Article
Immunology
Ying-da Wang, Jin-shuo Gong, Yong-chao Guan, Ze-lin Zhao, Ya-nan Cai, Xiao-feng Shan
Summary: In this study, the regulatory effect of ompR gene on the biological characteristics and virulence of A. veronii TH0426 was investigated. Mutant and complement strains were constructed, and the results showed that the ompR gene deletion significantly reduced biofilm formation, osmotic stress resistance, and drug resistance of TH0426. Animal pathogenicity experiments also demonstrated a significant decrease in virulence. These findings indicate that ompR gene plays a role in regulating biofilm formation, drug sensitivity, osmotic stress resistance, and virulence of TH0426.
MICROBIAL PATHOGENESIS
(2023)
Article
Fisheries
Lei Zhang, Shengnan Jin, Chao Feng, Haichao Song, Sayed Haidar Abbas Raza, Huabo Yu, Liang Zhang, Teng Chi, Yanling Qi, Dongxing Zhang, Aidong Qian, Ning Liu, Xiaofeng Shan
Summary: This study constructed a mutant strain to investigate the functional role of aodp gene in A. veronii TH0426, revealing that aodp deficiency leads to changes in growth rate, drug resistance, swimming ability, pathogenicity, and caspase activity of the bacterium. The down-regulation of 17 genes related to virulence due to aodp deficiency provides insights for further studies on the pathogenesis of A. veronii.
JOURNAL OF FISH DISEASES
(2022)
Article
Immunology
Hong Wang, Ze Lin-Zhao, Ding Jie-An, Jing Lin-Wang, Bin Tong-Yang, Yuan Huan-Kang, Dong Xing-Zhang, Hai Chao-Song, Xiao Feng-Shan, Ai Dong-Qian
Summary: Aeromonas veronii (A. veronii) is a pathogen that can infect aquatic organisms and mammals. This study found that the mutant strain Delta lip led to a decrease in the adhesion and virulence of the wild-type strain.
MICROBIAL PATHOGENESIS
(2022)
Article
Fisheries
Ying-da Wang, Jin-shuo Gong, Yong-chao Guan, Ze-lin Zhao, Ya-nan Cai, Xiao-feng Shan
Summary: In this study, mutant and complemented strains of A. veronii TH0426 were constructed to investigate the impact of the hcp1 gene on the bacterial virulence. Deletion of the hcp1 gene resulted in flagella fracture and affected basic biological characteristics and pathogenicity of TH0426 cells. The study demonstrates that the hcp1 gene regulates flagella assembly and plays a role in the animal pathogenesis of TH0426.
Article
Microbiology
Vera Irene Erickson, Ahmed Alfifi, Yaovi Gildas Mahuton Hounmanou, Mohammad Jafar Sana, Jens Peter Christensen, Anders Dalsgaard
Summary: A whole genomic analysis of 24 strains of Aeromonas veronii from a Danish abattoir revealed their widespread distribution in human, aquatic, and poultry samples, carrying various virulence factors and showing potential clinical and veterinary importance. Further epidemiological studies are needed to determine its pathogenicity in poultry.
VETERINARY MICROBIOLOGY
(2023)
Article
Fisheries
Ruoming Li, Jiaxin Tian, Qiumei Shi, TongLei Wu, Dingjie An, Xiaofeng Shan, Guiqin Wang
Summary: The deletion of the cadA gene in A. veronii significantly decreased its virulence and adhesion while increasing biofilm formation ability.
AQUACULTURE REPORTS
(2021)
Article
Immunology
Hongmiao Gao, Hong Wang, Meng Zhang, Xiaofeng Shan, Aidong Qian, Lei Zhang, Dongxing Zhang, Wuwen Sun
Summary: This study found that deleting the lsrB gene reduces the virulence of Aeromonas veronii, resulting in decreased toxicity and adhesion ability, as well as impaired swimming ability.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Hong Li, Xiang Ma, Yanqiong Tang, Dan Wang, Ziding Zhang, Zhu Liu
Summary: This study constructed a genome-wide PPI network for Aeromonas veronii and found that its virulence factors had higher degree and betweenness centrality, tended to interact with each other, and were enriched in specific modules. The virulence factors may regulate A. veronii's virulence through signal transduction pathways and manipulate host biological processes by mimicking and competitively binding to host proteins.
