Review
Immunology
Ping Zheng, Fang Liu, Jinzhao Long, Yuefei Jin, Shuaiyin Chen, Guangcai Duan, Haiyan Yang
Summary: Staphylococcus aureus is a significant human pathogen that can cause a wide range of diseases. Conventional mouse studies lack predictive ability due to the differences in immune systems between mice and humans. Humanized mice provide a promising model for studying human-specific virulence factors and the interaction mechanisms of S. aureus with humans.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Jumpei Fujiki, Tomohiro Nakamura, Keisuke Nakamura, Keita Nishida, Yurika Amano, Yusaku Watanabe, Satoshi Gondaira, Masaru Usui, Masaru Shimizu, Kazuhiko Miyanaga, Shinya Watanabe, Tomohito Iwasaki, Kotaro Kiga, Tomoko Hanawa, Hidetoshi Higuchi, Teiji Sawa, Yasunori Tanji, Yutaka Tamura, Longzhu Cui, Hidetomo Iwano
Summary: The study assessed the biological stability and clinical potential of Staphylococcus virus Phi SA012. The virus showed a wide host range and efficient lytic activity. Experiments on mice demonstrated the ability of Phi SA012 to spread and accumulate in specific organs without causing inflammation. Additionally, the study highlighted the importance of appropriate dosing and administration periods for effective phage therapy.
SCIENTIFIC REPORTS
(2022)
Article
Food Science & Technology
Fei Ma, Yu Ning, Qingqing Wan, Likou Zou, Yuntao Liu, Shujuan Chen, Jianlong Li, Zhen Zeng, Yong Yang, Hong Chen, Min Zhou, Shuliang Liu, Cheng Li, Aiping Liu
Summary: Bacteriophages LSA2308 and LSA2366 showed lytic activity against drug-resistant S. aureus, and combined with ampicillin achieved better clearance in LB broth. In skim milk, a concentration of 10^7 PFU/mL bacteriophages exhibited the most effective bactericidal efficacy against S. aureus strains.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Immunology
Elliot Whittard, James Redfern, Guoqing Xia, Andrew Millard, Roobinidevi Ragupathy, Sladjana Malic, Mark C. Enright
Summary: This study validates the potential of phage therapy for treating Staphylococcus aureus infections by examining the characteristics and host range of lytic S. aureus phages. Phages with the widest host range and effective killing abilities against various isolates were identified, showing promise as therapeutic candidates for future studies.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Alexandra E. Chittams-Miles, Areej Malik, Erin B. Purcell, Claudia Muratori, Brian Conlon
Summary: Treatment with short electric pulses can enhance the effects of multiple antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). This combination therapy reduces the necessary antibiotic dosage and improves the efficacy of treating S. aureus infections such as skin and soft-tissue infections (SSTIs). SSTIs are accessible to physical intervention, making electric pulse co-treatment a potential option for wound and abscess debridement.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Hyerim Kwak, Jinshil Kim, Sangryeol Ryu, Jaewoo Bai
Summary: Phage KMSP1, isolated from raw milk, was characterized as a lytic phage belonging to the Myoviridae family. It showed efficient antimicrobial activity against a broad spectrum of S. aureus strains, including MRSA. The presence of putative tail lysin with antimicrobial activity and the stability of KMSP1 under various conditions suggested its potential as a biocontrol agent.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Microbiology
Lucie Kuntova, Ivana Maslanova, Radka Oborilova, Hana Simeckova, Adela Finstrlova, Pavol Bardy, Marta Siborova, Liudmyla Troianovska, Tibor Botka, Petr Gintar, Ondrej Sedo, Zdenek Farka, Jiri Doskar, Roman Pantucek
Summary: This study describes a resistance mechanism targeting kayviruses that is encoded by a prophage. The mechanism belongs to a broader group of abortive infections, characterized by suicidal behavior of infected cells that are unable to produce phage progeny, thus ensuring the survival of the host population. Since the majority of staphylococcal strains are lysogenic, our findings are relevant for the advancement of phage therapy.
Article
Biochemistry & Molecular Biology
Maria Kornienko, Dmitry Bespiatykh, Maja Malakhova, Roman Gorodnichev, Nikita Kuptsov, Egor Shitikov
Summary: Phage therapy is a hopeful approach to combat multidrug-resistant bacteria. However, it requires the isolation of new phages or the search for suitable phages in existing libraries. This study proposes a simple PCR method to differentiate between two families of Staphylococcus phages and eleven genera of Klebsiella phages, allowing for rapid screening and identification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Eric Brouillette, Guillaume Millette, Suzanne Chamberland, Jean-Pierre Roy, Celine Ster, Tadele Kiros, Stephanie Hickey, Lauren Hittle, Joelle Woolston, Francois Malouin
Summary: Staphylococcus aureus causes chronic mastitis that is difficult to treat with antibiotics. Phage therapy, as an alternative to antibiotics, shows potential in managing mastitis in cows and reducing the spread of antibiotic resistance.
