期刊
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES
卷 84, 期 8, 页码 331-344出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15287394.2020.1867275
关键词
(-)-epigallocatechin-3-gallate; superoxide dismutase; total antioxidant capacity; glutathione; hexavalent chromium; apoptosis; 8-hydroxydeoxyguanosine; genotoxic damage
资金
- DGAPA-UNAM, Support Program for Research and Technological Innovation Projects (PAPIIT) [IN224719]
This study revealed that EGCG can reduce oxidative stress induced by chromium(VI) by increasing 8-OHdG levels and SOD activity, thereby reducing DNA damage and cell apoptosis. Pre-administration of EGCG before CrO3 treatment effectively reduced micronucleus formation, decreased apoptotic and necrotic cell numbers, and restored intracellular antioxidant capacity and SOD activity.
This study aimed to investigate the relationship between endogenous antioxidant system, 8-hydroxydeoxyguanosine adduct (8-OHdG) repair, and apoptosis in mice treated with chromium(VI) alone and in the presence of the antigenotoxic compound (-)-epigallocatechin-3-gallate (EGCG). Groups of 5 Hsd:ICR male mice were divided and treated as follows: (1) control, vehicle only; (2) EGCG, 8.5 mg/kg by gavage alone; (3) CrO3, 20 mg/kg intraperitoneally alone; and (4) EGCG combined with CrO3, EGCG was administered 4 hr prior to CrO3. Peripheral blood parameters were analyzed before treatment administration (time 0), and 48 hr after exposure. The administration of EGCG increased 8-OHdG levels and superoxide dismutase (SOD) activity. Treatment with CrO3 increased number of micronucleus (MN) presence, elevated apoptotic/necrotic cells frequencies, decreased 8-OHdG levels, diminished total antioxidant capacity (TAC), increased glutathione (GSH) total levels, and lowered SOD activity. Administration of EGCG prior to treatment with CrO3 resulted in lower concentrations of MN, reduced apoptotic and necrotic cell number, and restored TAC and SOD activity to control levels. It is conceivable that the dose of EGCG plays an important role in the genotoxic damage protection pathways. Thus, this study confirms the action of EGCG as an antigenotoxic agent against chromium(VI)-induced oxidative insults and demonstrates potential protective pathways for EGCG actions to counteract genotoxic damage induced by this metal.
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