Article
Microbiology
Yongchao Guan, Meng Zhang, Yingda Wang, Zhongzhuo Liu, Zelin Zhao, Hong Wang, Dingjie An, Aidong Qian, Yuanhuan Kang, Wuwen Sun, Xiaofeng Shan
Summary: In this study, a mutant strain of Aeromonas veronii was constructed, and the deletion of the ascP gene resulted in down-regulation of associated effector proteins, decreased adhesive and invasive abilities, as well as attenuated toxicity and virulence. The ascP gene was found to play an essential role in the etiopathogenesis of A. veronii TH0426.
JOURNAL OF MICROBIOLOGY
(2022)
Article
Microbiology
Bijay Kumar Behera, Satya Narayan Parida, Vikash Kumar, Himanshu Sekhar Swain, Pranaya Kumar Parida, Kampan Bisai, Souvik Dhar, Basanta Kumar Das
Summary: This study investigated a case of severe mortality in farmed Labeo rohita and identified the causative agent as Aeromonas veronii. The isolated strain showed resistance to ampicillin and dicloxacillin, but susceptibility to 22 other antibiotics. It was also found that A. veronii induced stress and immune responses in L. rohita. The negative effects of the pathogen on fish health and high mortality rate highlight the need for A. veronii management.
Article
Microbiology
Yanelys Cantillo Villa, Adriana Triga, Pantelis Katharios
Summary: This study investigated the characteristics of two Aeromonas strains and found that A. veronii bv sobria strain is the primary pathogen responsible for European seabass aeromoniasis. Additionally, when co-administered with A. rivipollensis, A. veronii bv sobria strain exhibited antagonistic interaction.
Article
Fisheries
Shengping Li, Shun Zhou, Qiuhong Yang, Yongtao Liu, Yibin Yang, Ning Xu, Xiaohui Ai, Jing Dong
Summary: A study found that cinnamaldehyde, extracted from a natural plant, has inhibitory effects on Aeromonas hydrophila infection. Cinnamaldehyde disrupts the bacterial quorum sensing system, reducing the pathogenic behaviors of A. hydrophila without affecting bacterial growth. It was also observed that cinnamaldehyde downregulates the expression of related genes and can effectively decrease host cell injury and increase the survival rate in A. hydrophila-infected hosts. These results suggest that cinnamaldehyde could be a potential candidate as a quorum sensing inhibitor against A. hydrophila infection.
Article
Fisheries
Tong Zhao, Bintong Yang, Hongjin Li, Aidong Qian, Wei Cong, Wuwen Sun, Yuanhuan Kang
Summary: The mutation of the ascO gene in A. veronii reduces its adhesion and toxicity to EPC cells, as well as the level of apoptosis induced by the bacteria. These findings are significant for further understanding the function of the ascO gene and the pathogenesis of A. veronii.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Zhao Cheng, Xianliang Zhao, Xinyu Jiang, Jie Zhang, Li Li, Chao Pei, Yong Zhou, Lingbing Zeng, Xianghui Kong
Summary: Aeromonas veronii is an important zoonotic and aquatic pathogen. This study found that the mutation of its flagellar cap gene (fliD) led to reduced motility, defective flagellum, and attenuated biofilm formation. In vivo experiments showed that the mutant strain had lower colonization capacity and higher median lethal dose compared to the wild-type strain.
JOURNAL OF FISH DISEASES
(2023)
Article
Biotechnology & Applied Microbiology
Shengping Li, Qiuhong Yang, Bo Cheng, Yongtao Liu, Shun Zhou, Xiaohui Ai, Jing Dong
Summary: Neem oil was found to inhibit the quorum-sensing of A. hydrophila and reduce the production of various virulence factors at sub-inhibitory concentrations. Real-time PCR confirmed that neem oil could regulate the transcription of relevant genes, and it exhibited significant protection in cell and animal models.