Article
Microbiology
Jun Song, Hongri Ruan, Li Chen, Yuqi Jin, Jiasan Zheng, Rui Wu, Dongbo Sun
Summary: SDQ, a specific lytic S. aureus bacteriophage, can effectively remove S. aureus biofilms. SDQ maintains its full activity under various environmental conditions and can be used in combination with detergents.
Article
Immunology
Manjunath Nandihalli Shetru, Maribasappa Karched, Dayanand Agsar
Summary: The study demonstrated that species-specific phage treatment of Staphylococcus aureus-infected wounds resulted in faster wound healing and 100% survival of the animals, highlighting the potential utility of locally isolated phages against multidrug-resistant MRSA infections.
MICROBIAL PATHOGENESIS
(2021)
Article
Engineering, Biomedical
Ming-Chen Sun, Ying-Fang Chen, Di Liu, Xiao-Ling Xu, Yu-Chan You, Wei Lu, Yun-Juan Shi, Ming-Yang Ren, Yi-Bin Fan, Yong-Zhong Du, Xiao-Hua Tao
Summary: The widespread use of mupirocin for treating MRSA infections has led to the emergence of mupirocin-resistant Staphylococcus aureus (MuRSA), posing a serious medical threat. In order to combat MuRSA, researchers have developed a modified mupirocin and silver complex (TPGS/Mup-Ag) to enhance its antibacterial activity. The results showed that TPGS/Mup-Ag can effectively eliminate bacterial infection and accelerate wound healing in both in vitro and in vivo experiments.
MATERIALS TODAY BIO
(2023)
Article
Biochemistry & Molecular Biology
Nilakshi Barua, Ying Yang, Lin Huang, Margaret Ip
Summary: The inactivation of the VraSR system in Staphylococcus aureus reduces virulence and stress response. In a 3D-skin model, the inactivated VraSR strains showed decreased adhesion, internalization, and apoptosis. In a humanized mouse infection model, the inactivation of VraSR resulted in smaller skin lesions and reduced bacterial burden.
Article
Infectious Diseases
Kevin Simon, Wolfgang Pier, Alex Kruettgen, Hans-Peter Horz
Summary: The combination of Sb-1 phage and oxacillin showed enhanced antibacterial effects against most S. aureus isolates, with rare antagonism observed. This heterologous antimicrobial pair provides a robust approach for controlling MRSA.
Article
Immunology
Miranda J. Ridder, Aubrey K. G. McReynolds, Hongyan Dai, Michele T. Pritchard, Mary A. Markiewicz, Jeffrey L. Bose
Summary: This study examined the host-pathogen interface in a subcutaneous skin infection caused by Staphylococcus aureus. The results showed a dynamic immune response, with high bacterial titers and abundant neutrophils in the early infection stage. Various cytokines were produced, and histopathological analysis revealed extensive cell death and persistent neutrophil infiltration. Detection of IL-17A/F starting at 5 days postinfection coincided with a decrease in bacterial numbers. After 9 days, neutrophils were no longer the most abundant immune cell type, and surface wounds resolved with declining bacterial titers.
INFECTION AND IMMUNITY
(2022)
Article
Biotechnology & Applied Microbiology
Tinghua Li, Lin Lyu, Yan Zhang, Ke Dong, Qingtian Li, Xiaokui Guo, Yongzhang Zhu
Summary: The novel E. thailandicus strain d5B shows effective antimicrobial activity against C. difficile and exhibits probiotic properties, making it a promising candidate for controlling CDI.
