期刊
JOURNAL OF HEMATOLOGY & ONCOLOGY
卷 13, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13045-020-01009-7
关键词
Osteopontin; Integrin; EGFR-TKI; Resistance; NSCLC
资金
- National Natural Science Foundation of China [81702870, 81802885, 81972174, 82073213]
- Jiangsu Provincial Medical Youth Talent [QNRC2016746]
- Gusu Youth Health Talent of Suzhou [2020-082]
- Suzhou Key Laboratory for Respiratory Medicine [SZS201617]
- Clinical Medical Center of Suzhou [Szzx201502]
- Jiangsu Provincial Key Medical Discipline [ZDXKB2016007]
- Clinical Key Specialty Project of China
Background Acquired epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) resistance limits the long-term clinical efficacy of tyrosine kinase-targeting drugs. Although most of the mechanisms of acquired EGFR-TKI resistance have been revealed, the mechanism of similar to 15% of cases has not yet been elucidated. Methods Cell viability was analysed using the Cell Counting Kit-8 (CCK-8) assay. Proteome profiler array analysis was performed to find proteins contributing to acquired EGFR-TKI resistance. Secreted OPN was detected by ELISA. Immunohistochemical analysis was conducted to detect expression of integrin alpha V in NSCLC tissue. The effect of VS-6063 on apoptosis and proliferation of PC9 gefitinib-resistant cells was detected by fluorescence-activated cell sorting (FACS) and clonogenic assays. A mouse xenograft model was used to assess the effect of VS-6063 on the sensitivity of PC9 gefitinib-resistant cells to gefitinib. Results OPN was overexpressed in acquired EGFR-TKI-resistant NSCLCs. Secreted OPN contributed to acquired EGFR-TKI resistance by activating the integrin alpha V beta 3/FAK pathway. Inhibition of FAK signalling increased sensitivity to EGFR-TKIs in PC9 gefitinib-resistant cells both in vitro and in vivo. Conclusions OPN contributes to acquired EGFR-TKI resistance by up-regulating expression of integrin alpha V beta 3, which activates the downstream FAK/AKT and ERK signalling pathways to promote cell proliferation in NSCLC.
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