期刊
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
卷 33, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.nano.2020.102353
关键词
Composite biomaterial; Skin wound healing; Collagen electrospinning; Hydroxyapatite electrospraying; Calcium ions
资金
- FundacAo para a Ciencia e a Tecnologia, Portugal [SFRH/BD/69686/2010, UID/BIM/04293/2013/POCI010145FEDER007274]
- i3S Scientific Platform Bioimaging [PPBIPOCI010145FEDER022122]
- i3S Scientific Platform BioSciences Screening [PPBIPOCI010145FEDER022122]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/69686/2010] Funding Source: FCT
This study utilized a composite matrix for skin regeneration applications, showing that calcium ions provided by nanoHA significantly enhanced cellular growth and proliferation rates while preventing adhesion of pathogenic bacteria. The mechanically fit composite membrane also showed no adverse reactions in rat implantation experiments.
This study reflects an exploitation of a composite matrix produced by electrospinning of collagen and electrospraying of nanophased hydroxyapatite (nanoHA), for skin regeneration applications. The main goal was to evaluate the effect of nanoHA, as source of localized calcium delivery, on human dermal fibroblasts, keratinocytes, and human mesenchymal stem cells (hMSCs) growth, proliferation, differentiation, and extracellular matrix production. This study revealed that calcium ions provided by nanoHA significantly enhanced cellular growth and proliferation rates and prevented adhesion of pathogenic bacteria strains typically found in human skin flora. Moreover, hMSCs were able to differentiate in both osteogenic and adipogenic lineages. Rat subcutaneous implantation of the membranes also revealed that no adverse reaction occurred. Therefore, the mechanically fit composite membrane presents a great potential to be used either as cell transplantation scaffold for skin wound regeneration or as wound dressing material in plastic surgery, burns treatment or skin diseases. (c) 2021 Elsevier Inc. All rights reserved.
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