Article
Immunology
Jinhong Qin, Nannan Wu, Juan Bao, Xin Shi, Hongyu Ou, Shanke Ye, Wei Zhao, Zhenquan Wei, Jinfeng Cai, Lisha Li, Mingquan Guo, Jingyan Weng, Hongzhou Lu, Demeng Tan, Jianzhong Zhang, Qin Huang, Zhaoqin Zhu, Yejing Shi, Chunlan Hu, Xiaokui Guo, Tongyu Zhu
Summary: This case study illustrates the successful treatment of a patient with multidrug-resistant Klebsiella pneumoniae infections using a cocktail of bacteriophages and antibiotics. The strategy of combining phage therapy with antibiotic treatment proved effective in eradicating the infection. The presence of polyclonal co-infectious cells may pose a challenge in phage therapy, requiring a tailored approach based on individual patient characteristics.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Nannan Wu, Jia Dai, Mingquan Guo, Jianhui Li, Xin Zhou, Feng Li, Yuan Gao, Hongping Qu, Hongzhou Lu, Jing Jin, Tao Li, Lei Shi, Qingguo Wu, Ruoming Tan, Mingli Zhu, Lan Yang, Yun Ling, Shunpeng Xing, Jianzhong Zhang, Bangxin Yao, Shuai Le, Jingmin Gu, Jinhong Qin, Jie Li, Mengjun Cheng, Demeng Tan, Linlin Li, Yiyuan Zhang, Zhaoqin Zhu, Jinfeng Cai, Zhigang Song, Xiaokui Guo, Li-Kuang Chen, Tongyu Zhu
Summary: This study investigated the efficacy of phage therapy in treating secondary bacterial infections in COVID-19 patients. The results showed that phages were able to reduce the burden of CRAB infections, indicating their potential in responding to secondary outbreaks in COVID-19 patients.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Microbiology
Tingting Feng, Sebastian Leptihn, Ke Dong, Belinda Loh, Yan Zhang, Melanie I. Stefan, Mingyue Li, Xiaokui Guo, Zelin Cui
Summary: Phage therapy is a potential treatment option for infections caused by multidrug-resistant bacteria, and the phage JD419 has a broad host range and can be modified through gene engineering to prevent potential lysogeny.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Aman Chandra Kaushik, Qiqi Wu, Li Lin, Haibo Li, Longqi Zhao, Zilu Wen, Yanzheng Song, Qihang Wu, Jin Wang, Xiaokui Guo, Hualin Wang, Xiaoli Yu, Dongqing Wei, Shulin Zhang
Summary: The study indicates that exosomal ncRNAs may serve as useful functional biomarkers in tuberculosis, with miR-185-5p identified as a potential biomarker for tuberculosis.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Immunology
Xiaomei Fu, Lin Lyu, Yue Wang, Yan Zhang, Xiaokui Guo, Qian Chen, Chang Liu
Summary: The study assessed the safety and probiotic characteristics of the newly isolated Enterococcus lactis strain JDM1. JDM1 demonstrated good gastrointestinal tolerance and inhibitory effects on the growth of certain pathogenic bacteria. Its genome contained both resistance-related and virulence-related genes, with resistance genes being mostly conserved and virulence factors not contributing to functional pathogenicity.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Mufeng Cai, Bingchun Pu, Yue Wang, Lin Lv, Chunyu Jiang, Xiaomei Fu, Yan Zhang, Wei Zhao, Ke Dong, Yi Yang, Yangming Liu, Yalu Wei, Zhengyue Zhang, Jianhui Li, Xiaokui Guo, Chang Liu, Jinhong Qin
Summary: Klebsiella pneumoniae exhibits extensive phenotypic and genetic diversity, with plasmids playing a key role in its genome diversity. The plasmid p2 was found to be packaged into membrane vesicles instead of phage particles, and its absence led to increased plasmid uptake, phage burst, and virulence. Further study on this mechanism will help us understand how it provides adaptive phenotypes to host evolution.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Microbiology
Cheng Chang, Xinbo Yu, Wennan Guo, Chaoyi Guo, Xiaokui Guo, Qingtian Li, Yongzhang Zhu
Summary: Biofilms are complex microbial microcolonies that are resistant to antibiotics and can adhere to various surfaces. Using bacteriophages and their components for biofilm removal shows promise, but there are still challenges to overcome.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Xiao-Xi Zhang, Jing-Shu Liu, Le-Fei Han, Shang Xia, Shi-Zhu Li, Odel Y. Li, Kokouvi Kassegne, Min Li, Kun Yin, Qin-Qin Hu, Le-Shan Xiu, Yong-Zhang Zhu, Liang-Yu Huang, Xiang-Cheng Wang, Yi Zhang, Han-Qing Zhao, Jing-Xian Yin, Tian-Ge Jiang, Qin Li, Si-Wei Fei, Si-Yu Gu, Fu-Min Chen, Nan Zhou, Zi-Le Cheng, Yi Xie, Hui-Min Li, Jin Chen, Zhao-Yu Guo, Jia-Xin Feng, Lin Ai, Jing-Bo Xue, Qian Ye, Liz Grant, Jun-Xia Song, Geoff Simm, Jurg Utzinger, Xiao-Kui Guo, Xiao-Nong Zhou
Summary: This study proposes the development of a global One Health index (GOHI) as a potential evaluation tool for measuring and assessing One Health performance. The GOHI framework includes external drivers, intrinsic drivers, and core drivers, and is constructed based on multiple rounds of expert discussions. The pilot analysis using data from over 200 countries/territories shows that the overall GOHI scores are far from ideal, indicating the urgent need for capacity building in One Health. The study suggests that GOHI could be a feasible tool for assessing and promoting One Health approach.
