolide may exert its protective effect on podocytes by regulating DNA demethylation, particularly at the promoters of NEPH1 and nephrin.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2021)
Review
Cell Biology
Boyi Cong, Qian Zhang, Xuetao Cao
Summary: TET2, a crucial regulator in hematopoiesis, is involved in the development of hematological malignancies. Recent studies have shown its important role in innate immune homeostasis and inflammation resolution. Understanding the functions and regulation of TET2 can provide insights for intervention in pathological processes caused by TET2 dysregulation.
Article
Oncology
Yixi Su, Qiang Huang, Li Lu, Hu Qu, Dejuan Wang, Jianguang Qiu, Weiqian Li, Mengmeng Lin, Huanliang Liu, Zhongyang Wang, Xiangling Yang
Summary: NPTX2 expression is significantly reduced in prostate cancer and its promoter is highly methylated. NPTX2 acts as a tumor suppressor gene by inhibiting cell proliferation in prostate cancer.
Article
Multidisciplinary Sciences
Karina Mendes, Sandra Schmidhofer, Julia Minderjahn, Dagmar Glatz, Claudia Kiesewetter, Johanna Raithel, Julia Wimmer, Claudia Gebhard, Michael Rehli
Summary: The study examines changes in DNA methylation during the differentiation of post-mitotic human monocytes (MO), revealing that EGR2 interacts with TET2 and is required for DNA demethylation at its binding sites, identifying EGR2 as an epigenetic pioneer factor in human MO.
NATURE COMMUNICATIONS
(2021)
Review
Neurosciences
Lin Qin, Chong Qiao, Volney Sheen, Yu Wang, Jie Lu
Summary: The overexpression of DNMT3L in DS mainly affects global RNA expression of genes rather than DNA methylation, leading to the activation of transcription factors such as HES3, ASCL1, NEUROG2, and CDKN1A, which promotes cell cycle exit and neural differentiation. This phenomenon is independent of DNA methylation and may contribute to the abnormal phenotypes observed in Down syndrome cortex.
PROGRESS IN NEUROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ding Peng, Anbang He, Shiming He, Guangzhe Ge, Shuo Wang, Weimin Ci, Xuesong Li, Dan Xia, Liqun Zhou
Summary: This study explores the role of PD-L1 in renal cancer and reveals the effects of ascorbic acid supplementation and TET2 depletion on anti-PD-L1 therapy. The results suggest that ascorbic acid enhances immunotherapy efficacy, while loss of TET2 impairs anti-tumor immunity. The study also identifies a new strategy of stimulating TET activity to improve immunotherapy for renal cell carcinoma.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Medicine, Research & Experimental
Yuhang Jiang, Fengfeng Li, Bowen Gao, Mengjun Ma, Meng Chen, Yanfeng Wu, Weidong Zhang, Yangbai Sun, Sanhong Liu, Huiyong Shen
Summary: The study identified KDM6B as significantly upregulated in osteosarcoma patients, and high expression was associated with poor prognosis. Targeting KDM6B inhibited cell metastasis and further investigations revealed KDM6B promotes osteosarcoma metastasis by modulating LDHA expression.
Article
Multidisciplinary Sciences
Andrew Baessler, Camille L. Novis, Zuolian Shen, Jelena Perovanovic, Mark Wadsworth, Kendall A. Thiede, Linda M. Sircy, Malia Harrison-Chau, Nguyen X. Nguyen, Katherine E. Varley, Dean Tantin, J. Scott Hale
Summary: Following viral infection, Tet2-deficient CD4(+) T cells exhibit a preference for differentiating into highly functional germinal center T follicular helper (T-FH) cells. Tet2 coordinates with multiple transcription factors to regulate the demethylation and expression of target genes, including those that inhibit T-FH differentiation.
