Article
Chemistry, Multidisciplinary
Kaili Zhang, Yuping Zhou, Ziao Xie, Guangpeng Liu
Summary: The color difference between subcutaneous fat (SF) and orbital fat (OF) in humans is likely due to differences in carotenoid and lipid contents. Using UPLC-QE Plus LC-MS/MS analysis, it was found that SF and OF differed in morphology, with lutein being more abundant in SF and potentially responsible for the yellow color of adipose tissue. Lipidomic analysis confirmed the differentiation between SF and OF, providing insights into the metabolism process of lipid droplets in adipocytes.
Article
Microbiology
Paulina Pradel, Nancy Calisto, Laura Navarro, Andres Barriga, Nicolas Vera, Carlos Aranda, Robert Simpfendorfer, Natalia Valdes, Gino Corsini, Mario Tello, Alex R. Gonzalez
Summary: This study identified carotenoids produced by Antarctic soil bacteria, with the pigmented strain CN7 proposed as an alternative model for producing beneficial carotenoid pigments in aquaculture. Characterization and identification of pigment extract were performed through HPLC and LC-MS.
Article
Plant Sciences
Muhammad Zeshan Haider, Adnan Sami, Muhammad Shafiq, Waheed Anwar, Sajid Ali, Qurban Ali, Sher Muhammad, Irfan Manzoor, Muhammad Adnan Shahid, Daoud Ali, Saud Alarifi
Summary: This study identifies 16 CCO genes in rice and demonstrates their importance in response to abiotic stress. Analysis of gene sequences and promoters reveals their relevance to stress response, plant light, development, and hormone responsiveness. Transcriptome data analysis reveals tissue-specific expression patterns of rice CCOs. The study provides valuable insights into the stress resistance mechanisms and breeding of rice.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Dora Zurak, Darko Grbesa, Marija Duvnjak, Goran Kis, Tatjana Medimurec, Kristina Kljak
Summary: The study found that only 43% of total carotenoids in commercial maize hybrids were bioaccessible, with the bioaccessibility of different carotenoids decreasing as the content in the grain increased. While total carotenoid content varied among different hybrids, the majority fell within lower ranges, which should be taken into consideration when formulating animal diets.
Article
Biochemistry & Molecular Biology
Joy Udensi, Ekaterina Loskutova, James Loughman, Hugh J. Byrne
Summary: The study explored the differentiation and quantification of dietary carotenoids using Raman and UV visible absorption spectroscopy. By preprocessing spectral data and modeling analysis, accurate determination of carotenoid content was achieved, suggesting potential application in clinical analysis.
Article
Plant Sciences
Weihua Su, Chang Zhang, Jingfang Feng, Aoyin Feng, Chuihuai You, Yongjuan Ren, Dongjiao Wang, Tingting Sun, Yachun Su, Liping Xu, Niandong Chen, Youxiong Que
Summary: Carotenoid cleavage oxygenases (CCOs) are crucial for plant growth and response to different stresses. This study identified and characterized CCO genes in Saccharum spontaneum and Saccharum spp. R570, providing valuable information for future investigation into their functions. Evolutionary mechanisms and expression patterns of CCO genes in response to various stresses were explored.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Agricultural Engineering
Lisa M. Schuler, Gabriel Bombo, Paulo Duarte, Tamara F. Santos, Ines B. Maia, Filipa Pinheiro, Jose Marques, Rita Jacinto, Peter S. C. Schulze, Hugo Pereira, Luisa Barreira, Joao C. S. Varela
Summary: In this study, two carotenoid-rich strains of Tetraselmis striata CTP4 were isolated through random mutagenesis and selection, showing significantly increased carotenoid and eicosapentaenoic acid contents compared to the wildtype. The upregulation of genes involved in carotenoid biosynthesis in the isolated strains indicates the complexity of regulatory changes required for improving pigment contents in microalgae.
