Article
Biochemistry & Molecular Biology
Fa Dai, Ming Guo, Yina Shao, Chenghua Li
Summary: The study revealed that the flagellum protein of Vibrio splendidus destroyed tissue structure and promoted apoptosis in sea cucumber, with the novel receptor AjTmod playing a role in activating the p38 MAPK signaling pathway to induce p53-dependent coelomocyte apoptosis in Echinodermata.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Fisheries
Ya Li, Fa Dai, Yanan Li, Weikang Liang, Chenghua Li, Weiwei Zhang
Summary: Hfq, as an RNA-binding protein, plays a crucial role in regulating the virulence of Vibrio splendidus by affecting adhesion, biofilm formation, and swimming motility through the flagellar assembly and chemotaxis systems. The construction of a Delta hfq mutant strain revealed reduced growth and virulence levels, with transcriptomic analysis showing significant down-regulation of key genes in these pathways. The study highlights the importance of Hfq in modulating virulence-related factors in V. splendidus.
Article
Biotechnology & Applied Microbiology
Huirong Yang, Huimin Song, Jinxia Zhang, Weisheng Li, Qingxi Han, Weiwei Zhang
Summary: Vibrio splendidus, a ubiquitous Gram-negative marine bacterium, was studied to explore the effect of iron deprivation on its proteomic profile. The results showed that 425 differentially expressed proteins responded to the iron deprivation condition. Under iron deprivation, the oxidation-reduction processes, single-organism metabolic processes, catalytic activity, and binding activity were downregulated, while transport process, membrane cell component, and ion binding activity were upregulated. The study provides basic information on the global protein profiles of V. splendidus under iron deprivation condition and suggests that it accelerates the cell growth to higher cell density.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Fisheries
Zhenguo Hu, Weiwei Zhang, Weikang Liang, Zhen Zhang, Ming Guo, Chenghua Li
Summary: A bacterium identified as Bacillus cereus LS2 isolated from healthy sea cucumbers showed strong antagonistic activity against Vibrio splendidus, potentially through the production of a lipopeptide. The antagonistic substance had a molecular weight less than 3 kDa, was sensitive to trypsin but stable at high temperatures, and could be precipitated by acid and dissolved in anhydrous methanol.
Article
Microbiology
Ya Li, Weibo Shi, Weiwei Zhang
Summary: This study analyzed the effects of exogenous L-Glu on the viability and tissue status of sea cucumbers, and found that high concentrations of L-Glu resulted in cell death and tissue damage, while low concentrations had no significant impact on the survival of sea cucumbers. Transcriptomic analysis revealed that exogenous L-Glu affected the expression of multiple genes, and coexistence of exogenous L-Glu and V. splendidus further increased sea cucumber mortality.
Article
Cell Biology
Jiejie Sun, Xiaoqian Lv, Jinyuan Leng, Lingling Wang, Linsheng Song
Summary: In this study, researchers identified a complete mitophagy pathway in the Pacific oyster by screening the components of the pathway and comparing them to those found in humans. This discovery contributes to the understanding of mitochondrial autophagy in mollusks.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Shanshan Zhang, Qian Yang, Songzhe Fu, Colin R. Janssen, Mieke Eggermont, Tom Defoirdt
Summary: This study demonstrates that indole signaling has a significant impact on the virulence of Vibrio tasmaniensis LGP32 and Vibrio crassostreae J2-9, affecting their motility, biofilm formation, and gene expression. Pretreatment with indole decreases bacterial virulence towards mussel larvae, indicating that indole signaling could be a promising target for antivirulence therapy.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Yang Li, Hongqing Yao, Sushuang Liu, Dawei Song, Choufei Wu, Shaoyong Zhang, Quanxin Gao, Liqin Zhang
Summary: This study investigated the ability of flagellins from V. parahaemolyticus (flaA, flaB, flaC, flaD, flaE, and flaF) to induce apoptosis in a fish cell line. All six flagellins induced severe apoptosis and increased TLR5 and MyD88 expression as well as TNF-α and IL-8 production, indicating a TLR5-mediated immune response via an MyD88-dependent pathway. FlaF exhibited the strongest immunostimulatory effect and was found to directly bind to TLR5. Molecular simulation identified the amino acids involved in the TLR5-flaF interaction. These findings enhance our understanding of the immunogenic properties of flagellins from V. parahaemolyticus and their potential for vaccine development.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Fisheries
Ya Li, Huirong Yang, Jinxia Zhang, Weibo Shi, Weisheng Li, Weiwei Zhang
Summary: This study focused on Vibrio splendidus, a marine pathogen that infects various aquaculture animals. The researchers cloned the catalytic domain of the vspC gene and successfully purified the recombinant catalytic domain rVspC. They found that rVspC can degrade collagen from the body wall and coelomocytes of Apostichopus japonicus. Transcriptomics analysis revealed gene expression changes in A. japonicus injected with rVspC, including upregulation of MMP-14 and downregulation of laminin. Immune-related pathways were affected, with downregulation of apoptosis, lysosome, phagosome, ubiquitin-mediated proteolysis, and antigen processing and presentation, and upregulation of endocytosis. The study also identified a regulatory pathway of Hfq-SmcR-VspC at high cell density of V. splendidus, indicating the positive regulation of SmcR by Hfq and its role in the expression of the vspC gene. Furthermore, the comparative analysis of VspC's catalytic domain sequences suggests the widespread presence of collagenase homologs in bacteria, potentially playing important roles in colonization, environmental adaptation, and virulence.
