Article
Plant Sciences
Prassan Choudhary, Sanjay Kumar Goswami, Hillol Chakdar, Shaloo Verma, Shobit Thapa, Alok Kumar Srivastava, Anil Kumar Saxena
Summary: A LAMP assay was developed to accurately and quickly detect the pathogen Sarocladium oryzae, the causal agent of sheath rot in rice, from live-infected tissues, seeds, weeds, and environmental samples. The assay demonstrated high sensitivity, detecting the pathogen in weed species and rice varieties. It can effectively be used for detection and screening of S. oryzae in live samples including seeds and field soil.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
R. Logeshwari, C. Gopalakrishnan, A. Kamalakannan, J. Ramalingam, R. Saraswathi
Summary: A rapid and highly specific detection method based on LAMP technology was developed in this study for the seed-borne pathogen Sarocladium oryzae. This method offers quick and reliable detection of the pathogen, which is important for maintaining the health of rice seeds.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Junning Ma, Jean-Benoit Morel, Michael Riemann, Peter Nick
Summary: This study dissects the JA-dependent defense signaling in rice for both compatible and incompatible interactions by using JA-deficient mutants cpm2 and hebiba. The results demonstrate the pivotal role of JA in the basal immunity of rice in its resistance to the Rice Blast Fungus in both types of interactions.
Article
Biotechnology & Applied Microbiology
Yanping Tan, Xiaolin Yang, Minghao Pei, Xin Xu, Chuntai Wang, Xinqiong Liu
Summary: The study aimed to investigate the regulation of gene expression in the interaction between rice and Magnaporthe oryzae using gene chip technology. Changes in gene expression at different time points post-inoculation were analyzed, revealing significant differentially expressed genes and identifying new genes related to rice blast. This research may contribute to the discovery of novel resistance genes and improve the understanding of rice blast development mechanisms.
Article
Plant Sciences
Guihua Duan, Chunqin Li, Yanfang Liu, Xiaoqing Ma, Qiong Luo, Jing Yang
Summary: This study utilized UHPLC/Q-TOF-MS to identify altered metabolites in transgenic rice infected with M. oryzae, revealing organic acids, organic oxygen compounds, lipids, and lipid-like molecules. The research showed that carbohydrate and amino acid metabolic pathways were involved in plant defense responses and resistance strengthening. The study also found that the identified metabolites could be utilized as an optimal marker for M.oryzae defense in rice.
Article
Plant Sciences
Huajun Zhu, Hu Zhou, Zuohua Ren, Erming Liu
Summary: Bacillus subtilis JN005 shows growth promotion and biocontrol efficacy against rice blast, improving germination and growth of rice seeds, enhancing defense enzyme activities in rice leaves, and reducing disease incidence by 79% and 76% in curative and preventive treatments, respectively. Additionally, field experiments demonstrate that spraying with JN005 suspension at 1x10(7) cfu/mL can effectively control rice blast and increase rice production yield.
JOURNAL OF PLANT GROWTH REGULATION
(2022)
Article
Biochemistry & Molecular Biology
Shang Gao, Yan Hou, Qiwei Huang, Pengzhi Wu, Zhikai Han, Danhong Wei, Huabin Xie, Fengwei Gu, Chun Chen, Jiafeng Wang
Summary: In this study, the important role of Osa-miR11117 in regulating rice defense mechanisms was investigated. It was found that Osa-miR11117 targets OsPAO4 to regulate blast resistance by adjusting polyamine metabolism and ethylene signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Jessie Fernandez, Victor Lopez, Lisa Kinch, Mariel A. Pfeifer, Hillery Gray, Nalleli Garcia, Nick Grishin, Chang-Hyun Khang, Kim Orth
Summary: The study identified two metalloproteinase enzymes, MoMca1 and MoMca2, in M. oryzae, which play significant roles in fungal growth and development, potentially regulating stress responses. The absence of these enzymes resulted in delays in fungal morphogenesis and insights into the mechanisms of M. oryzae pathogenicity.
