期刊
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
卷 86, 期 1, 页码 30-34出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2016.06.002
关键词
Carbapenemases; RT-PCR; CPE
Carbapenemase-producing Enterobacteriaceae (CPE) represent an increasing public health issue and the early detection of colonization by CPE can help the implementation of infection control measures among inpatients. In this study, BD MAX Check-Direct CPE screen, with two different Master Mixes (BDMix and CPMix), using the automatic BD MAX (TM) instrument, was evaluated for the detection of bla(KPC), bla(OXA-48), bla(VIM) and bla(NDM) genes, in comparison to selective broth enrichment and direct culture from rectal swabs. Among a total of 557 rectal swabs samples, 29 (5.2%) tested positive for CPE (23 for bla(KPC) 5 for bla(VIM) and one for bla(OXC-48)). The sensitivity, specificity, positive and negative likelihood ratios values were 93.1%, 973%, 34.5 and 0.07, for BMix, and 100%, 97.1 %, 34.5 and 0 for CPMix, respectively. Five samples were positive with molecular methods only. The turn-around time was reduced from 18-24 hours (direct culture), or 48 h (broth enrichment) to only 3 h. (C) 2016 Elsevier Inc. All rights reserved.
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