期刊
MOLECULAR BIOLOGY REPORTS
卷 48, 期 1, 页码 989-995出版社
SPRINGER
DOI: 10.1007/s11033-020-06088-w
关键词
Single nucleotide polymorphism (SNP); Rainbow trout; Next generation sequencing; Transcriptome
资金
- Iran National Science Foundation (INSF) [95841911]
In this study, 52 novel single nucleotide polymorphism (SNP) markers for Rainbow trout (Onchorhynchus mykiss) were discovered and validated, which could be useful for future genetic studies and breeding programs. The findings suggest that these markers are important for detecting adaptive signals and parentage assignment, and can also contribute to improving economic traits and management of the species.
Rainbow trout (Onchorhynchus mykiss) is one of the most important freshwater aquaculture fish in Iran. It is necessary to develop available molecular marker such as SNPs, which represent a useful tool in detecting adaptive signals in populations and also parentage assignment for O. mykiss. Genetic architecture of broodstock populations is important for breeding programs, as it enables decisions on broodstock screening and genomic selection. In this study, 52 novel single nucleotide polymorphism (SNP) markers for O. mykiss were discovered and validated based on transcriptome sequencing, by means of paired-end sequencing in an Illumina HiSeq 2500 platform. The SNPs were identified through liver transcriptome sequencing from fifteen samples. The observed and expected heterozygosities ranged from 0.177 to 1.000 and 0.239 to 0.638, respectively. The minimum allele frequency (MAF) ranged from 0.166 to 0.489. Among these SNP loci, twenty-two loci showed significant departures from the Hardy-Weinberg equilibrium after Bonferroni correction (p < 0.05) and significant linkage disequilibrium was found. The SNP markers identified in this research could be useful for novel studies, such as those related to associations between high-resolution molecular markers and quantitative traits studies. Moreover, these SNP markers would be used in genetic studies helping economic performance improvement and management of this species.
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