期刊
DOSE-RESPONSE
卷 14, 期 1, 页码 -出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/1559325816632102
关键词
ferritin; nanoparticles; salt-dependent; aggregation; self-assembling
资金
- National Nature Scientific Foundation of China [81200145]
Ferritin, with the primary function of iron storage, is a nearly ubiquitous protein found in most living organisms. Our recent investigations suggest that ferritin can assemble nanoparticles. So we use ferritin as a novel type of delivery vehicle for recombinant epitope vaccines. And, we found that ferritin form nonnative aggregates depended sensitively on NaCl concentrations. Here, we report that ferritin is an ion-sensitive protein and has the attribute of salt-dependent aggregation. Our results indicate that recombinant ferritin can be released as a soluble form from Escherichia coli at low NaCl concentrations (50 mmol/L). Moreover, this result affords us to confirm a proper self-assembling solution for soluble ferritin or other ferritin-based fusion proteins to assemble nanoparticles.
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