Article
Biochemical Research Methods
Joana Pinto, Filipa Amaro, Ana Rita Lima, Carina Carvalho-Maia, Carmen Jeronimo, Rui Henrique, Maria de Lourdes Bastos, Marcia Carvalho, Paula Guedes de Pinho
Summary: The study utilized urinary volatilome profiling to identify a volatile signature for distinguishing ccRCC patients from controls. Analysis revealed significant differences in 22 volatile metabolites between the two groups, with a six-biomarker panel showing high sensitivity and specificity for ccRCC detection. These findings suggest potential for the development of a bioelectronic sensor based on the dysregulated energy metabolism and enzyme overexpression in ccRCC urinary volatilome signature.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Zuhu Yu, Chong Lu, Bin Lu, Hong Gao, Rongfang Liang, Wuxing Xiang
Summary: This study identified necroptosis-related microRNAs (miRNAs) associated with the prognosis of clear cell renal cell carcinoma (ccRCC), and developed a prognostic signature to predict overall survival in ccRCC patients. The targeted genes of these miRNAs were predicted using miRNA databases, and their functional and pathway associations with ccRCC prognosis were investigated. The differential expression of the three miRNAs in the signature was validated in ccRCC tissues.
Article
Medicine, General & Internal
Xing He, Feng Tian, Fei Guo, Fangxing Zhang, Huiyong Zhang, Jin Ji, Lin Zhao, Jingyi He, Yutian Xiao, Longman Li, Chunmeng Wei, Caihong Huang, Yexin Li, Feng Zhang, Bo Yang, Huamao Ye, Fubo Wang
Summary: This study identified novel potential biomarkers for clear cell renal cell carcinoma (ccRCC) based on exosomal mRNA profiling. Two emRNA-based signatures were developed for the early detection of ccRCC and differential diagnosis of uncertain renal masses. These signatures showed high accuracy in distinguishing ccRCCs from healthy controls and benign renal masses, indicating their potential as promising and noninvasive biomarkers for ccRCC detection.
Article
Radiology, Nuclear Medicine & Medical Imaging
Fatma Eldihimi, Cynthia Walsh, Rebecca M. Hibbert, Khalid Al Nasibi, Jana Sheinis Pickovsky, Nicola Schieda
Summary: A CT scoring system can be used to diagnose ccRCC among small solid renal masses and guide renal mass biopsy decisions in clinical practice.
EUROPEAN RADIOLOGY
(2023)
Article
Acoustics
Lin Li, Hanbo Wu, Shuai Hu, Yanfei Yu, Zhicong Chen, Pu Wang, Liqun Zhou, Rui Li, Lin Yao, Shuhua Yue
Summary: The integration of photoacoustic tomography (PAT) with ultrasound imaging can differentiate normal renal tissues and clear cell renal cell carcinoma (ccRCC) tissues accurately and with high sensitivity and specificity.
Review
Endocrinology & Metabolism
Haiyan Zhu, Xin Wang, Shihao Lu, Kongbo Ou
Summary: Clear cell renal cell carcinoma (ccRCC) is a malignancy characterized by metabolic reprogramming, which provides potential targets for therapeutic interventions. This review summarizes recent discoveries of metabolic alterations in ccRCC, including changes in glucose, lipid, and amino acid metabolism. It also discusses the development of metabolic drugs targeting these pathways and proposes future trends in drug development. Overall, this review highlights the potential for developing new treatments for ccRCC based on metabolic alterations.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Sumeyye Su, Shaya Akbarinejad, Leili Shahriyari
Summary: This study utilized digital cytometry to estimate the percentage of different immune cell types in 526 renal tumors, revealing that the most common immune cell types in clear cell renal cell carcinomas (ccRCC) tumors are CD8+ T-cells, macrophages, and CD4+ T-cells. Furthermore, unsupervised clustering analysis identified four distinct groups of ccRCC tumors based on the relative numbers of immune cells.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Jacek Rysz, Beata Franczyk, Janusz Lawinski, Anna Gluba-Brzozka
Summary: Renal cell carcinoma is a group of malignant tumors of the renal cortex. Clear cell papillary renal cell carcinoma, a specific subtype, shares morphologic and genetic features with other subtypes but also has distinct clinical behavior. Differentiating clear cell papillary renal cell carcinoma from other subtypes is crucial for appropriate management.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Jose Pedro Sequeira, Vera Constancio, Sofia Salta, Joao Lobo, Daniela Barros-Silva, Carina Carvalho-Maia, Jessica Rodrigues, Isaac Braga, Rui Henrique, Carmen Jeronimo
Summary: Early detection of renal cell carcinoma (RCC) is crucial, and using circulating microRNAs for early, minimally invasive detection and diagnosis is feasible.