BIORESOURCE TECHNOLOGY
(2021)
Article
Plant Sciences
Yusong Luo, Chaojie Wang, Manman Wang, Yunli Wang, Wenlong Xu, Hongyu Han, Zhichao Wang, Yujuan Zhong, Hexun Huang, Shuping Qu
Summary: Mesocarp color in Cucurbita maxima is determined by carotenoid contents and compositions. This study compared two inbred lines with orange and white fruit flesh colors, revealing the genetic and molecular mechanisms behind their color differences. The findings provide insights for further research on improving the quality of squash through carotenoid biosynthesis.
HORTICULTURAL PLANT JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Joy Udensi, James Loughman, Ekaterina Loskutova, Hugh J. Byrne
Summary: This review discusses the structural and optical properties of carotenoid compounds and the use of Raman spectroscopy to identify carotenoids in biological environments. It focuses on applications in nutritional analysis, optometry, and serology, and explores the progress and challenges in establishing the technique in a clinical environment.
Article
Biochemistry & Molecular Biology
T. Shalini, Sarin S. Jose, P. S. Prasanthi, N. Balakrishna, K. Viswanath, G. Bhanuprakash Reddy
Summary: The study found that diabetic retinopathy (DR) patients had lower plasma carotenoid levels and lower dietary intakes compared to those without DR. Plasma carotenoid levels were negatively associated with the duration of diabetes and blood glucose levels.
Article
Plant Sciences
Fei Du, Zongyu Hu, Lili Qin, Chen Zhang, Zhiwen Wang, Yongchun Shi, Xiaoran Wang, Ran Wang, Yulong Gao, Chen Dong
Summary: This study investigates the role of NtCCD1 in regulating carotenoid content and ROS levels in tobacco leaves. It is found that NtCCD1 cleaves β-carotene and lycopene to produce β-ionone and pseudoionone, leading to an increase in carotenoid contents and a decrease in ROS.
PLANT GROWTH REGULATION
(2023)
Article
Chemistry, Physical
Renata Welc-Stanowska, Rafal Pietras, Bohun Mielecki, Marcin Sarewicz, Rafal Luchowski, Justyna Widomska, Wojciech Grudzinski, Artur Osyczka, Wieslaw I. Gruszecki
Summary: In this study, the antioxidant activity of carotenoids in biomembranes was investigated. The quenching activity of lutein and zeaxanthin in singlet oxygen generation was measured in lipid vesicles using a fluorescent probe and fluorescence lifetime imaging microscopy. The results showed that xanthophylls act as a barrier to the penetration of molecular oxygen into lipid membranes, especially in the 13-cis configuration. This photoisomerization of xanthophylls is a regulatory mechanism important for light filtration and photoprotection.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Miguel Barquin, Ian U. Kouzel, Beat Ehrmann, Michael Basler, Andreas J. Gruber
Summary: This study presents a single cell-based Terminal Exon Annotation database (scTEA-db), which provides yet unannotated terminal exons and associated transcript isoforms for studying their biological role in cell identity and function.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Plant Sciences
Shi Hu, Naofumi Kamimura, Shingo Sakamoto, Soichiro Nagano, Naoki Takata, Sarah Liu, Geert Goeminne, Ruben Vanholme, Mikiko Uesugi, Masanobu Yamamoto, Shojiro Hishiyama, Hoon Kim, Wout Boerjan, John Ralph, Eiji Masai, Nobutaka Mitsuda, Shinya Kajita
Summary: Manipulating cytosolic shikimate recycling can alter the monomeric composition of lignin and enhance enzymatic saccharification in plants.
Article
Biochemistry & Molecular Biology
Zhipeng Qi, Xinyi Tong, Su Bu, Jianjun Pei, Linguo Zhao
Summary: A novel carotenoid cleavage dioxygenase gene from Helianthus annuus was successfully cloned and overexpressed, showing high specificity in cleaving various carotenoids to produce beta-ionone. The study provides a valuable enzyme for the synthesis of natural beta-ionone via biotransformation and synthetic biology platform.
CHEMISTRY & BIODIVERSITY
(2022)