Article
Agronomy
Dagang Tian, Zaijie Chen, Yan Lin, Tingmin Liang, Ziqiang Chen, Xinrui Guo, Feng Wang, Zonghua Wang
Summary: This study investigates the diversity, composition, and assembly of microbial communities associated with the rhizosphere soils and endosphere of Piz-t-KJ201 and NPB-KJ201. The host genotype and inoculation pattern of M.oryzae played dominant roles in determining the microbial community assemblage. Metabolomics analysis revealed that some metabolites may influence changes in bacterial communities.
Article
Agronomy
Na Cui, Xiaofeng Chen, Yan Shi, Meirong Chi, Jiantuan Hu, Kunlong Lai, Zonghua Wang, Haifeng Wang
Summary: DNA methylation plays a role in regulating the expression of coding and non-coding regions in plants. A study using whole-genome bisulfite sequencing revealed temporal DNA methylation changes in rice after infection with Magnaporthe oryzae, showing a slight increase in global DNA methylation levels compared to Arabidopsis. Over 38,000 differentially methylated regions (DMRs) were identified, with hypermethylated DMRs dominating over hypomethylated DMRs, most of which were located in transposable element regions. Transcription of NBS-LRR family genes was affected by changes in DNA methylation, indicating the essential role of DNA methylation in rice response to M. oryzae infection.
Article
Microbiology
Pengyun Huang, Huijuan Cao, Yan Li, Siyi Zhu, Jing Wang, Qing Wang, Xiaohong Liu, Fu-Cheng Lin, Jianping Lu
Summary: This study reveals the functional diversity and complexity of melanin in different strains of Magnaporthe oryzae. Melanin promotes spore production in the rice blast pathogen, but its effect is related to the background melanin content of the wild-type strains. Transcription factors like Cnf1 have opposite effects on conidiophore differentiation in different strains. Pig1 regulates melanin synthesis in hyphae but not in conidiophores, spores, or appressoria.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
K. J. Peeters, K. Audenaert, M. Hofte
Summary: The fungus Sarocladium oryzae causes rice sheath rot and produces two phytotoxins, cerulenin and helvolic acid. The production of helvolic acid correlates with the virulence of the fungus. Different toxin-producing isolates of S. oryzae affect the diversity and composition of rice endophytes, with less virulent isolates increasing diversity and virulent isolates causing shifts in species composition. The endophyte community of healthy rice plants is dominated by Bacillus cereus, which is enriched in lesions produced by low-virulent S. oryzae isolates.
FEMS MICROBIOLOGY ECOLOGY
(2021)
Article
Plant Sciences
Vincent Ninkuu, Jianpei Yan, Lin Zhang, Zhenchao Fu, Tengfeng Yang, Shupeng Li, Beibei Li, Jiaqi Duan, Jie Ren, Guangyue Li, Xiufen Yang, Hongmei Zeng
Summary: This study investigated the mechanism of Hrip1-induced immunity in rice using transcriptome and metabolomic techniques. The results showed that Hrip1 activates key genes and molecular switches to enhance resistance against pathogens and increases the production of lignin and phytoalexins, thereby strengthening rice immunity.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Jianglin Zhang, Zhifeng Lu, Rihuan Cong, Tao Ren, Jianwei Lu, Xiaokun Li
Summary: This study examined the effects of Sarocladium oryzae infection on the endophytic community and nutrient uptake process of rice plants. The results showed that S. oryzae infection altered the endophytic community, inhibited nutrient uptake, and decreased the level of jasmonic acid, which is essential for potassium uptake. These findings highlight the importance of potassium in resistance to S. oryzae infection by modulating endophyte community diversity and enhancing the nutrient-uptake capacity of the host plant.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Microbiology
Basavantraya N. Devanna, Priyanka Jain, Amolkumar U. Solanke, Alok Das, Shallu Thakur, Pankaj K. Singh, Mandeep Kumari, Himanshu Dubey, Rajdeep Jaswal, Deepak Pawar, Ritu Kapoor, Jyoti Singh, Kirti Arora, Banita Kumari Saklani, Chandrappa AnilKumar, Sheshu Madhav Maganti, Humira Sonah, Rupesh Deshmukh, Rajeev Rathour, Tilak Raj Sharma
Summary: This review discusses the importance of rice and blast disease in the global context, as well as the molecular interactions between the blast pathogen and rice. The article provides detailed insights into the genomics and molecular biology of the blast pathogen, as well as the application of resistance QTLs and R genes.