Article
Oncology
Wei Zhang, Wenqiang Liu, Yiren Yang, Chengwu Xiao, Yutian Xiao, Xiaojie Tan, Qingyang Pang, Han Wu, Meimian Hua, Xiaolei Shi
Summary: By integrating transcriptomic landscape and urinary signature, a urine-based prognostic classifier consisting of VSIG4, TGFBI, and P4HB was developed to predict ccRCC prognosis and may facilitate ccRCC molecular subtyping and treatment.
FRONTIERS IN ONCOLOGY
(2023)
Article
Genetics & Heredity
Enyang Zhao, Xuedong Li, Bosen You, Jinpeng Wang, Wenbin Hou, Qiong Wu
Summary: The study aims to identify kidney renal clear cell carcinoma using miRNAs as biomarkers. By employing a feature selection strategy and Gaussian mixture model, five miRNAs were identified as a signature for diagnosis.
FRONTIERS IN GENETICS
(2022)
Article
Oncology
Madonna R. Peter, Fang Zhao, Renu Jeyapala, Shivani Kamdar, Wei Xu, Cynthia Hawkins, Andrew J. Evans, Neil E. Fleshner, Antonio Finelli, Bharati Bapat
Summary: This study analyzed the expression of various circRNAs in urinary sediment samples and found significantly reduced levels of circEGLN3 and circSOD2 in urine from ccRCC patients compared to healthy controls. The findings highlight the potential of circRNA markers as non-invasive diagnostic tools for detecting malignant RCC.
FRONTIERS IN ONCOLOGY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Khalid Al Nasibi, Jana Sheinis Pickovsky, Fatma Eldehimi, Trevor A. Flood, Luke T. Lavallee, Anne K. Tsampalieros, Nicola Schieda
Summary: This study developed a CT-based algorithm for diagnosing ccRCC among small solid renal masses. By evaluating the mass-to-cortex corticomedullary attenuation ratio and heterogeneity score, the algorithm can accurately diagnose ccRCC.
AMERICAN JOURNAL OF ROENTGENOLOGY
(2022)
Article
Genetics & Heredity
You Zuo, Shuai Fu, Zhongwei Zhao, Zeyan Li, Yijian Wu, Tienan Qi, Jianguo Zheng, Qinglong Du, Zhonghua Xu, Nengwang Yu
Summary: This study constructed a sarcomatoid-associated gene risk index (SAGRI) to predict the prognosis of renal cell carcinoma and found that SAGRI has a high predictive power. The study also suggested that the SAGRI-HIGH subgroup may benefit from immunotherapy.
FRONTIERS IN GENETICS
(2022)
Article
Medicine, General & Internal
Jesus Miranda-Poma, Lucia Trilla-Fuertes, Rocio Lopez-Vacas, Elena Lopez-Camacho, Eugenia Garcia-Fernandez, Ana Pertejo, Maria Lumbreras-Herrera, Andrea Zapater-Moros, Mariana Diaz-Almiron, Antje Dittmann, Juan Angel Fresno Vara, Enrique Espinosa, Pilar Gonzalez-Peramato, Alvaro Pinto-Marin, Angelo Gamez-Pozo
Summary: This study aimed to explore the tumor proteome of patients diagnosed with localized clear cell renal cancer (ccRCC) and treated with surgery. A total of 165 FFPE tumor samples were analyzed, leading to the identification and quantification of 3091 proteins. Two proteomics subtypes of ccRCC (CC1 and CC2) were identified, each characterized by specific protein expression patterns and functional features. The knowledge gained from this study could potentially aid in the development of new drugs targeting subtype-specific deregulated pathways.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Oncology
Giovanni Cochetti, Jacopo Adolfo Rossi de Vermandois, Vincenza Maula, Luigi Cari, Rosy Cagnani, Chiara Suvieri, Pierfrancesco Maria Balducci, Alessio Paladini, Michele Del Zingaro, Giuseppe Nocentini, Ettore Mearini
Summary: This study aimed to evaluate the diagnostic performance of Bladder EpiCheck and photodynamic diagnosis (PDD) guided cystoscopy in the surveillance of high-risk bladder cancer. It also compared the findings of these tests with urinary cytology to develop a diagnostic algorithm for clinical decision making.
UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
(2022)
Article
Oncology
Giovanni Cochetti, Luigi Cari, Vincenza Maula, Rosy Cagnani, Alessio Paladini, Michele Del Zingaro, Giuseppe Nocentini, Ettore Mearini
Summary: The early diagnosis of ccRCC is crucial for improving patients' survival. A previously developed algorithm based on the level of specific small RNAs in urine showed promising results in predicting the presence of kidney cancer. This study further validated the algorithm's effectiveness and demonstrated its potential application in noninvasive early diagnosis of ccRCC.
Meeting Abstract
Toxicology
A. Maddalon, M. Naghavi, L. Cari, M. Iulini, V. Galbiati, M. Marinovich, G. Nocentini, E. Corsini
TOXICOLOGY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Luigi Cari, Lucrezia Rosati, Giuseppe Leoncini, Eleonora Lusenti, Marco Gentili, Giuseppe Nocentini, Carlo Riccardi, Graziella Migliorati, Simona Ronchetti
Summary: Ulcerative colitis (UC) and Crohn's Disease (CD) are chronic relapsing inflammatory diseases caused by genetic, environmental, and immune factors. The glucocorticoid-induced leucine zipper (GILZ) plays a role in inflammatory bowel disease (IBD) and is associated with genes MUC2, TLR2, and TLR4 in the mucosal barrier. Direct correlations between GILZ/TLR2 and GILZ/TLR4 were found in healthy subjects (HSs) and UC patients, while an inverse correlation was observed between GILZ and MUC2 in HS and IBD patients, particularly those with active disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mahdieh Naghavi Alhosseini, Marianna Palazzo, Luigi Cari, Simona Ronchetti, Graziella Migliorati, Giuseppe Nocentini
Summary: B-acute lymphoblastic leukemia (B-ALL) is a common pediatric cancer. Treg and exhausted CD8(+) T cells play important roles in its development and maintenance. In this study, the expression of 20 Treg/CD8 exhaustion markers was evaluated and their correlation with other factors was analyzed. The results suggest that T cells expressing CD39, CTLA-4, TNFR2, TIGIT, and TIM-3 may promote B-ALL progression and targeted immunotherapy against these markers could be a promising approach for treating B-ALL.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Surgery
Alessio Paladini, Giovanni Cochetti, Graziano Felici, Miriam Russo, Eleonora Saqer, Luigi Cari, Stefano Bordini, Ettore Mearini
Summary: This study aimed to evaluate the intra- and postoperative complications, as well as the oncological and functional outcomes, of robot-assisted radical prostatectomy with pelvic lymph node dissection in patients with high-risk prostate cancer. The results showed that this technique is safe and effective, with only a few low-grade intra- and postoperative complications.
FRONTIERS IN SURGERY
(2023)
Article
Toxicology
Ambra Maddalon, Luigi Cari, Martina Iulini, Mahdieh Naghavi Alhosseini, Valentina Galbiati, Marina Marinovich, Giuseppe Nocentini, Emanuela Corsini
Summary: This study investigated the potential effects of endocrine disruptors (EDs) on immune cells, with a focus on gender differences. The results showed that exposure to different EDs resulted in alterations in RACK1 expression, pro-inflammatory activity, natural killer lytic ability, and lymphocyte differentiation, with gender-specific effects and magnitudes.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Oncology
Cristina Di Giorgio, Rachele Bellini, Antonio Lupia, Carmen Massa, Ginevra Urbani, Martina Bordoni, Silvia Marchiano, Rosalinda Rosselli, Rosa De Gregorio, Pasquale Rapacciuolo, Valentina Sepe, Elva Morretta, Maria Chiara Monti, Federica Moraca, Luigi Cari, Khan Rana Sami Ullah, Nicola Natalizi, Luigina Graziosi, Eleonora Distrutti, Michele Biagioli, Bruno Catalanotti, Annibale Donini, Angela Zampella, Stefano Fiorucci
Summary: This study reveals that the expression and function of FGFR4 in gastric adenocarcinoma is regulated by leukemia inhibitory factor (LIF) in a JAK1/STAT3 dependent manner. LIF and FGF19 converge on the regulation of oncogenic STAT3 in gastric cancer cells.
Meeting Abstract
Pharmacology & Pharmacy
Luigi Cari, Giuseppe Leoncini, Marco Gentili, Lucrezia Rosati, Giuseppe Nocentini, Vincenzo Villanacci, Graziella Migliorati, Carlo Riccardi, Simona Ronchetti
BRITISH JOURNAL OF PHARMACOLOGY
(2023)