Article
Agriculture, Dairy & Animal Science
Andrea Grana-Baumgartner, Venkata S. R. Dukkipati, Patrick J. Biggs, Paul R. Kenyon, Hugh T. Blair, Nicolas Lopez-Villalobos, Alastair B. Ross
Summary: This study evaluated the impact of short-term cold exposure on new-born lamb brown adipose tissue and plasma lipid composition. The results showed that cold exposure induced significant changes in the lipidome composition, potentially enhancing lipid metabolism and survival during cold adaptation. The study suggests that identifying potential biomarkers for brown adipose tissue activity could improve survival in new-born lambs.
Article
Chemistry, Multidisciplinary
Oliver J. Hale, Helen J. Cooper
Summary: This study demonstrates a mass spectrometry analysis method for the direct analysis of intact oligomeric membrane proteins from tissue. By characterizing protein assembly directly from sheep eye lens sections without sample pre-treatment, our approach allows for simultaneous analysis of soluble protein assemblies.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Spectroscopy
Nickolay S. Medvedev, Nikita A. Orlov, Anatoly I. Saprykin
Summary: A method for analyzing high-purity tellurium using ICP-OES and ICP-MS is proposed, which involves preliminary concentration of trace elements by matrix volatilization, resulting in improved limits of detection.
ATOMIC SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Dehui Xu, Xinying Zhang, Jin Zhang, Rui Feng, Shuai Wang, Yanjie Yang
Summary: This study investigated the effect of plasma-activated water on Pseudomonas aeruginosa and found that it significantly altered bacterial metabolites, inhibiting carbohydrate metabolism and enhancing protein and amino acid decomposition processes. Plasma-activated water could be a promising approach to promote bacterial death.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemical Research Methods
Jian Song, Changbin Yu
Summary: A neural-network-based model Alpha-Tri is proposed to calculate intensity similarity in DIA mass spectrometry, enhancing peptide detection rates and improving performance compared to traditional methods. By learning from the correlation of three spectra, Alpha-Tri demonstrates significant gains in peptide identifications across different datasets.
Article
Biochemical Research Methods
Maria Isabel Alcoriza-Balaguer, Juan Carlos Garcia-Canaveras, Francisco Javier Ripoll-Esteve, Marta Roca, Agustin Lahoz
Summary: In this study, LipidMS 3.0, an R package, was introduced for the whole data analysis workflow from pre-processing to lipid annotation. Compared to existing tools, LipidMS 3.0 performs similarly in data pre-processing performance but provides a higher level of structural information and fewer incorrect annotations in lipid annotation.
Article
Chemistry, Analytical
Yumin Cao, Ning Zhou, Tong Liu, Jinying Zhang, Yongxiang Wang, Bingxian Zhang, Zhenkai Zhang, Weisheng Feng, Xiaoke Zheng
Summary: In this study, a sensitive and rapid ultra-high-performance-tandem mass spectrometry method was developed and applied to determine nine bioactive components in rats after the oral administration of Ephedra herba. The study found that the common cold and nephrotic syndrome could lead to alterations in the pharmacokinetics profiles of these components, providing a reference for further exploration of the pharmacodynamics basis of Ephedra herba.
JOURNAL OF SEPARATION SCIENCE
(2023)
Review
Medical Laboratory Technology
Kenichi Tamama
Summary: Drugs of abuse testing is commonly used in clinical and forensic settings, with urine being the preferred specimen type but other emerging types include saliva, sweat, hair, and meconium. Liquid chromatography-tandem mass spectrometry (LC-tandem-MS) has become the new gold standard for confirmatory drug testing due to its ability to analyze a wider range of analytes compared to gas chromatography-mass spectrometry (GC-MS). Technical advancements such as high-resolution mass spectrometry and miniature ambient ionization mass spectrometry have improved the speed and efficiency of drugs of abuse testing.
CLINICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Linlin Sun, Xueliang Liu, Sihui Liu, Xiaofeng Chen, Zheng Li
Summary: The on-chip strategy combining conductive nanosensors with a chaotic gas micromixer enables real-time monitoring of volatile metabolites from urine for accurate diagnosis and classification of urinary tract cancers.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Madisson Chabas, Olivier Pible, Jean Armengaud, Beatrice Alpha-Bazin
Summary: To accurately and rapidly identify microorganisms from complex microbiota for clinical diagnostic purposes, an innovative label-free multiplexing method for the identification of microorganisms using tandem mass spectrometry is proposed in this paper. The method involves offline reversed-phase fractionation of individual peptidomes and achieves multiplexing by mixing fractions of staged hydrophobicity. This approach enables the identification of up to 21 microorganisms in a single 60-minute run without the use of labeling reagents, providing new perspectives for high-throughput proteotyping of bacteria in large culturomics projects.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Michael S. Westphall, Kenneth W. Lee, Colin Hemme, Austin Z. Salome, Keaton Mertz, Timothy Grant, Joshua J. Coon
Summary: This article describes an apparatus that can cryogenically land particles from the ion beam of a mass spectrometer onto transmission electron microscope grids for cryo-electron microscopy. This system also allows for the controlled formation of thin films of amorphous ice on the grid surface. The study demonstrates that using this cryogenic landing device significantly improves the structural preservation of deposited protein-protein complexes compared to room temperature landings, and landing under cryogenic conditions can increase the diversity of particle orientations, enabling improved 3D structural interpretation.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Martijn M. Vanduijn, Hendrik-Jan Brouwer, Pablo Sanz de la Torre, Jean-Pierre Chervet, Theo M. Luider
Summary: The research team reported an improved electrochemical method that achieves complete reduction of both intermolecular and intramolecular disulfide bridges in a set of monoclonal antibodies. The method utilizes an online electrochemical flow cell to provide information on the pairing of heavy and light chains in the antibodies. The complete reduction of intramolecular disulfide bridges is important for the intact mass of the antibody chain and the confirmation of the protein's amino acid sequence.
ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Laura Rohler, Pernilla Bohlin-Nizzetto, Pawel Rostkowski, Roland Kallenborn, Martin Schlabach
Summary: Long-term monitoring of regulated organic chemicals in ambient air provides valuable information about their environmental fate and trends, essential for evaluating regulatory effectiveness. New suspect and non-target screening analytical strategies are effective in identifying emerging compounds.
ATMOSPHERIC CHEMISTRY AND PHYSICS
(2021)
Article
Endocrinology & Metabolism
Gangqi Wang, Bram Heijs, Sarantos Kostidis, Ahmed Mahfouz, Rosalie G. J. Rietjens, Roel Bijkerk, Angela Koudijs, Lois A. K. van der Pluijm, Cathelijne W. van den Berg, Sebastien J. Dumas, Peter Carmeliet, Martin Giera, Bernard M. van den Berg, Ton J. Rabelink
Summary: In this study, the authors used mass spectrometry imaging, isotope tracing, and multiplexed immunofluorescence microscopy to study metabolic dynamics in the kidney during ischemia-reperfusion. They developed a high-spatial-resolution metabolomics approach that allows mapping of cell-type-specific changes in central carbon metabolism. This method, combined with immunofluorescence staining, can detect metabolic changes and nutrient partitioning in targeted cell types. The authors identified region-specific metabolic perturbations associated with the lesion and throughout recovery, providing insights into the homeostatic capacity of the kidney microenvironment.
Article
Endocrinology & Metabolism
Theodore Alexandrov
Summary: Spatial metabolomics has reached maturity and is driving innovation in mass spectrometry, metabolomics, and spatial omics. It has earned its place in the spatial biology toolbox with exciting discoveries, complementary capabilities, and increasing accessibility, showing promise in biology, medicine, and pharmacology.
Article
Physics, Multidisciplinary
Mohit Verma, Andrew M. Jayich, Amar C. Vutha
PHYSICAL REVIEW LETTERS
(2020)
Article
Multidisciplinary Sciences
Nitesh Chauhan, Andrei Isichenko, Kaikai Liu, Jiawei Wang, Qiancheng Zhao, Ryan O. Behunin, Peter T. Rakich, Andrew M. Jayich, C. Fertig, C. W. Hoyt, Daniel J. Blumenthal
Summary: The study demonstrates highly coherent emission of visible light lasers, providing important tools and technological support for research in atomic, molecular, and optical physics.
