Article
Microbiology
Maria D. Flores-Chavez, Alba Abras, Cristina Ballart, Ismael Ibanez Perez, Pilar Perez-Gordillo, Montserrat Gallego, Carmen Munoz, Zaira Moure, Elena Sulleiro Igual, Javier Nieto, Emilia Garcia Diez, Israel Cruz, Albert Picado
Summary: The study evaluated the performance of the Loopamp Trypanosoma cruzi detection kit for the diagnosis of congenital and chronic Chagas disease. The results showed high sensitivity and accuracy for congenital CD, but lower accuracy for chronic CD. Tcruzi-LAMP can be useful for screening and early diagnosis of congenital infection.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Chemistry, Applied
Yanbo Yu, Rui Li, Zonghua Ma, Meihong Han, Sen Zhang, Minghui Zhang, Youwen Qiu
Summary: In this study, a novel LAMP-TaqMan detection method was developed for quick qualitative detection of genetically modified organisms (GMOs). This method showed high specificity and was able to detect target sequences with as few as five copies within approximately 20 minutes. It was successfully applied to amplify and detect DNA from food samples of major crops, offering a rapid approach for GMO detection in foods.
Article
Plant Sciences
Junye Jiang, Will Feindel, Michael Harding, David Feindel, Stacey Bajema, Jie Feng
Summary: In this study, a loop-mediated isothermal amplification (LAMP) method was developed and evaluated for the detection of S. subterranea, the causal agent of powdery scab of potato. Tests showed that the LAMP method has high specificity and sensitivity, making it a useful tool for rapid and accurate detection of S. subterranea and confirmation of powdery scab disease.
Review
Medicine, General & Internal
Xiaoling Zhang, Yongjuan Zhao, Yi Zeng, Chiyu Zhang
Summary: This article reviews the applications of probe-based LAMP assays in pathogen detection, discusses their features and advantages over traditional LAMP methods.
Article
Plant Sciences
Lina Yang, Liang Zhang, Jun Cao, Lingyun Wang, Hengsong Shi, Feng Zhu, Zhaolin Ji
Summary: In this study, a rapid and sensitive detection method for the peach shoot blight (PSB) pathogen Phomopsis amygdali using loop-mediated isothermal amplification (LAMP) technology was developed. The LAMP assay showed higher detection rates and sensitivity compared to conventional PCR. The results provide important experimental evidence for the identification and prevention of PSB, and can aid in disease control and management.
Article
Microbiology
Maurice A. Matthew, Jevan Christie, Nawu Yang, Chaoqun Yao
Summary: Trichomonas tenax, a protozoan found in the oral cavity, can cause poor oral hygiene and periodontal disease. A newly developed LAMP assay was 1000 times more sensitive than conventional PCR, with high specificity and the ability to provide a fast, cheap, and reliable diagnostic tool for T. tenax. This assay has potential applications in medical and veterinary clinics.
Article
Infectious Diseases
Jessica Vanhomwegen, Aurelia Kwasiborski, Abou Diop, Laure Boizeau, Damien Hoinard, Muriel Vray, Raymond Bercion, Babacar Ndiaye, Amelie Dublineau, Satoru Michiyuki, Jean-Claude Manuguerra, Virginie Sauvage, Daniel Candotti, Abdoulaye Seck, Syria Laperche, Yusuke Shimakawa
Summary: A simple, rapid, and inexpensive alternative to PCR, the HBV-LAMP assay, has been developed to diagnose high viraemia >= 200,000 IU/mL, which may help in identifying high-risk pregnant women in resource-limited countries to eliminate HBV mother-to-child transmission.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Infectious Diseases
Masato Inaba, Yuki Higashimoto, Yoko Toyama, Tomoya Horiguchi, Masaya Hibino, Mitsunaga Iwata, Kazuyoshi Imaizumi, Yohei Doi
Summary: The study demonstrates that RT-LAMP has a diagnostic accuracy comparable to RT-qPCR in the early phase of COVID-19, until the 9th day after symptom onset. However, after the 10th day, the positivity rate of RT-LAMP significantly decreases, suggesting its limitations in diagnosing COVID-19 in later stages of the disease.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Veterinary Sciences
Muhammad Arfan Zaman, Azhar Rafique, Uzma Mehreen, Saba Mehnaz, Farhan Ahmad Atif, Asghar Abbas, Kashif Hussain, Muhammad Asif Raza, Sidra Altaf, Faisal Siddique, Rahman Md. Masudur, Mosaab Omar
Summary: A LAMP assay was developed for the diagnosis of T. lestoquardi, a major cause of ovine theileriosis. The assay showed high specificity and sensitivity and can be used as a diagnostic tool for the disease.