Article
Agriculture, Dairy & Animal Science
Li Zhou, Sayed Haidar Abbas Raza, Boyan Ma, Abdullah F. Shater, Zuhair M. Mohammedsaleh, Ali Raza Jahejo, Jiangwei Li, Linsheng Gui
Summary: Four SNPs were identified in Tibetan sheep, and the g.49400A > G and g.49587A > T of FGFR1 were found to be associated with growth-related traits.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Sayed Haidar Abbas Raza, Rajwali Khan, Sameer D. Pant, Mujahid Ali Shah, Guobo Quan, Long Feng, Gong Cheng, Lin-sheng Gui, Linsen Zan
Summary: This study investigated the association between OPN genotypes and haplotypes with milk composition in Chinese Holstein cows. The results showed that certain haplotypes and genetic markers were associated with increased butter-fat percentage, suggesting that the polymorphisms in OPN could be potentially used for marker-assisted selection in Chinese Holstein cows.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Sihu Wang, Sayed Haidar Abbas Raza, Ke Zhang, Chugang Mei, Muna O. Alamoudi, Bandar H. Aloufi, Ahmed Mohajja Alshammari, Linsen Zan
Summary: Qinchuan cattle have undergone gradual improvements in body shape and growth rate through long-term breeding. Whole genome sequencing data revealed candidate genes related to the growth and development traits of Qinchuan cattle, including brain size, body characteristics, and limb development. Other genes were also found to be associated with meat quality, reproductive function, and coat color. These findings provide insights into breed formation and molecular genetic breeding, and can contribute to improving animal production and health.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Boyan Ma, Rajwali Khan, Sayed Haidar Abbas Raza, Zhanhong Gao, Shengzhen Hou, Farman Ullah, Montaser M. Hassan, Mohamed M. Hassan, Qwait AlGabbani, Mashael Alhumaidi Alotaibi, Mujahid Ali Shah, Linsheng Gui
Summary: This study identified three mutations in the SIRT6 and SIRT7 genes in black Tibetan sheep, with one mutation in SIRT6 associated with body weight and heart girth, and one mutation in SIRT7 associated with body weight. These findings can serve as genetic markers and guides for breeding in black Tibetan sheep.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Mei Liu, Jie Cheng, Yuhan Chen, Long Yang, Sayed Haidar Abbas Raza, Yongzhen Huang, Chuzhao Lei, George E. Liu, Xianyong Lan, Hong Chen
Summary: This study revealed the distribution of DGAT1 CNV in Chinese goats for the first time and found significant differences among dairy, meat, and fiber goats. The study also showed significant associations between DGAT1 CNV and milk production traits, indicating its potential role in marker-assisted selection of dairy goat breeding.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Kaster Nurgulsim, Rajwali Khan, Sayad Haidar Abbas Raza, Amel Ayari-Akkari, Mouna Jeridi, Ijaz Ahmad, Uroosa Mumtaz Arain, Ayman Hassan Abd El-Aziz, Hamayun Khan, Linsen Zan
Summary: This study identifies significant associations between SNPs in the FGF10 gene and body measurement and carcass quality traits in Qinchuan beef cattle, showing high levels of polymorphism.
ANIMAL BIOTECHNOLOGY
(2023)
Review
Veterinary Sciences
Atif Khurshid Wani, Nahid Akhtar, Reena Singh, Ajit Prakash, Sayed Haidar Abbas Raza, Simona Cavalu, Chirag Chopra, Mahmoud Madkour, Ahmed Elolimy, Nesrein M. Hashem
Summary: Livestock is crucial in modern agriculture, and genome editing techniques have been developed to improve economic traits and disease resistance, contributing to sustainable livestock production.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Jiqian Liu, Lijuan Han, Wenzheng Han, Linsheng Gui, Zhenzhen Yuan, Shengzhen Hou, Zhiyou Wang, Baochun Yang, Sayed Haidar Abbas Raza, Abdulaziz Faisal Saleh Alowais, Alaa Ahmed Alraddadi, Anwar Mohammed Alanazi
Summary: This study investigated the effect of different heat treatments (pan-frying, deep-frying, baking, and boiling) on the quality and flavor of Black Tibetan sheep meat. The results showed that pan-fried meat had the highest sensory scores and nutritional content, including protein and amino acid. It also contained higher amounts of monounsaturated and polyunsaturated fatty acids. Additionally, pan-fried and deep-fried meat had higher levels of volatile flavor compounds compared to baked meat. The study concluded that pan-fried Black Tibetan sheep meat had the best overall quality.