Article
Biochemistry & Molecular Biology
Ekaterina Romanova, Anatoliy Zubritskiy, Anna Lioznova, Adewale J. Ogunleye, Vasily A. Golotin, Anna A. Guts, Andreas Lennartsson, Oleg N. Demidov, Yulia A. Medvedeva
Summary: Acute myeloid leukemia (AML) is a rapidly progressing disease with a high mortality rate. Mutations in transcription factors RUNX1 and CEBPA in AML patients can affect the methylation of regulatory sites, leading to gene silencing, most likely in a TET2-dependent manner. Demethylation therapy can restore gene expression and increase sensitivity to chemotherapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Yunqing Ma, Jiayuan Zhang, Weijie Yin, Zhenchao Zhang, Yan Song, Xing Chang
Article
Biochemistry & Molecular Biology
Yuanzheng Peng, Juanjuan Yuan, Zhenchao Zhang, Xing Chang
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Yanhong Li, Jiayuan Zhang, Di Zhang, Xiaowu Hong, Yu Tao, Songcun Wang, Yuanyuan Xu, Hailan Piao, Weijie Yin, Min Yu, Yin Zhang, Qiang Fu, Dajin Li, Xing Chang, Meirong Du
Article
Biochemistry & Molecular Biology
Yanhong Li, Jiayuan Zhang, Di Zhang, Xiaowu Hong, Yu Tao, Songcun Wang, Yuanyuan Xu, Hailan Piao, Weijie Yin, Min Yu, Yin Zhang, Qiang Fu, Dajin Li, Xing Chang, Meirong Du
Article
Immunology
Zhizhang Wang, Weijie Yin, Lizhen Zhu, Jia Li, Yikun Yao, Feifei Chen, Mengmeng Sun, Jiayuan Zhang, Nan Shen, Yan Song, Xing Chang
Article
Biochemistry & Molecular Biology
Weijie Yin, Yan Song, Xing Chang
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Juanjuan Yuan, Yunqing Ma, Tao Huang, Yanhao Chen, Yuanzheng Peng, Bing Li, Jia Li, Yuchen Zhang, Bing Song, Xiaofang Sun, Qiurong Ding, Yan Song, Xing Chang
Article
Cardiac & Cardiovascular Systems
Jia Li, Kaiying Wang, Yuchen Zhang, Tuan Qi, Juanjuan Yuan, Lei Zhang, Han Qiu, Jinxi Wang, Huang-Tian Yang, Yi Dai, Yan Song, Xing Chang
Summary: The study analyzed a novel murine model of DMD and examined the feasibility of using a cytidine base editor for exon skipping in vivo. The results showed that AAV9-eTAM treatment in the mouse model restored dystrophin and improved cardiac and skeletal muscle functions significantly.
Letter
Cardiac & Cardiovascular Systems
Jia Li, Xing Chang
Article
Cell Biology
Lei-Lei Chen, Carmen Morcelle, Zhou-Li Cheng, Xiufei Chen, Yanping Xu, Yajing Gao, Jun-Bin Song, Zhijun Li, Matthew Smith, Miao Shi, Yezhang Zhu, Neng Zhou, Meng Cheng, Chenxi He, Kwei-Yan Liu, Guoping Lu, Lei Zhang, Cheng Zhang, Jinye Zhang, Yiping Sun, Tuan Qi, Yingying Lyu, Zhi-Zhong Ren, Xian-Ming Tan, Fei Lan, Ying Liu, Hui Yang, Caiwen Duan, Xing Chang, Yufeng Zhou, Li Shen, Albert Baldwin, Kun-Liang Guan, Yue Xiong, Dan Ye, Jiayong Yin, Maoxiang Qian
Summary: This study reveals that ITA acts as an immune modulatory metabolite by inhibiting TET enzymes to suppress inflammatory responses. ITA binds to the catalytic site of TET2, inhibiting its regulation of lipopolysaccharide-induced genes and reducing acute pulmonary edema and organ injury caused by lipopolysaccharide stimulation.
NATURE CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Lizhen Zhu, Geng Li, Zhixin Liang, Tuan Qi, Kui Deng, Jiancheng Yu, Yue Peng, Jusheng Zheng, Yan Song, Xing Chang
Summary: Iron deficiencies are the most common nonenteric syndromes observed in patients with inflammatory bowel disease. This study reveals that the homeostasis of regulatory T cells in the intestine depends on high cellular iron levels, which are facilitated by the short-chain fatty acid pentanoate produced by intestinal microbiota. The findings demonstrate the association between nutrient uptake, specifically iron, and immune tolerance in the intestine.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Han Qiu, Geng Li, Juanjuan Yuan, Dian Yang, Yunqing Ma, Feng Wang, Yi Dai, Xing Chang
Summary: This study demonstrates that base editors can efficiently induce exon skipping by disrupting exonic splicing enhancers, providing a new treatment strategy for DMD and other RNA mis-splicing diseases and expanding the repertoire of potential targets.
Article
Cell Biology
Boyi Zhang, Qilai Long, Shanshan Wu, Qixia Xu, Shuling Song, Liu Han, Min Qian, Xiaohui Ren, Hanxin Liu, Jing Jiang, Jianming Guo, Xiaoling Zhang, Xing Chang, Qiang Fu, Eric W-F Lam, Judith Campisi, James L. Kirkland, Yu Sun
Summary: Cellular senescence is regulated by histone H3-specific demethylase KDM4, which is associated with changes in H3K9/H3K36 methylation and poorer survival in prostate cancer patients. Targeting KDM4 reduces senescence-associated secretory phenotype (SASP) in stromal cells, promoting cancer cell apoptosis and prolonging survival in experimental animals. This study suggests KDM4 as a key modulator in manipulating cellular senescence and limiting its contribution to age-related pathologies, including cancer.
Article
Biochemical Research Methods
Yunqing Ma, Juanjuan Yuan, Xing Chang