PAKISTAN VETERINARY JOURNAL
(2022)
Article
Plant Sciences
Junye Jiang, Will Feindel, Kylie Swisher Grimm, Michael Harding, David Feindel, Stacey Bajema, Jie Feng
Summary: In this study, a loop-mediated isothermal amplification (LAMP) method was developed and evaluated for the detection of 'Candidatus Liberibacter solanacearum' (Lso) haplotypes A and B. The LAMP-A and LAMP-B primers showed high specificity to Lso and generated positive signals only from Lso-infected tomato DNA mixtures. The researchers highly recommend the LAMP-A and LAMP-B primers in combination with the LAMP technique for rapid, accurate, and direct differentiation of Lso haplotypes A and B.
Article
Infectious Diseases
Thanyarat Promlek, Maytawan Thanunchai, Uraporn Phumisantiphong, Tonsan Hansirisathit, Chayanit Phuttanu, Sunisa Dongphooyao, Wipawee Thongsopa, Pornlada Nuchnoi
Summary: The clinical performance of the FastProof 30 min-TTR SARS-CoV-2 RT-LAMP method was validated in this study. The results showed that RT-LAMP had high sensitivity and specificity in detecting COVID-19 viral RNA and has the potential for mass screening of patients with high viral load.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Fisheries
Zhongjie Zan, Zhuoran Han, Yuting Hou, Yanyu Zhang, Jingfeng Sun
Summary: In this study, a rapid, specific and sensitive loop mediated isothermal amplification (LAMP) method was successfully established for the detection of Shewanella algae in aquatic animals. The method showed high specificity, and through adjustment of primer concentration and optimization of reaction conditions, rapid diagnosis can be achieved.
AQUACULTURE REPORTS
(2022)
Review
Psychiatry
Yanmei Xu, Xinyu Pan, Huan Li, Qiongfang Cao, Fan Xu, Jianshu Zhang
Summary: This study aimed to evaluate the accuracy of Raman spectroscopy in the diagnosis of Alzheimer's disease. Eight studies were included in the meta-analysis, and the results showed that Raman spectroscopy had a sensitivity of 0.86, specificity of 0.87, positive likelihood ratio of 5.50, negative likelihood ratio of 0.17, diagnosis odds ratio of 42.44, and area under the curve of 0.931. Sensitivity analysis indicated the stability of the results. In conclusion, Raman spectroscopy has high accuracy in the diagnosis of AD, but the possibility of misdiagnosis and missed diagnosis still exists. More high-quality studies are needed to verify the conclusions.
FRONTIERS IN PSYCHIATRY
(2023)
Article
Infectious Diseases
Shan Gunasegar, Vasantha Kumari Neela
Summary: LAMP is more sensitive and accurate in diagnosing leptospirosis compared to PCR. However, significant heterogeneity was observed, indicating the need for further work to reduce heterogeneity and improve effective intervention strategies.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2021)
Review
Oncology
Tuan Trong Luu
Summary: Pancreatic cancer is a deadly cancer type with poor prognosis and high mortality rate, making early detection a focus of academic research. Immunohistochemistry biomarkers are believed to play a crucial role in improving diagnostic efficacy for pancreatic cancer.
FRONTIERS IN ONCOLOGY
(2021)
Article
Virology
Alix Armero, Nicolas Berthet, Jean-Christophe Avarre
Summary: Following the identification of SARS-CoV-2 as the cause of COVID-19, efforts have been made to study the genetic diversity within hosts through quasispecies analysis. Research in Victoria, Australia found quasispecies diversity in 68% of samples, with a majority being nonsynonymous variants, particularly in the spike glycoprotein and ORF1a genes. Shared variants among samples suggested host-to-host transmission, with implications for the virus's epidemiology.
Review
Fisheries
Andrew C. Barnes, Oleksandra Silayeva, Matt Landos, Ha Thanh Dong, Angela Lusiastuti, Le Hong Phuoc, Jerome Delamare-Deboutteville
Summary: Antimicrobial resistance is a global crisis, especially in aquaculture where antibiotics are often used without proper information and record-keeping. Vaccination with autogenous vaccines may be a cost-effective solution to reduce antibiotic use, particularly in low- and middle-income countries with limited veterinary support.
REVIEWS IN AQUACULTURE
(2022)
Article
Fisheries
Uni Purwaningsih, Sukenda Sukenda, Angela M. Lusiastuti, Alimuddin Alimuddin, Widanarni Widanarni, Sri Nuryati
Summary: This study compared the phenotypic, genotypic characteristics, and pathogenicity of Edwardsiella ictaluri from different catfish farms in Indonesia. The results showed variations in growth patterns, enzyme activity, antibiotic resistance, and genetic profiles among the isolates. The protein components of E. ictaluri cells were found to be virulent and played a role in the pathogenesis of E. ictaluri infection in catfish.
