Article
Food Science & Technology
Dan Su, Jiawen Lu, Chunchao Nie, Ziyan Guo, Chang Li, Qiang Yu, Jianhua Xie, Yi Chen
Summary: This study aimed to investigate the effects of AA and OTA individually and collectively on Caco-2 cells. The results showed that AA and OTA significantly inhibited Caco-2 cell viability, decreased TEER values, and increased LY permeabilization, LDH release, and ROS levels. Furthermore, AA and OTA treatment led to increased levels of IL-1 beta, IL-6, and TNF-alpha, while IL-10 levels decreased. Western blot analysis revealed that AA and OTA damaged the intestinal barrier by reducing the expression of tight junction proteins.
Article
Pharmacology & Pharmacy
Masato Maruyama, Yohei Nishida, Hironori Tanaka, Takako Minami, Ken-ichi Ogawara, Masateru Miyake, Yuta Takamura, Hiroki Kakuta, Kazutaka Higaki
Summary: The combined use of spermine and sodium taurocholate enhances the transport of fluorescein isothiocyanate-labeled dextrans across Caco-2 cell monolayers. This enhancement is achieved by increasing membrane fluidity, raising intracellular Ca2+ levels, and rearranging tight junction-constituting proteins. These findings provide insights into the mechanisms underlying the transport enhancement caused by spermine and sodium taurocholate.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Agriculture, Multidisciplinary
Yunika Mayangsari, Natsumi Sugimachi, Wenxi Xu, Chinatsu Mano, Yuki Tanaka, Osamu Ueda, Tomohiro Sakuta, Yoshiharu Suzuki, Yoshinari Yamamoto, Takuya Suzuki
Summary: This study demonstrated that 3,5,7,3',4'- pentamethoxyflavone (PMF) can enhance the intestinal tight junction (TJ) barrier integrity by regulating the transcription of TJ proteins, such as occludin, claudin-3, and claudin-4.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Yunika Mayangsari, Mayu Okudaira, Chinatsu Mano, Yuki Tanaka, Osamu Ueda, Tomohiro Sakuta, Yoshiharu Suzuki, Yoshinari Yamamoto, Takuya Suzuki
Summary: Research showed that DMF can enhance intestinal TJ barrier integrity by increasing the expression of occludin and claudin-1, while reducing the expression of claudin-2. This regulation involves the induction of occludin protein translation via mTOR and silencing Cldn2 mRNA via miR-16-5p.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Oncology
Kana Isayama, Dina Mustika Rini, Yoshinari Yamamoto, Takuya Suzuki
Summary: This study investigated the role of propionate, a major short-chain fatty acid produced by intestinal microorganisms, in the regulation of the intestinal tight junction (TJ) barrier. It was found that propionate strengthened the TJ barrier integrity in human intestinal Caco-2 cells, as indicated by decreased permeability to macromolecules and increased transepithelial electrical resistance. Propionate upregulated ESAM, a TJ-associated protein, and this upregulation was confirmed using various analyses such as qRT-PCR and immunoblotting.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Food Science & Technology
Jae Hoon Lee, Sera Kim, Min-Cheol Kang, Yong Gi Chun, Bum-Keun Kim, Kyung-Mo Song
Summary: This study confirms the protective effect of four nut oils on the damaged intestinal epithelial barrier. Nut oils improve barrier function, inhibit inflammatory response and cytokine production, and enhance the expression and distribution of tight junction proteins in damaged cells.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Environmental Sciences
Ha-Young Park, Jin-Hee Yu
Summary: This study investigated the effects of radiation on TJ-related proteins in an intestinal epithelial cell model. The results showed that X-ray exposure reduced cell viability, proliferation, and growth rate, while increasing intestinal permeability. The expression of TJ-related genes and proteins was also downregulated.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Entomology
Kyong Kim, Eun-Young Park, Dong-Jae Baek, Se-Eun Jang, Yoon-Sin Oh
Summary: Increased tight junction permeability and overproduction of proinflammatory cytokines are crucial in inflammatory bowel disease (IBD). This study evaluated the anti-inflammatory effects of Gryllus bimaculatus extract (AE-GBE) against lipopolysaccharide (LPS)-treated Caco-2 cells, showing that AE-GBE can protect epithelial cells by increasing tight junction proteins and reducing inflammatory mediators.
Article
Food Science & Technology
Xiaoyu Bao, Jianping Wu
Summary: Ovomucin hydrolysate has been shown to prevent pathogen adhesion to intestinal epithelial cells, and in this study, it was demonstrated to improve intestinal barrier integrity by inhibiting inflammatory responses induced by LPS.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Agriculture, Multidisciplinary
Xiaoyu Bao, Jianping Wu
Summary: This study demonstrates the ability of IRW to prevent LPS-induced intestinal barrier dysfunction and inhibit inflammatory responses. IRW mitigates the LPS-induced reduction of intestinal barrier function by restoring the expression and cell surface distribution of the tight junction protein occludin. Furthermore, IRW shows LPS-neutralizing activity and inhibits the activation of NF-KB and MAPK signaling pathways.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Akihiro Watari, Kana Fujiwara, Kiyohito Yagi, Keisuke Tachibana, Takeshi Katsurada, Akira Myoui, Masuo Kondoh
Summary: The study shows that HHT may serve as a granular permeation enhancer by altering the TJ molecules in epidermal cells to enhance transdermal absorption of dextran.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Food Science & Technology
Melissa Fanzaga, Carlotta Bollati, Giulia Ranaldi, Sabrina Sucato, Silvia Fustinoni, Gabriella Roda, Carmen Lammi
Summary: In recent years, there has been a growing interest in exploring alternative delivery systems for iron supplements to improve efficacy and reduce side effects. This study demonstrates the advantages of the Ferro Supremo formulation in improving iron absorption compared to standard FeSO4. The results show that Ferro Supremo can enter, accumulate, and be transported by intestinal cells more efficiently than FeSO4, making it a valuable choice for improving iron deficiency.
Article
Biochemistry & Molecular Biology
Guanzhen Gao, Jingru Zhou, Huiqin Wang, Yanan Ding, Jianwu Zhou, Pik Han Chong, Liying Zhu, Lijing Ke, Xin Wang, Pingfan Rao, Qiang Wang, Longxin Zhang
Summary: The study found that valerate, a SCFAs converted mainly from proteins or amino acids, promotes intestinal barrier function at physiological concentrations, similar to butyrate but with a broader range of effective concentrations. This activity is associated with valerate-induced AMPK activation and tight junctions assembly, highlighting its potential role in intestinal health.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Agriculture, Multidisciplinary
Dina Mustika Rini, Yusuke Nakamichi, Tomotake Morita, Hiroyuki Inoue, Yoichi Mizukami, Yoshinari Yamamoto, Takuya Suzuki
Summary: This study revealed that xylobiose suppresses Claudin 2 expression and increases HSP27 expression in intestinal Caco-2 cells via post-transcriptional regulation, potentially strengthening intestinal barrier integrity. However, these effects seem to occur via different signaling pathways. Our findings may help to assess the physiological role of xylobiose.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Multidisciplinary Sciences
Jan-Leo Kaak, Fabia D. Lobo de Sa, Jerrold R. Turner, Joerg-Dieter Schulzke, Roland Buecker
Summary: In this study, we investigated the mechanism by which microcystin leads to diarrhea. Our results showed that microcystin compromises the integrity of the intestinal epithelial barrier, resulting in increased permeability and diarrhea.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)