Article
Microbiology
Raman Thangavelu, Esack Edwinraj, Muthukathan Gopi, Periyasamy Pushpakanth, Kotteswaran Sharmila, Manivasakan Prabaharan, Murugan Loganathan, Subbaraya Uma
Summary: This study identified the genomic differences and effector gene profiles of Indian Foc isolates, and developed specific molecular markers for Indian Foc R1, R4, and TR4. These markers can be used for accurate diagnosis and detection of the pathogen.
Article
Multidisciplinary Sciences
Sahana N. Banakar, M. K. PrasannaKumar, H. B. Mahesh, P. Buela Parivallal, M. E. Puneeth, Chirag Gautam, D. Pramesh, T. N. Shiva Kumara, T. R. Girish, Sailaja Nori, Shrikumar Surya Narayan
Summary: Red seaweed-derived biostimulants promote plant health and protect against abiotic stress conditions. This study investigates the efficacy of red seaweed biostimulants on rice under fungicidal stress and describes the underlying mechanism.
SCIENTIFIC REPORTS
(2022)
Article
Genetics & Heredity
Talambedu Usha, Sushil Kumar Middha, Dinesh Babu, Arvind Kumar Goyal, Anupam J. Das, Deepti Saini, Aditya Sarangi, Venkatesh Krishnamurthy, Mothukapalli Krishnareddy Prasannakumar, Deepak Kumar Saini, Kora Rudraiah Sidhalinghamurthy
Summary: This study presents a high-quality de novo draft genome assembly of the pomegranate cultivar Bhagwa, providing insights into its genomic features and pharmacological properties. The genome assembly reveals the abundance of genes involved in the biosynthesis of medicinal compounds and annotations predict their putative functions. This information can facilitate the breeding of new pomegranate varieties with improved yield and quality.
FRONTIERS IN GENETICS
(2022)
Article
Plant Sciences
Garima Pal, Rahul Bakade, Sanjay Deshpande, V Sureshkumar, Swathi S. Patil, Akashata Dawane, Subham Agarwal, Vidya Niranjan, M. K. PrasannaKumar, Ramu S. Vemanna
Summary: The study focused on the effects of drought stress, Xanthomonas oryzae pv. oryzae (Xoo) pathogen infection, and combined stress on different rice genotypes. By conducting RNA sequencing, numerous important genes have been identified from resistant genotypes, which could be used as molecular markers and targets for developing durable resistant rice cultivars.
Review
Biochemistry & Molecular Biology
Roopashree Byregowda, Siddegowda Rajendra Prasad, Ralf Oelmueller, Karaba N. Nataraja, M. K. Prasanna Kumar
Summary: This review describes the relationship between plants and endophytic fungi and how the latter promote plant growth, productivity, and drought tolerance. The application of endophytic fungi offers a lower-cost and more efficient alternative to conventional breeding and biotechnological approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Adke Raghunandana, Devanna Pramesh, Sunkad Gururaj, Chittaragi Amoghavarsha, Manoj K. Yadav, Umakanta Ngangkham, Hanumanthappa Deeshappa Pushpa, M. K. Prasannakumar, B. T. Raghavendra, Naik R. Harischandra, Siddepalli E. Manjunatha, S. T. Yenjerappa
Summary: This study analyzed the virulence, genetic diversity, and population structure of Xanthomonas oryzae pv. oryzae (Xoo) isolates collected from different rice ecosystems in Karnataka, India. Nine pathotype groups were identified, with KPthX-6 being highly virulent and KPthx-2 being less virulent. Resistance genes Xa4, xa5, xa13, and Xa21 were effective against all pathotypes. The isolates were categorized into three clusters and five subgroups based on genetic diversity. The study provides valuable information for the eco-specific management of bacterial leaf blight (BLB) of rice.