NATURE COMMUNICATIONS
(2021)
Article
Physics, Multidisciplinary
C. A. Holliman, M. Fan, A. Contractor, S. M. Brewer, A. M. Jayich
Summary: We report the first operation of a Ra+ optical clock, demonstrating its performance in terms of frequency stability and systematic uncertainty. The clock allows for the measurement of the ratio of Lande ' g-factors and can improve limits on the time variation of the fine structure constant in optical frequency comparisons. Additionally, the clock has features that make it suitable for portable optical clock applications.
PHYSICAL REVIEW LETTERS
(2022)
Article
Multidisciplinary Sciences
Mu Yang, Hao-Qing Zhang, Yu-Wei Liao, Zheng-Hao Liu, Zheng-Wei Zhou, Xing-Xiang Zhou, Jin-Shi Xu, Yong-Jian Han, Chuan-Feng Li, Guang-Can Guo
Summary: This paper explores the tailored topological physics in optical cavities through synthetic dimensions, specifically focusing on a degenerate cavity containing multiple optical angular momenta. The authors have designed and experimentally controlled the coupling among synthetic dimensions, resulting in a periodically driven spin-orbital coupling system. The system's properties were characterized through transmission intensity measurements, revealing the potential for rich topological physics in a compact platform.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Mu Yang, Hao-Qing Zhang, Zheng-Hao Liu, Zheng-Wei Zhou, Xing-Xiang Zhou, Jin-Shi Xu, Yong-Jian Han, Chuan-Feng Li, Guang-Can Guo
Summary: Exceptional points (EPs) are unique spectral features of non-Hermitian systems where multiple eigenvalues and eigenvectors coalesce. We experimentally demonstrate the appearance of paired EPs in a periodic-driven degenerate optical cavity along the synthetic orbital angular momentum dimension with a tunable parameter. The complex-energy band structures and key features of EPs, including bulk Fermi arcs, parity-time symmetry breaking transition, energy swapping, and half-integer band windings, are directly observed through wavefront angle-resolved transmission spectrum. Our results demonstrate the flexibility of using photonic synthetic dimensions to implement non-Hermitian systems beyond their geometric dimension and EP-based sensing.
Article
Optics
Si-Jing Tao, Hao-Qing Zhang, Hao Zhang, Zhe Chen, Ran He, Shao-Jun Dong, Yong-Jian Han, Chuan-Feng Li, Guang-Can Guo
Summary: Tensor-network (TN) states are powerful variational tools for simulating quantum many-body systems. We propose a scheme to generate TN states by combining multiple phonon modes and qubits in the ion trap platform. With the full connectivity and parallelism of the phonon modes operation, we can generate TN states with complex entanglement structures. By refining the local details using the abundant local free parameters of the qubits operation, we benchmark the expressive power of the generated TN states.
Article
Optics
M. Fan, C. A. Holliman, A. Contractor, C. Zhang, S. F. Gebretsadken, A. M. Jayich
Summary: We measured the lifetime of the radium ion's 7p(2)P(3/2) state and found it to be τ = 4.78(3) ns. The measured lifetime is consistent with theoretical calculations and will provide valuable information for determining the differential scalar polarizability of the narrow linewidth 7s S-2(1/2) -> (6)d(2)D(5/2) optical clock transition.
Article
Optics
Hao-Qing Zhang, Ming-Zhong Ai, Jin-Ming Cui, Yong-Jian Han, Chuan-Feng Li, Guang-Can Guo
Summary: The proposal introduces a scheme to realize quantum walk with a single trapped ion for studying topological phenomenon and nonequilibrium dynamics. By tuning parameters and operators, the dynamics of the bound states can be extensively studied to determine different phases.
Article
Optics
C. A. Holliman, M. Fan, A. Contractor, M. W. Straus, A. M. Jayich
Article
Optics
C. A. Holliman, M. Fan, A. M. Jayich