AQUACULTURE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Ming Wen, Qiaowei Pan, Elodie Jouanno, Jerome Montfort, Margot Zahm, Cedric Cabau, Christophe Klopp, Carole Iampietro, Celine Roques, Olivier Bouchez, Adrien Castinel, Cecile Donnadieu, Hugues Parrinello, Charles Poncet, Elodie Belmonte, Veronique Gautier, Jean-Christophe Avarre, Remi Dugue, Rudhy Gustiano, Tran Thi Thuy Ha, Marc Campet, Kednapat Sriphairoj, Josiane Ribolli, Fernanda L. de Almeida, Thomas Desvignes, John H. Postlethwait, Christabel Floi Bucao, Marc Robinson-Rechavi, Julien Bobe, Amaury Herpin, Yann Guiguen
Summary: This study generated novel genomic resources for 12 Pangasiids, characterized their sex determination system, and identified a conserved master sex-determining gene. The findings contribute to a better understanding of the dynamics of sex determination systems.
MOLECULAR ECOLOGY RESOURCES
(2022)
Article
Immunology
Sandro Klafack, Lars Schroeder, Yeonhwa Jin, Matthias Lenk, Pei-Yu Lee, Walter Fuchs, Jean-Christophe Avarre, Sven M. Bergmann
Summary: In this study, a live attenuated vaccine virus was generated and shown to protect carp or koi against wild type challenge. Vaccination induced mild clinical signs and produced protecting antibodies.
Article
Ecology
Juliet Lamb, Jeremy Tornos, Romain Dedet, Hubert Gantelet, Nicolas Keck, Juliette Baron, Marine Bely, Augustin Clessin, Aline Flechet, Amandine Gamble, Thierry Boulinier
Summary: Wildlife movement ecology studies often focus on breeders, neglecting the movements of nonbreeding individuals. Loafing areas provide an alternative to breeding sites for capturing and studying nonbreeding individuals. Our study reveals that nonbreeders have wider ranges, are more active, and interact more with other species compared to breeders. Nonbreeders also have higher exposure to pathogens and forage in breeding areas, facilitating interspecific pathogen transmission.
FUNCTIONAL ECOLOGY
(2023)
Article
Environmental Sciences
Elodie Pepey, Genevieve Conejero, Elia Njara Ndrianaivo, Jean-Luc Verdeil, Jean-Michel Mortillaro, David Boggio, Modestine Raliniaina, Simon Pouil
Summary: This study evaluated the cytogenotoxic potential of water from urban and rural Malagasy marshes using fish and plants as bioindicators. The results showed DNA strand breaks in fish erythrocytes in both marshes, while potential cytotoxicity was mainly observed in the roots of plants in the urban marsh. These findings demonstrate the advantages of coupling in vivo biological test systems to assess cytogenotoxicity in surface water in low-income countries.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2023)
Article
Microbiology
Marine Combe, Miriam Reverter, Domenico Caruso, Elodie Pepey, Rodolphe Elie Gozlan
Summary: With increasing population, food security is a significant concern for the future. The impact of food production on the environment has prompted the evaluation of shifting diets from meat to fish and seafood for environmental and health benefits. The spread of infectious animal diseases in a warming climate poses a risk to sustainable aquaculture development. A meta-analysis reveals a positive correlation between global warming and viral virulence, indicating a potential threat to aquaculture and global food security.
Article
Agriculture, Dairy & Animal Science
Mira Mawardi, Agustin Indrawati, I. Wayan Teguh Wibawan, Angela Mariana Lusiastuti
Summary: This study aimed to isolate bacteria as potential producers of extracellular enzymes, phenotypic characterization, and antibiotic-resistant gene patterns. The study found 137 bacterial isolates that can produce extracellular enzymes and are sensitive to antibiotics, serving as potential probiotics. Fifteen isolates were resistant to oxytetracycline, seven isolates were resistant to erythromycin, three isolates were resistant to tetracycline, and one isolate was resistant to enrofloxacin. Four isolates exhibited multidrug resistance.
Article
Virology
Jean Delmotte, Camille Pelletier, Benjamin Morga, Richard Galinier, Bruno Petton, Jean-Baptiste Lamy, Oliver Kaltz, Jean-Christophe Avarre, Maude Jacquot, Caroline Montagnani, Jean-Michel Escoubas
Summary: This study investigated the genetic diversity of the causative agent of Pacific oyster mortality syndrome, OsHV-1, using deep sequencing and bioinformatics tools. The results revealed connectivity between OsHV-1 viral populations in different oyster-farming areas, with oyster transfers in France playing a major role in its dispersal.
Article
Fisheries
Yani Aryati, Widanarni Widanarni, Dinamella Wahjuningrum, Iman Rusmana, Angela Mariana Lusiastuti
Summary: The study revealed that honey, used as a prebiotic, significantly improved the growth performance, intestinal health, and microbiota diversity in Nile tilapia.
AQUACULTURE RESEARCH
(2021)