期刊
NEW PHYTOLOGIST
卷 229, 期 5, 页码 2984-2997出版社
WILEY
DOI: 10.1111/nph.17060
关键词
Dcl5; DNA methylation; Ms23; phasiRNA; Zea mays (maize)
资金
- US National Science Foundation Plant Genome Research Program (NSF-PGRP) [1649424]
- International Postdoctoral Exchange Fellowship Program [20140067]
- US National Science Foundation [DBI-1237931]
- Chinese Academy of Sciences [Y971RG1001]
- NCBI's Short Read Archive under BioProject [PRJNA639640]
In maize, the 24-nt phasiRNAs are essential for targeting increased DNA methylation at specific loci in anthers, and not just for transcriptional activation of PHAS precursors. Mutants of male sterile23 and Dicer-like5 genes lack sufficient 24-nt phasiRNAs, showing the importance of these small RNA molecules in regulating male fertility.
Plant phased small interfering RNAs (phasiRNAs) contribute to robust male fertility; however, specific functions remain undefined. In maize (Zea mays), male sterile23 (ms23), necessary for both 24-nt phasiRNA precursor (24-PHAS) loci and Dicer-like5 (Dcl5) expression, and dcl5-1 mutants unable to slice PHAS transcripts lack nearly all 24-nt phasiRNAs. Based on sequence capture bisulfite-sequencing, we find that CHH DNA methylation of most 24-PHAS loci is increased in meiotic anthers of control plants but not in the ms23 and dcl5 mutants. Because dcl5-1 anthers express PHAS precursors, we conclude that the 24-nt phasiRNAs, rather than just activation of PHAS transcription, are required for targeting increased CHH methylation at these loci. Although PHAS precursors are processed into multiple 24-nt phasiRNA products, there is substantial differential product accumulation. Abundant 24-nt phasiRNA positions corresponded to high CHH methylation within individual loci, reinforcing the conclusion that 24-nt phasiRNAs contribute to increased CHH methylation in cis.
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