Article
Pharmacology & Pharmacy
Venkatesh Mayandi, Wen-Tyng Kang, Darren Shu Jeng Ting, Eunice Tze Leng Goh, Myoe Naing Lynn, Thet Tun Aung, Jamuna Vadivelu, Veluchamy Amutha Barathi, Anita Sook Yee Chan, Rajamani Lakshminarayanan
Summary: This study demonstrates that the combination of propranolol and antifungal drugs can enhance antifungal activity, particularly in combination with itraconazole. In vivo studies using a murine systemic candidemia model showed that the combination treatment resulted in decreased weight loss, lower kidney fungal burden, and reduced renal inflammation compared to monotherapy or no treatment. These findings suggest that propranolol can increase the efficacy of azoles against C. albicans, providing a new therapeutic strategy for invasive fungal infections.
Article
Biochemistry & Molecular Biology
Kang Ding, Panpan Shen, Zhongwen Xie, Lifang Wang, Xiangli Dang
Summary: Candida albicans infections often require novel antifungal drugs. The potential antimicrobial peptide P256 demonstrated antifungal activity against C. albicans, showing synergism when combined with amphotericin B, and exerting multiple effects on the fungi.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2022)
Article
Microbiology
Bin Zhu, Zhen Li, Hongmei Yin, Jun Hu, Yingjun Xue, Guanyi Zhang, Xin Zheng, Weiqin Chen, Xiaobo Hu
Summary: This study demonstrates, for the first time, the inhibitory effect of pseudolaric acid A (PAA) alone and in combination with fluconazole (FLC) on Candida albicans biofilms. The combination of PAA and FLC shows an improved antibiofilm effect by inhibiting adhesion and yeast-to-hypha transition. These findings provide new insights into the mechanisms of antifungal drug combinations and offer potential strategies for addressing C. albicans drug resistance.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Sarah R. Beattie, Taiwo Esan, Robert Zarnowski, Emily Eix, Jeniel E. Nett, David R. Andes, Timothy Hagen, Damian J. Krysan
Summary: New antifungal therapies are needed for various fungal infections, including biofilms and drug-resistant strains. A novel keto-alkyl-pyridinium scaffold has been identified with broad spectrum activity against medically important fungi. This class of molecules shows potential for non-systemic applications.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Medicine, Research & Experimental
Yuting Wang, Chunyan Lu, Xia Zhao, Decai Wang, Yaxin Liu, Shujuan Sun
Summary: This study found that Asiatic acid in combination with fluconazole had a synergistic effect on fluconazole-resistant Candida albicans both in vitro and in vivo. The combination reduced the minimum inhibitory concentration of fluconazole and inhibited hyphal growth, possibly through mechanisms such as inhibition of drug efflux pump and accumulation of reactive oxygen species. These findings provide new insights for overcoming drug resistance and offer potential for future development and application of Asiatic acid.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Microbiology
Rabina Kumpakha, Donna M. Gordon
Summary: In this study, the researchers demonstrated the efficacy of occidiofungin against biofilm development of Candida albicans and Candida tropicalis, including inhibiting cell dispersal. The results suggest that occidiofungin has strong antibiofilm activity and potential as a therapeutic for Candida infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Public, Environmental & Occupational Health
S. Umamaheshwari, M. N. Sumana
Summary: This study retrospectively analyzed the distribution of Candida in various clinical samples in a tertiary care hospital in India. It found that urine samples were the major source of Candida infection, and non-albicans Candida (NAC) was the predominant species. The susceptibility test revealed that about 75.44% of isolates were susceptible to the tested drugs, while about 24.56% were resistant to one or more drugs.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Biochemistry & Molecular Biology
Lady Daiane Pereira Leite, Maria Alcioneia Carvalho de Oliveira, Mariana Raquel da Cruz Vegian, Aline da Graca Sampaio, Thalita Mayumi Castaldelli Nishime, Konstantin Georgiev Kostov, Cristiane Yumi Koga-Ito
Summary: The study showed that cold atmospheric plasma (CAP) had a more effective antifungal effect on Candida albicans biofilms compared to nystatin and amphotericin B, and also exhibited some effects when used in combination with conventional polyene antifungal agents.