Review
Microbiology
Yufan Xu, Shiwei Ma, Zixin Huang, Longlong Wang, Sayed Haidar Abbas Raza, Zhe Wang
Summary: This paragraph mainly discusses the importance of nitrogen metabolism on the survival and virulence of Mycobacterium tuberculosis. However, research on nitrogen metabolism is limited compared to carbon metabolism. Although various small molecule inhibitors and drugs have been developed, long-term validation is needed before their practical application.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Genetics & Heredity
Wenzhen Zhang, Sayed Haidar Abbas Raza, Bingzhi Li, Bing Sun, Sihu Wang, Sameer D. D. Pant, Nouf S. S. Al-Abbas, Nehad A. A. Shaer, Linsen Zan
Summary: This study found that miR-33a can inhibit the differentiation of bovine preadipocytes by suppressing the IRS2-Akt pathway, leading to reduced lipid droplet accumulation and decreased expression of adipocyte marker genes.
Article
Agriculture, Dairy & Animal Science
Hengwei Yu, Sayed Haidar Abbas Raza, Yueting Pan, Gong Cheng, Chugang Mei, Linsen Zan
Summary: This study used blood transcriptome sequencing and metabolite detection to explore the differential genes and metabolites associated with the subcutaneous fat depot phenotype in beef cattle. The functional genes SMPD3 and CERS1, as well as the metabolite sphingosine 1-phosphate, were identified as important factors contributing to the differences in phenotype. These findings provide important reference value for effective breeding to improve beef performance.
Article
Food Science & Technology
Ying Ma, Lijuan Han, Sayed Haidar Abbas Raza, Linsheng Gui, Xue Zhang, Shengzhen Hou, Shengnan Sun, Zhenzhen Yuan, Zhiyou Wang, Baochun Yang, Mohamed M. Hassan, Ruqaih S. Alghsham, Waleed Al Abdulmonem, Samia S. Alkhalil
Summary: This study investigated the effects of Palm kernel meal (PKM) on the quality and flavor of Tibetan sheep meat. The results showed that Tibetan sheep in the group supplemented with 18% PKM exhibited superior eating quality and flavor profile, along with increased protein and fat deposition. Metabolomics analysis revealed that PKM feed affected meat metabolites and metabolic pathways. Furthermore, PKM supplementation also influenced the composition of rumen microorganisms, which in turn regulated meat quality. Overall, adding PKM can improve the quality and flavor of meat through its effects on muscle metabolism and rumen microorganisms.
FOOD SCIENCE & NUTRITION
(2023)
Review
Infectious Diseases
Shuang Liang, Yanling Qi, Huabo Yu, Wuwen Sun, Sayed Haidar Abbas Raza, Nada Alkhorayef, Samia S. Alkhalil, Essam Eldin Abdelhady Salama, Lei Zhang
Summary: Antibiotic resistance is a global issue that needs to be addressed. Bacteriophage, unlike antibiotics, can selectively kill target bacteria without affecting normal bacterial flora. This review describes the biological characteristics of phage and its application in China, including veterinary and human clinical treatment. Although phage has shown therapeutic potential, challenges such as narrow lysis spectrum, immune response, storage issues, and lack of unified regulations hinder its widespread use as a substitute for antibiotics. The aim is to highlight the advancing field of phage therapy and the challenges China faces in reducing antibiotic reliance.
Article
Biology
Sayed Haidar Abbas Raza, Ruimin Zhong, Shen Xing, Xiaoting Yu, Liang Chengcheng, Linsen Zan, Nicola M. Schreurs, Sameer D. Pant, Hongtao Lei
Summary: The purpose of this study was to identify potential RNA binding proteins associated with the survival of gastric adenocarcinoma and investigate their biological characteristics and signaling pathways. A total of 1495 RNA binding protein related genes were analyzed for differential expression, resulting in 296 differential RNA binding proteins. Cox regression analysis identified 20 prognosis-related RNA binding proteins, from which a new prognostic model was constructed. The model was validated using survival analysis, receiver operating characteristic curves, and prognosis analysis curves, and a prediction nomogram with good performance was developed.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Veterinary Sciences
Dianqi Zhang, Sayed Haidar Abbas Raza, Xinze Du, Juze Wang, Meng Wang, Jing Ma, Kuncheng Xie, Sameer D. Pant, Jie He, Bander Hamad Aloufi, Chugang Mei, Linsen Zan
Summary: This study found that substituting alfalfa hay with corn silage as feed had a negative impact on the fertility of breeding bulls. Feeding corn silage decreased semen quality by reducing sperm motility and increasing deformity rate. It also affected the diversity of rumen microbiota, which was associated with semen quality. Overall, this study suggests that using corn silage as the primary source of forage could negatively affect semen quality in breeding bulls.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