Article
Fisheries
Yingfen Dai, Zhimeng Lv, Meixiang You, Lianlian Sun, Chenghua Li
Summary: Organisms trigger pro-inflammatory responses to resist foreign pathogens, but excessive or chronic inflammation can cause diseases. This study found that the PPAR alpha gene in sea cucumbers was significantly induced at the late stage of infection and negatively correlated with AjIL-17 and AjNLRP3 expression. The results supported that AjPPAR alpha exerted anti-inflammatory effects through inhibiting AjRel.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Fangyuan Zhou, Lianlian Sun, Yina Shao, Xiumei Zhang, Chenghua Li
Summary: Glutaminolysis plays an important role in the immune response in Apostichopus japonicus. Decreased levels of glutaminolysis metabolic substrates and products were observed, accompanied by an increase in glutathione. The key genes in glutaminolysis were upregulated in response to pathogen infection. AMPK beta can regulate glutaminolysis by influencing the uptake of glutamine. Silencing AjAMPK beta leads to increased levels of glutamine and glutamate, decreased production of glutathione, and a reduction in redox homeostasis indexes.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Veterinary Sciences
Tian-Yang Hu, Yi-Bing Lian, Jia-Hao Qian, Yong-Lei Yang, Emad Beshir Ata, Rong-Rong Zhang, Chun-Wei Shi, Gui-Lian Yang, Hai-Bin Huang, Yan-Long Jiang, Jian-Zhong Wang, Xin Cao, Nan Wang, Yan Zeng, Wen-Tao Yang, Chun-Feng Wang
Summary: Avian influenza virus is highly pathogenic in birds and humans, causing significant economic losses to poultry farms and public health problems. Vaccination is an important strategy to control the spread of avian influenza. This study showed that recombinant L. plantarum can activate immune responses, promote cell proliferation, and induce the production of protective antibodies in mice, indicating its potential as a vaccine for controlling AIV infection.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Fisheries
Kaiyu Chen, Yina Shao, Chenghua Li
Summary: The sea cucumber AjULK was found to be significantly upregulated after pathogen challenge and involved in autophagy regulation along with other autophagy-related genes. Knockdown of AjULK markedly inhibited the autophagy signal and affected protein levels inside cells, ultimately restricting bacterial invasion.
Article
Biochemistry & Molecular Biology
Quanxin Gao, Shaokui Yi, Jinping Luo, Qianqian Xing, Jiali Lv, Panhuang Wang, Cuihua Wang, Yang Li
Summary: By knocking out the FlaB gene of Vibrio anguillarum, this study found little impact on the induction of immune-related gene expression, and the mutant displayed similar flagella morphology and immune characteristics to the wild-type strain. This suggests that knockout of FlaB may promote the expression and function of other flagellin proteins.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Immunology
Siyuan Zhang, Yina Shao, Chenghua Li
Summary: The study characterized the involvement of lncRNAs in sea cucumber upon bacterial infection, showing that some lncRNAs may act as regulators in pathogen-host interactions by sponging miRNAs.
FRONTIERS IN IMMUNOLOGY
(2021)