Article
Immunology
Jie Tu, Tianbao Zhu, Qingwen Wang, Wanzhen Yang, Yahui Huang, Defeng Xu, Na Liu, Chunquan Sheng
Summary: Candida auris is a serious public health threat that has caused a global epidemic due to its multi-drug resistance, remarkable transmissibility, and high mortality. A novel benzoanilide antifungal agent, compound A1, was discovered through a comprehensive strategy involving phenotypic screening, hit optimization, antifungal assays, and mechanism exploration. Compound A1 showed potent efficacy against Candida auris infection both in vitro and in vivo. Mechanistic investigation revealed that compound A1 inhibited the biosynthesis of virulence factors and fungal cell walls by blocking glycosylphosphatidylinositol (GPI) and GPI-anchored proteins. Therefore, compound A1 represents a promising lead compound for combating drug-resistant candidiasis.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Microbiology
Katura Metzner, Matthew J. O'Meara, Benjamin Halligan, Jesse W. Wotring, Jonathan Z. Sexton, Teresa R. O'Meara
Summary: Fungal pathogens like Candida albicans can cause devastating human disease, but there is high resistance to antifungal therapies. Targeting virulence factors is a new approach to antimicrobial development, and we identified NSC 697923 as a compound that blocks the hyphal transition in C. albicans by targeting eIF3.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Jia Zhou, Jinyang Li, Iohong Cheong, Ning-Ning Liu, Hui Wang
Summary: This study identified artemether and its derivatives as having antifungal activity, with artemether showing the strongest effect in combination with fluconazole against resistant strains. The combination of artemether and fluconazole also demonstrated broad-spectrum antifungal activity against various fungal species. Mechanistic investigations suggest that artemether may enhance fluconazole efficacy by disrupting specific cellular functions.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Georgiana Negru, Laure Kamus, Elena Bicu, Sergiu Shova, Boualem Sendid, Faustine Dubar, Alina Ghinet
Summary: The reaction between benzylidenemalononitriles and phenylhydrazines in refluxing ethanol did not lead to pyrazole derivatives, but instead produced hydrazones, whose structure was confirmed by X-ray analysis. The newly synthesized hydrazones showed promising antifungal activity against Candida spp., with five compounds demonstrating selective antifungal activity with MIC99 values ranging from 16 to 32 μg/mL.
Review
Biochemistry & Molecular Biology
Gabriela Vieira Silva Zolin, Fauller Henrique da Fonseca, Carolina Reis Zambom, Saulo Santesso Garrido
Summary: Buccal candidiasis, caused by Candida albicans, can lead to systemic infection with resistance to common antifungal drugs. Histatin 5, a natural antimicrobial peptide found in human saliva, shows high antifungal activity against C. albicans. Research aims to explore the promising use of Hst5 metallopeptides in developing effective drugs to overcome antifungal resistance.
Article
Chemistry, Medicinal
Martina Raschig, Bernardo Ramirez-Zavala, Johannes Wiest, Marco Saedtler, Marcus Gutmann, Ulrike Holzgrabe, Joachim Morschhauser, Lorenz Meinel
Summary: Increasing resistance to antifungal drugs poses a challenge to anti-infective therapies and contributes to mortality in infections caused by drug-resistant Candida species and strains. This study explores the use of naturally occurring molecules as lead structures for the development of new antifungal agents. The synthesized azobenzene derivatives showed inhibitory effects on both fluconazole-susceptible and fluconazole-resistant Candida species, indicating their potential therapeutic value.
ARCHIV DER PHARMAZIE
(2023)
Article
Medicine, General & Internal
Thomas Voit, Fabian Cieplik, Johannes Regensburger, Karl-Anton Hiller, Anita Gollmer, Wolfgang Buchalla, Tim Maisch
Summary: The study investigated the localization of TMPyP in Candida albicans before and after irradiation with visible light to understand the antifungal mechanism of aPDT. Results showed that the photosensitizer accumulated in the cell envelope before light irradiation, and entered the cytosol after treatment, suggesting damage to the cell wall. This finding may contribute to the development of more effective PS for fungal infections.