INFECTIOUS DISEASES OF POVERTY
(2022)
Article
Biology
Lin Lyu, Ru Feng, Mingnan Zhang, Xiaoqing Xie, Yinjing Liao, Yanjiao Zhou, Xiaokui Guo, Bing Su, Yair Dorsett, Lei Chen
Summary: This article describes the identification of a core sgRNA repertoire for SARS-CoV-2 and the discovery of novel sgRNAs. The study also found that the ratio of Spike sgRNA to Nucleocapsid sgRNA is highest in SARS-CoV-2, among the examined beta-coronaviruses. It was further observed that this ratio can be adjusted by modifying the viral RNA replication machinery.
LIFE SCIENCE ALLIANCE
(2022)
Article
Infectious Diseases
Yongzhang Zhu, Li Shi, Yige Zeng, Dongri Piao, Yingbo Xie, Juan Du, Meng Gao, Wei Gao, Junli Tian, Jun Yue, Min Li, XiaoKui Guo, Yufeng Yao, YaoXia Kang
Summary: This study investigates the immune characteristics of different stages of brucellosis and finds that CD8(+)T cells and TLR4 may be closely associated with the progression of the disease.
INFECTIOUS DISEASES OF POVERTY
(2022)
Article
Infectious Diseases
Nan Zhou, Zile Cheng, Xiaoxi Zhang, Chao Lv, Chaoyi Guo, Haodong Liu, Ke Dong, Yan Zhang, Chang Liu, Yunfu Chang, Sheng Chen, Xiaokui Guo, Xiao-Nong Zhou, Min Li, Yongzhang Zhu
Summary: This study developed a comprehensive tool for assessing global antimicrobial resistance (AMR) status and evaluated AMR in 146 countries. The study found that high-income countries performed better in terms of AMR, while low-income countries unexpectedly performed well in certain indicators. The study also identified correlations between AMR and socioeconomic factors such as GDP and life expectancy.
INFECTIOUS DISEASES OF POVERTY
(2022)
Review
Chemistry, Multidisciplinary
Huimin Li, Yi Xie, Fumin Chen, Huiwen Bai, Leshan Xiu, Xiaonong Zhou, Xiaokui Guo, Qinqin Hu, Kun Yin
Summary: Rapid and accurate molecular diagnosis is crucial for precision medicine, food safety, and environmental monitoring. CRISPR/Cas-based detection has become an effective tool for molecular diagnosis due to its outstanding advantages. However, existing methods typically require pre-amplification, and the development of target amplification-free CRISPR/Cas-based detection still faces challenges.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Green & Sustainable Science & Technology
Yi Xie, Huimin Li, Jingshu Liu, Lefei Han, Xiaoxi Zhang, Xiaonong Zhou, Xiaokui Guo, Leshan Xiu, Hao Yin, Kun Yin
Summary: This study developed the CCIR index, which is the first index to explore variations in climate risks, impacts, and responses across countries to identify vulnerabilities and find more targeted solutions. The study found a positive correlation between the CCIR index and national GDP per capita, indicating wealthier countries allocate more resources toward mitigating climate impacts. Additionally, countries with better climate education tend to have a lower carbon footprint, and countries with lower risks of emerging infectious diseases consume more renewable energy.
INTERNATIONAL JOURNAL OF SUSTAINABLE DEVELOPMENT AND WORLD ECOLOGY
(2023)
Article
Immunology
Yanchun Wang, Xia Fan, Lin Du, Boyu Liu, Haihan Xiao, Yan Zhang, Yunqiang Wu, Fuli Liu, Yung-Fu Chang, Xiaokui Guo, Ping He
Summary: While SR-A1 aids in the uptake of Leptospira by macrophages, it may be utilized by the pathogen to modulate inflammatory responses and increase susceptibility to infection in the host.
EMERGING MICROBES & INFECTIONS
(2021)