期刊
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
卷 67, 期 2, 页码 137-143出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/19396368.2020.1837994
关键词
Microfluidic sperm sorting (MSS); direct swim up; ICSI; MSOME; embryo development
资金
- Shahid Sadoughi University of Medical Sciences, Yazd, Iran
This study compared sperm quality, DNA integrity, embryo development, and clinical outcomes between sperm samples prepared using Microfluidic Sperm Sorting (MSS) and Direct Swim Up (DSU) methods for intracytoplasmic sperm injection (ICSI). The results showed that MSS significantly improved sperm quality parameters and clinical outcomes compared to DSU.
An appropriate preparation technique, should be capable of isolating highquality spermatozoa for intracytoplasmic sperm injection (ICSI). The aim was to assess sperm quality parameters, DNA integrity, embryo development, and clinical outcomes using a practical and accessible Microfluidic Sperm Sorting (MSS) technique. A total of 95 ICSI cases performed using sperm samples were prepared with our MSS (group 1) or by Direct Swim Up (DSU; control) method (group 2). Both sperm quality parameters and sperm DNA fragmentation (SDF) were compared between the groups. DNA fragmentation was assessed using Sperm Chromatin Dispersion (SCD) test and fine morphology was assessed using Motile Sperm Organelle Morphology Examination (MSOME). Embryo development and clinical outcomes were compared between the groups. In the MSS group, progressive motility and the fraction of Class I sperm morphology sperm were significantly higher compared to DSU group (P < 0.01 and P < 0.001, respectively). Moreover, the rates of DNA fragmentation and immotile spermatozoa were significantly lower in MSS when compared to DSU group (P < 0.001). Also, higher rates of high-quality embryo formation (P < 0.001), implantation (P = 0.04) and pregnancy (P = 0.05) were achieved in the MSS compared to DSU groups. The MSS technique proved to be a noninvasive, disposable, easy to use, and inexpensive method for separation of high-quality spermatozoa. Both laboratory parameters and clinical outcomes were improved with application of MSS for neat sperm collection in ICSI.
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