FRONTIERS IN MEDICINE
(2021)
Article
Engineering, Environmental
Betina Sayeg Burd, Cassamo Ussemane Mussagy, Junya de Lacorte Singulani, Jean Lucas Tanaka, Mateus Scontri, Giovana Sant'Ana Pegorin Brasil, Nayrim Brizuela Guerra, Patricia Akemi Assato, Ana Paula De Sousa Abreu, Camila Calderan Bebber, Maira Terra-Garcia, Juliana Campos Junqueira, Neda Farhadi, Ana Marisa Fusco Almeida, Maria Jose Soares Mendes-Giannini, Giannini, Bingbing Li, Rondinelli Donizetti Herculano
Summary: Excessive plastic production and improper disposal have led to unprecedented pollution. This study found that Galleria mellonella larvae have high biodegradation ability for polystyrene (PS) and low-density polyethylene (LDPE), making them a promising and cost-effective alternative.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2023)
Article
Engineering, Biomedical
Mayara Zaghi Dal Picolo, Matheus Kury, Diego Romario-Silva, Pedro Luiz Rosalen, Vanessa Gallego Arias Pecorari, Marcelo Gianinni, Vanessa Cavalli
Summary: The objective of this study was to evaluate the effects of simulated gastric acid erosion combined with mechanical toothbrushing abrasion on the mechanical properties, surface topography, and biofilm adhesion of different CAD/CAM materials. The results showed that the combination of erosive challenges and abrasion negatively influenced the mechanical properties and surface topography of most CAD/CAM materials and increased the biofilm adhesion on ZLS.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Review
Pharmacology & Pharmacy
Lais de Almeida Campos, Margani Taise Fin, Kelvin Sousa Santos, Marcos William de Lima Gualque, Ana Karla Lima Freire Cabral, Najeh Maissar Khalil, Ana Marisa Fusco-Almeida, Rubiana Mara Mainardes, Maria Jose Soares Mendes-Giannini
Summary: Invasive fungal infections have become a significant cause of mortality and morbidity worldwide. The current treatment options are limited due to low bioavailability and toxicity. Voriconazole is widely used to treat various fungal infections, but its non-linear pharmacokinetics and individual variations in metabolism can lead to supratherapeutic doses and increased toxicity. Recent research has explored the use of nanotechnology-based drug delivery systems to improve the delivery of voriconazole. These systems have shown promising results in enhancing drug permeation and deposition in target tissues, allowing for controlled and sustained release in different administration routes. Overall, nanotechnology-based drug delivery systems offer a more effective and safer alternative for treating fungal infections.
Article
Pharmacology & Pharmacy
Caroline B. Costa-Orlandi, Niura M. Bila, Jean Lucas C. Bonatti, Carolina O. Vaso, Mariana B. Santos, Carlos R. Polaquini, Mariana M. Santoni M. Biasioli, Rondinelli D. Herculano, Luis O. Regasini, Ana Marisa Fusco-Almeida, Maria Jose S. Mendes-Giannini
Summary: The ability of dermatophytes to form biofilms and resist antifungal drugs has led to treatment recurrence. In this study, the susceptibility and mechanism of action of nonyl 3,4-dihydroxybenzoate (nonyl) on planktonic cells and biofilms of T. rubrum and T. mentagrophytes were evaluated. The results showed that nonyl effectively damaged the biofilms, while synthetic drugs had little effect and even promoted the development of resistant structures. Confocal and transmission electron microscopy revealed a reduction in biofilm thickness and disruption of the plasma membrane. These findings support the potential of nonyl as a promising antifungal compound.
Article
Microbiology
Kelvin Sousa dos Santos, Lariane Teodoro Oliveira, Marina de Lima Fontes, Ketylin Fernanda Migliato, Ana Marisa Fusco-Almeida, Maria Jose Soares Mendes Giannini, Andrei Moroz
Summary: A 3D lung aggregate model using sodium alginate scaffolds was developed for studying interactions between Paracoccidioides brasiliensis and lung epithelial cells. The model showed low cytotoxicity, increased cell density, and maintained cell viability for seven days. Adding ECM proteins to the scaffolds resulted in a significantly higher number of retrieved fungi.
Article
Infectious Diseases
Dalviany Taques Ferreira, Priscila Vieira da Silva, Helio Chagas Chaves de Oliveira Junior, Kellyth Andrade Pereira Rocha, Diego Romario da Silva, Nayla de Souza Pitangui, Janaina de Cassia Orlandi Sardi
Summary: This article highlights the potential relationship between oral candidiasis and candidemia through a literature review, emphasizing the importance of prevention to reduce hospitalization time and improve patients' quality of life.