PLANT PROTECTION SCIENCE
(2023)
Article
Agronomy
Sharanabasav Huded, Devanna Pramesh, Amoghavarsha Chittaragi, Shankarappa Sridhara, Eranna Chidanandappa, Muthukapalli K. Prasannakumar, Channappa Manjunatha, Balanagouda Patil, Sandip Shil, Hanumanthappa Deeshappa Pushpa, Adke Raghunandana, Indrajeet Usha, Siva K. Balasundram, Redmond R. Shamshiri
Summary: This study investigated the spatial distribution of False smut disease (FSD) in rice cultivation in South India. It identified clusters of FSD in coastal and interior districts of Karnataka and Tamil Nadu, providing important information for the development of management strategies.
Article
Plant Sciences
M. Chaithra, N. S. Pankaja, P. S. Benherlal, H. B. Mahesh
Summary: Twenty-one Trichoderma isolates were collected from different cropping locations in Karnataka, and their morphological and molecular characteristics were analyzed. The study showed that these isolates exhibited strong antagonistic activity against foliar pathogens.
ARCHIVES OF PHYTOPATHOLOGY AND PLANT PROTECTION
(2022)
Article
Genetics & Heredity
Sahana N. Banakar, M. K. Prasannakumar, P. Buela Parivallal, D. Pramesh, H. B. Mahesh, Aditya N. Sarangi, M. E. Puneeth, Swathi S. Patil
Summary: Red seaweed extracts have been found to enhance the resistance of several crops to biotic stress. This study focused on the transcriptional modifications in rice plants treated with seaweed biostimulant to understand their specific response to blast disease. The results showed that seaweed priming induced the defense response in rice against blast disease through the accumulation of reactive oxygen species, activation of defense-related genes, and reinforcement of the cell wall. The findings suggest that seaweed bio-stimulants can be used as a potential strategy to protect rice crops from blast disease.
FRONTIERS IN GENETICS
(2023)
Article
Microbiology
N. Kavya, M. K. Prasannakumar, Gopal Venkateshbabu, Vidya Niranjan, Akshay Uttarkar, P. Buela Parivallal, Sahana N. Banakar, H. B. Mahesh, Pramesh Devanna, K. G. Manasa, Tagginahalli N. Shivakumara
Summary: In this study, seven novel effectors in Rhizoctonia solani were identified and their functions, reactivity, and stability were evaluated. Target proteins involved in the regulation of rice defense mechanisms were also identified. These findings have implications for the management of sheath blight in rice.
Article
Entomology
C. Manjunatha, V. Velavan, R. Rangeshwaran, M. Mohan, A. Kandan, G. Sivakumar, A. N. Shylesha, M. K. Prasanna Kumar, D. Pramesh, M. Sujithra, H. K. Ranganath, S. N. Sushil
Summary: The study showed that the combination of Bacillus thuringiensis with Metarhizium robertsii and M. majus is effective in managing the rhinoceros beetle in coconut orchards. The soil drench method is more effective than the topical spray method in controlling the beetles' larval population. The study also confirmed that B. thuringiensis, M. robertsii, and M. majus are compatible and have a synergistic effect.
EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL
(2023)
Article
Biotechnology & Applied Microbiology
B. S. Chandrashekar, M. K. Prasannakumar, Gopal Venkateshbabu, H. B. Mahesh, M. E. Puneeth, Karthik S. Narayan, P. Buela Parivallal, D. Pramesh, Sahana N. Banakar, Swati S. Patil
Summary: Bacterial soft rot in carrots is a devastating disease, and in this study, eight isolates of the bacterial pathogen causing soft rot in carrots were collected from India. The pathogen was identified as Klebsiella variicola through 16S rRNA sequencing, and its morphological characteristics were investigated under scanning electron microscopy. Two novel strains of Bacillus velezensis (A6 and P42) were found to have strong antagonistic activity against K. variicola, producing bioactive compounds with antibacterial and antifungal properties. These strains can be used as biocontrol agents to manage soft rot diseases of carrot under field conditions.
LETTERS IN APPLIED MICROBIOLOGY
(2023)
Article
Engineering, Multidisciplinary
S. M. Usha, H. B. Mahesh
Summary: This study presents a method to remotely monitor and efficiently water agricultural fields to increase crop production. Advanced technologies such as image processing and neural networks were employed to study the plants' conditions and improve the quality of the image.
ENGINEERING TECHNOLOGY & APPLIED SCIENCE RESEARCH
(2022)