期刊
DIGESTIVE DISEASES AND SCIENCES
卷 61, 期 8, 页码 2252-2261出版社
SPRINGER
DOI: 10.1007/s10620-016-4145-y
关键词
Claudin-3; Interleukin-1 beta; beta Catenin; Non-muscle myosin light chain kinase; Epithelial barrier; Permeability
资金
- US Department of Veterans' Affairs [BX000799]
- National Institutes of Health [R01 HL096640, HL120954]
IL-1 beta is a cytokine involved in mediating epithelial barrier dysfunction in the gut. It is known that IL-1 beta mediates activation of non-muscle myosin light chain kinase in epithelial cells, but the precise mechanism by which epithelial barrier dysfunction is induced by IL-1 beta is not understood. Using a Caco2 cell model, we show that the expression of the tight junction protein, claudin-3, is transcriptionally downregulated by IL-1 beta treatment. In addition, after assessing protein and mRNA expression, and protein localization, we show that inhibition of nmMLCK rescues IL-1 beta-mediated decrease in claudin-3 expression as well as junction protein redistribution. Using chromatin immunoprecipitation assays, we also show that beta-catenin targeting of the claudin-3 promoter occurs as a consequence of IL-1 beta-mediated epithelial barrier dysfunction, and inhibition of nmMLCK interferes with this interaction. Taken together, these data represent the first line of evidence demonstrating nmMLCK regulation of claudin-3 expression in response to IL-1 beta-treated epithelial cells.
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