CURRENT FUNGAL INFECTION REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Isabela Jacob Moro, Flavio Alexandre Carvalho, Juhan Augusto Scardelato Pereira, Gabriela Brasil Romao, Andre Faibicher, Winner Duque Rodrigues, Renata Pires Assis, Iguatemy Lourenco Brunetti, Junya de Lacorte Singulani, Ana Marisa Fusco-Almeida, Andre Gonzaga dos Santos
Summary: This study used UHPLC-UV green chromatography to analyze the antioxidant and anti-inflammatory activities of Varronia curassavica, as well as its embryotoxicity in Zebrafish. The results showed that certain components in the leaves of V. curassavica exhibited good antioxidant and anti-inflammatory activities, but also had some toxic effects on Zebrafish embryos.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Microbiology
Mariana C. Galeane, Paulo C. Gomes, Junya L. Singulani, Maria J. S. Mendes-Giannini, Ana M. Fusco-Almeida
Summary: In this study, an IsCT analogue peptide (PepM3) was designed based on structural studies of wasp mastoparans and its antifungal activity and toxicity were evaluated. The results showed that PepM3 exhibited activity against Candida albicans and synergistic effects with amphotericin B. The peptide had fungicidal action, low toxicity in human skin keratinocytes, and was nonteratogenic in zebrafish embryos.
FUTURE MICROBIOLOGY
(2023)
Article
Food Science & Technology
Gisele Goulart da Silva, Lucia Elaine de Oliveira Braga, Ellen Cristina Souza de Oliveira, Joao Ernesto de Carvalho, Josy Goldoni Lazarini, Pedro Luiz Rosalen, Ana Paula Dionisio, Ana Lucia Tasca Gois Ruiz
Summary: The standardized extract (CAE) obtained from cashew apple bagasse shows potential beneficial effects on ulcerative colitis. CAE is more effective in delaying disease progression but cannot prevent or inhibit the disease. The concentration of carotenoids in CAE may not be sufficient, and there may be an antagonistic relationship between carotenoids and anacardic acids.
Article
Microbiology
Evelyn Pulido-Camarillo, Jorge H. Sahaza, Nayla de Souza Pitangui, Maria Jose S. Mendes-Giannini, Ana M. Fusco-Almeida, Armando Perez-Torres, Maria Lucia Taylor
Summary: This study revealed differential host pulmonary inflammatory responses associated with distinct genotypes of H. capsulatum strains during infection. Mice infected with the LAm A2 strain showed progressive inflammation in the lung parenchyma during the early stages of infection, and granulomas with macrophages containing yeast cells at 14 and 21 days post-infection. In contrast, infection with the NAm 2 strain primarily induced bronchiolar-associated lymphoid tissue formation.
Review
Microbiology
Ana Marisa Fusco-Almeida, Samanta de Matos Silva, Kelvin Sousa dos Santos, Marcos William de Lima Gualque, Carolina Orlando Vaso, Angelica Romao Carvalho, Kaila Petrolina Medina-Alarcon, Ana Carolina Moreira da Silva Pires, Jenyffie Araujo Belizario, Ligia de Souza Fernandes, Andrei Moroz, Luis R. Martinez, Orville Hernandez Ruiz, Angel Gonzalez, Maria Jose Soares Mendes-Giannini
Summary: Due to global legislation restricting the use of mammalian animals in research, alternative methods have gained importance in the study of fungal pathogenesis. This review discusses the use of three-dimensional cultures and alternative animal models to study host-fungus interactions.
Article
Microbiology
Caroline Maria Marcos, Haroldo Cesar de Oliveira, Patricia Akemi Assato, Lariane Teodoro de Oliveira, Nathalia Fregonezi, Kelvin Sousa dos Santos, Caroline Barcelos Costa-Orlandi, Ana Marisa Fusco-Almeida, Maria Jose Soares Mendes-Giannini
Summary: This study explores signaling pathways implicated in fungal pathobiology and identifies the crucial role of the Drk1 protein in the morphological shift and virulence of Paracoccidioides brasiliensis. Peptides with affinity for PbDrk1 were found to inhibit the phase transition and adhesion of P. brasiliensis, and augment the effects of antifungal agents. The peptides also showed enhanced larval survival rates in a Galleria mellonella model. Further investigation of PbDrk1 may improve our understanding of P. brasiliensis and lead to the development of novel therapeutic strategies.
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
